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| Author | Janecka; J.E.; Jackson, R.; Yuquang, Z.; Diqiang, L.; Munkhtsog, B.; Buckley-Beason, V.; Murphy, W.J. | ||||
| Title | Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study | Type | Miscellaneous | ||
| Year | 2008 | Publication | Animal Conservation | Abbreviated Journal | |
| Volume | 11 | Issue | Pages | 401-411 | |
| Keywords | snow leopard; genetics; scat; noninvasive; survey. | ||||
| Abstract | The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards. | ||||
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| Notes | Supplemental information may be found at http:snowleopardnetwork.org/bibliography/JaneckaSupplemental2008.pdf | Approved | no | ||
| Call Number | SLN @ rana @ 883 | Serial | 488 | ||
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| Author | Janovsky, M.; Grone, A.; Ciardo, D.; Vollm, J.; Burnens, A.; Fatzer, R.; Bacciarini, L.N. | ||||
| Title | Phaeohyphomycosis in a Snow Leopard (Uncia uncia) due to Cladophialophora bantiana | Type | Miscellaneous | ||
| Year | 2006 | Publication | Journal of Comparative Pathology | Abbreviated Journal | |
| Volume | 134 | Issue | Pages | 245-248 | |
| Keywords | Cladophialophora bantiana; fungal infection; phaeohyphomycosis; snow leopard; spinal meningitis; Uncia uncia | ||||
| Abstract | Phaeohyphomycosis caused by Cladophialophora bantiana was diagnosed in a 5-month-old snow leopard with spastic paralysis of the hind legs and inability to defaecate or urinate. At post-mortem examination, a greenish soft mass resembling an abscess was found on one side of the epidural space at the fourth lumbar vertebral body. Histological examination revealed a purulent meningitis with myelomalacia. Dematiaceous fungal hyphae, present within the inflammatory infiltrate, were identified as C. bantiana by culture and sequence analysis of the 18S ribosomal RNA gene. This neurotropic fungus rarely affects organs other than the brain in human beings and cats, and has been reported only occasionally in Europe. The case described suggests that phaeohyphomycosis due to C. bantiana infection may be recognized more frequently in the future and the possible involvement of organs other than the brain should be borne in mind. | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ 891 | Serial | 489 | ||
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| Author | Jegal, A.; Kashkarov, E.; Matyushkin E.N. | ||||
| Title | Simple method to distinguish tracks of snow leopard and lynx | Type | Manuscript | ||
| Year | 2010 | Publication | Abbreviated Journal | ||
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| Keywords | tracks; snow; snow leopard; snow-leopard; leopard; Lynx; gobi; Altai; mountain; range; mountains; region; distribution; Case; local; hunters; hunter; Animals; Animal | ||||
| Abstract | In the Mongolian and Gobi Altai mountain ranges and also in some other mountains in this region, the distribution of the snow leopard and Eurasian lynx overlaps. In some cases, local hunters cannot distinguish the tracks of both these animals. Therefore we outline a simple method to distinguish tracks of the snow leopard and lynx. |
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| Notes | Two English translations of article are provided. | Approved | no | ||
| Call Number | SLN @ rana @ 1101 | Serial | 491 | ||
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| Author | Jiang, Z. | ||||
| Title | Snow leopards in the Dulan International Hunting Ground, Qinghai, China | Type | Report | ||
| Year | 2005 | Publication | Abbreviated Journal | ||
| Volume | Issue | Pages | 1-8 | ||
