Abstract: It provides general information about the Chatkal nature reserve (Uzbekistan), its physico-geographical features, and description of flora and fauna. Fauna of vertebrate animals includes four fishes, two amphibians, 11 reptiles, 176 birds, and 33 mammals. The typical mammals of the nature reserve are fox and badger. Sometimes, steppe cat can be met too. Snow leopard and marbled polecat are seen rarely. Brown bear is quite widely distributed. Ungulates such as wild boar and ibex are widely distributed in the nature reserve.