Abstract: A syndrome of multiple congenital ocular anomalies in a litter of domestic kittens is described which appears identical to the multiple colobomatous syndrome described in captive Snow Leopards. The lesions varied between kittens in the litter, but ranged from microphthalmos with blindness to mild alterations in the lateral lid margins that resulted in trichiasis. The syndrome of eyelid agenesis in the domestic cat may encompass a broad range of congenital ocular lesions and multiple siblings, but the cause and mechanism of lesion formation is unknown.