| Keywords | snow; snow leopards; snow leopard; snow-leopards; snow-leopard; leopards; leopard; International; hunting; Qinghai; China; project; international snow leopard trust; International-Snow-Leopard-Trust; trust; program; surveys; survey; mountains; mountain; province; transect; study; area; transects; pug; pug marks; pug-marks; marks; scrapes; scrape; density; densities; wild; ungulates; ungulate; region; camera; environment; photo; capture; population; population size; population-size; Animals; Animal; 20; livestock; Human; attitudes; attitude; tibetan; 30; nature; reserve; uncia; Uncia uncia; Uncia-uncia; species; snow line; snow-line; endemic; alpine; central; Central Asia; asia; countries; country; fox; range; areas; Xinjiang; inner; Inner-Mongolia; Mongolia; Tibet; gansu; Sichuan; habitat; protection; nature reserves; reserves; cat; populations; domestic; laws; law; field; field surveys; field survey; field-surveys; field-survey; Kunlun; distribution; survival; status; Data; conservation | ||||
| Abstract | From March to May, 2006œªwe conducted extensive snow leopard surveys in the Burhanbuda Mountain Kunlun Mountains, Qinghai Province, China. 32 linear transect of 5~15 km each, which running through each vegetation type, were surveyed within the study area. A total of 72 traces of snow leopard were found along 4 transects (12.5% of total transects). The traces included pug marks or footprints, scrapes and urine marks. We estimated the average density of wild ungulates in the region was 2.88ñ0.35 individuals km-2(n=29). We emplaced 16 auto2 trigger cameras in different environments and eight photos of snow leopard were shot by four cameras and the capture rate of snow leopard was 71.4%. The minimum snow leopard population size in the Burhanbuda Mountain was two, because two snow leopards were phototrapped by different cameras at almost same time. Simultaneously, the cameras also shot 63 photos of other wild animals, including five photos are unidentified wild animals, and 20 photos of livestock. We evaluated the human attitudes towards snow leopard by interviewing with 27 Tibetan householders of 30 householders live in the study area. We propose to establish a nature reserve for protecting and managing snow leopards in the region. Snow leopard (Uncia uncia) is considered as a unique species because it lives above the snow line, it is endemic to alpines in Central Asia, inhabiting in 12 countries across Central Asia (Fox, 1992). Snow leopard ranges in alpine areas in Qinghai, Xinjiang, Inner Mongolia, Tibet, Gansu and Sichuan in western China (Liao, 1985, 1986; Zhou, 1987; Ma et al., 2002; Jiang & Xu, 2006). The total population and habitat of snow leopards in China are estimated to be 2,000~2,500 individuals and 1,824,316 km2, only 5% of which is under the protection of nature reserves. The cat's current range is fragmented (Zou & Zheng, 2003). Due to strong human persecutions, populations of snow leopards decreased significantly since the end of the 20th century. Thus, the snow leopards are under the protection of international and domestic laws. From March to May, 2006, we conducted two field surveys in Zhiyu Village, Dulan County in Burhanbuda Mountain, Kunlun Mountains, China to determine the population, distribution and survival status of snow leopards in the area. The aim of the study was to provide ecologic data for snow leopard conservation. |
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| Notes | Project funded by International Snow Leopard Trust Small Grants Program. | Approved | no | ||
| Call Number | SLN @ rana @ 1068 | Serial | 493 | ||
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| Author | Jie, Z.; Zongwei, W. | ||||
| Title | Qinghai Fauna | Type | Journal Article | ||
| Year | 1963 | Publication | Journal of Animal | Abbreviated Journal | |
| Volume | 15 | Issue | 1 | Pages | 125-137 |
| Keywords | China; Qinghai; status; distribution; browse; 3420 | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ 20 | Serial | 495 | ||
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| Author | Johnson, D. | ||||
| Title | The National Fish and Wildlife Foundation goes international | Type | Journal Article | ||
| Year | 1994 | Publication | Endangered Species Update, A | Abbreviated Journal | |
| Volume | 11 | Issue | 10 | Pages | A10 |
| Keywords | Nfwf; India; asia; siberia; endangered-species; snow-leopard; managemnet; conservation; snow leopard; browse; endangered; species; 1150 | ||||
