Abstract: Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species.

Abstract: Liver tissues from 126 captive cheetah were evaluated by light microscopy and histochemistry; eight animals were evaluated by electron microscopy. The main hepatic lesion, a vascular lesion resembling veno- occlusive disease (VOD) of the liver and characterized by subendothelial fibrosis and proliferation of smooth muscle-like cells in the central veins, was seen in 60% of the sexually mature cheetah. Although this hepatic vascular lesion was seen in cheetah as young as 1 year of age, the most severe lesions, usually associated with liver failure, were found in cheetah between the ages of 6 and 11. There was no sex predisposition, and in approximately 40% of the VOD cases, liver disease was not suspected clinically or at necropsy. VOD was found in other felidae, especially in the snow leopard. High levels of vitamin A in livers, as well as in diets of the cheetah, could be a contributing factor in the development of VOD in some groups of cheetah.

Abstract: Generally speaking, men and women are the two wheels of the same vehicle. Women's
participation and involvement in the conservation of snow leopards is as indispensable as it is to
any other discipline. Unfortunately, their dual role was not exploited and so the involvement of
women in nature conservation is considered almost inappropriate and not up to the mark.
Mountainous people still live with their centuries old traditions and culture. Although with the
passage of time they are leaning towards modernization, still most of them are associated with
the centuries-old tradition of keeping livestock. These people take their livestock to the pastures
and thus share in the snow leopard's habitat. A large number of livestock grazing in the snow
leopard's habitat attracts this opportunistic animal to attack domestic animals. This is the point
where the conflict between snow leopards and livestock owners starts, with the snow leopard
killed in retaliation. Snow leopard depredation causes economic losses to the herders and
women, being the managers of the house, take the maximum impact. Keeping this fact in view,
a study was undertaken in the two demonstration sites of the snow leopard project in Chitral and
Gilgit. A specific questionnaire was used and information was collected from all the women
herders in the two demo sites. The study revealed that women have positive attitudes toward and
feel love for this precious animal.

Abstract: The potential for rescuing immature oocytes from the ovaries of females of rare felid species which die or undergo medical ovariohysterectomy was evaluated. Ovaries were recovered from 13 species representing 35 individuals in good-to-poor health. Although the majority of females were 10 yr of age or older and in fair-to-poor health, a total of 846 oocytes were recovered of which 608 (71.9%) were classified as fair-to- excellent quality. One hundred of these oocytes were used for initial maturation classification and as parthogenetic controls. Overall, of the 508 fair-to-excellent quality oocytes placed in culture, 164 (32.3%) matured to metaphase II in vitro. For species in which 3 or more individuals yielded oocytes, mean oocyte maturation rates were as follows: 36.2%, tiger; 27.9% leopard; and 8.3%, cheetah. In vitro insemination of oocytes resulted in fertilization (2 polar bodies, 2 pronuclei, or cleavage) rates of 9.1% to 28.6% (leopard) using homologous fresh spermatozoa and 4.0% (lion) to 40.0% (puma) using homologous frozen-thawed spermatozoa. Inseminations using heterologous (domestic cat) spermatozoa also resulted in fertilized oocytes in the tiger, leopard, snow leopard, puma, serval, and Geoffroy's cat (range in fertilization rate, 5.0% for leopard to 46.2% for puma). Cleaved embryos resulted from the insemination of leopard oocytes with homologous sperm (n = 1 embryo) and puma oocytes with domestic cat sperm (n = 3 embryos). These results demonstrate that immature ovarian oocytes from rare felid species can be stimulated to mature in vitro despite an excision-to-culture interval as long as 36 h.(ABSTRACT TRUNCATED AT 250 WORDS)

Abstract: This paper deals with the geographical distribution of ounces (Panthera uncia) in Qinghai Province. Ounces are distributed in 20 counties- Guide, Huzhu, Menyuan, Qilian, Tianjun, Dulan, Golmud, Guinan, Xinghai, Zhidoi, Zadoi, Nangqen, Yushu, Chindu, Qumarleb, Madio, Maqen, Jigzhi, Baima, Darlag. Among them, there fore 4 counties- Qilian, Tianjun, Dulan, Zadoi, in which the number of ounces are bigger. The number of ounces are shown in table 2. There are altogether 73 ounces (40 male, 33 female) which is supported to every park of China for ornamental, they were captured by fellow-villagers, and 44 ounces (23 male, 21 female) of them are below 6 months old, 9 ounces (6 male, 3 female) of them are 1 year old, 2 ounces (male) are 2 years old, and 18 ounces (9 male, 9 female) are adults.
Ounces live at an altitude of 3000-4100 metres above the sea, and prefer to eat Bharal (Pseudois noyour). Its breeding period goes from April to June, the number of embryos being 2-3.
A female ounce was successfully reproduced for the first time at Xining People's Park of China, in Spetember, 1984, and she gave birth to 3 young ounces.