| Abstract | Abstract: The National Fish and Wildlife Foundation (NFWF) which is a conservation organization created in 1984 aims to conserve the species on an international context before they are endangered which will enable a more effective conservation procedure. The NFWF has addressed the causes of endangered species in India and South Asia such as the tiger, Indian wolf and the snow leopard and has supported the conservation efforts of the Siberian tiger. It has cooperated with multi-national organizations to evaluate the best strategy that could be adopted to prevent a future extinction of several species and has supported CITES programs | ||||
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| Call Number | SLN @ rana @ 254 | Serial | 500 | ||
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| Author | Johnson, W.E.; Dratch, P.A.; Martenson, J.S.; O'Brien, S.J. | ||||
| Title | Resolution of recent radiations within three evolutionary lineages of Felidae using mitochondrial restriction fragment length polymorphism variation | Type | Journal Article | ||
| Year | 1996 | Publication | Journal of Mammalian Evolution | Abbreviated Journal | |
| Volume | 3 | Issue | 2 | Pages | 97-120 |
| Keywords | Evolution; Evolutionary-Lineages; Mitochondrial-Dna; Mitochondrial-Restriction-Fragment-Length-Polymorphism-Variation; phylogeny; Recent-Radiations; Restriction-Enzymes; Tigrina; snow-leopard; browse; evolutionary; lineages; mitochondrial; Dna; restriction; fragment; length; polymorphism; variation; radiation; enzymes; recent; recent radiation; 1340 | ||||
| Abstract | Patterns of mitochondrial restriction fragment length polymorphism (RFLP) variation were used to resolve more recent relationships among the species of the Felidae ocelot lineage, domestic cat lineage, and pantherine lineage. Twenty-five of 28 restriction enzymes revealed site variation in at least 1 of 21 cat species. The ocelot lineage was resolved into three separate sister taxa groups: Geoffroy's cat (Oncifelis geoffroyi) and kodkod (O. guigna), ocelot (Leopardus pardalis) and margay (L. wiedii), and pampas cat (Lynchailurus colocolo) and most of the tigrina samples (Leopardus tigrina). Within the domestic cat lineage, domestic cat (Felis catus), European wild cat (F. silvestris), and African wild cat (F. libyca) formed a monophyletic trichotomy, which was joined with sand cat (F. margarita) to a common ancestor. Jungle cat (F. chaus) and black-footed cat (F. nigripes) mtDNAs diverged earlier than those of the other domestic cat lineage species and are less closely related. Within the pantherine lineage, phylogenetic analysis identified two distinct groups, uniting lion (P. leo) with leopard (P. pardus) and tiger (P. tigris) with snow leopard (P. uncia). | ||||
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| Notes | Document Type: English Call Number: QL708.5 J68 | Approved | no | ||
| Call Number | SLN @ rana @ 276 | Serial | 501 | ||
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| Author | Johnson, W.E.; Eizirik, E.; Pecon-Slattery, J.; Murphy, W.J.; Antunes, A.; Teeling, E.; O'Brien, S.J. | ||||
| Title | The Late Miocene Radiation of Modern Felidae: A Genetic Assessment | Type | Miscellaneous | ||
| Year | 2006 | Publication | Science | Abbreviated Journal | |
| Volume | 311 | Issue | Pages | 74-77 | |
| Keywords | carnivore; classification; divergence; Dna; Felidae; fossil; lineages; Miocene; mitochondrial; Molecular; phylogeny; radiation; taxonomic | ||||
| Abstract | Modern felid species descend from relatively recent (G11 million years ago) divergence and speciation events that produced successful predatory carnivores worldwide but that have confounded taxonomic classifications. A highly resolved molecular phylogeny with divergence dates for all living cat species, derived from autosomal, X-linked, Y-linked, and mitochondrial gene segments (22,789 base pairs) and 16 fossil calibrations define eight principal lineages produced through at least 10 intercontinental migrations facilitated by sea-level fluctuations. A ghost lineage analysis indicates that available felid fossils underestimate (i.e., unrepresented basal branch length) first occurrence by an average of 76%, revealing a low representation of felid lineages in paleontological remains. The phylogenetic performance of distinct gene classes showed that Y-chromosome segments are appreciably more informative than mitochondrial DNA, X-linked, or autosomal genes in resolving the rapid Felidae species radiation. | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ 908 | Serial | 502 | ||
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| Author | Johnston, L.A.; Donoghue, A.M.; O'Brien, S.J.; Wildt, D.E. | ||||
| Title | Rescue and maturation in vitro of follicular oocytes collected from nondomestic felid species | Type | Journal Article | ||
| Year | 1991 | Publication | Biol Reprod | Abbreviated Journal | |
| Volume | 45 | Issue | 6 | Pages | 898-906 |
| Keywords | Animal; Carnivora; anatomy; histology; physiology; Cells; Cultured; Female; Fertilization; Vitro; inVitro; Male; Oocytes; cytology; growth; development; ovarian; Follicle; Spermatozoa; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 230 | ||||
| Abstract | The potential for rescuing immature oocytes from the ovaries of females of rare felid species which die or undergo medical ovariohysterectomy was evaluated. Ovaries were recovered from 13 species representing 35 individuals in good-to-poor health. Although the majority of females were 10 yr of age or older and in fair-to-poor health, a total of 846 oocytes were recovered of which 608 (71.9%) were classified as fair-to- excellent quality. One hundred of these oocytes were used for initial maturation classification and as parthogenetic controls. Overall, of the 508 fair-to-excellent quality oocytes placed in culture, 164 (32.3%) matured to metaphase II in vitro. For species in which 3 or more individuals yielded oocytes, mean oocyte maturation rates were as follows: 36.2%, tiger; 27.9% leopard; and 8.3%, cheetah. In vitro insemination of oocytes resulted in fertilization (2 polar bodies, 2 pronuclei, or cleavage) rates of 9.1% to 28.6% (leopard) using homologous fresh spermatozoa and 4.0% (lion) to 40.0% (puma) using homologous frozen-thawed spermatozoa. Inseminations using heterologous (domestic cat) spermatozoa also resulted in fertilized oocytes in the tiger, leopard, snow leopard, puma, serval, and Geoffroy's cat (range in fertilization rate, 5.0% for leopard to 46.2% for puma). Cleaved embryos resulted from the insemination of leopard oocytes with homologous sperm (n = 1 embryo) and puma oocytes with domestic cat sperm (n = 3 embryos). These results demonstrate that immature ovarian oocytes from rare felid species can be stimulated to mature in vitro despite an excision-to-culture interval as long as 36 h.(ABSTRACT TRUNCATED AT 250 WORDS) | ||||
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| ISSN | 0006-3363 | ISBN | Medium | ||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 172 | Serial | 503 | ||
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| Author | Johnston, L.A.; Armstrong, D.L.; Brown, J.L. | ||||
| Title | Seasonal effects on seminal and endocrine traits in the captive snow leopard (Panthera uncia) | Type | Journal Article | ||
| Year | 1994 | Publication | J Reprod Fertil | Abbreviated Journal | |
| Volume | 102 | Issue | 1 | Pages | 229-236 |
| Keywords | Animal; Carnivora; physiology; Comparative; study; Fsh; Blood; Gonadotropins; Pituitary; Lh; Male; Seasons; Semen; Sperm; Motility; Spermatozoa; cytology; Testosterone; browse; 180 | ||||
| Abstract | The annual reproductive cycle of the male snow leopard (Panthera uncia) was characterized by evaluating seminal and endocrine traits monthly. Testicular volume was greatest (P < 0.05) during the winter months when the quality of ejaculate was optimal. Ejaculate volume, total sperm concentration ml-1, motile sperm concentration per ejaculate, sperm morphology and sperm motility index were lowest during the summer and autumn months compared with the winter and spring. Peripheral LH, FSH and testosterone concentrations were also lowest during the summer months, increasing during the autumn just before the increase in semen quality, and were maximal during the winter months. There was a direct relationship (P < 0.01) between: (1) testosterone and testicular volume, total sperm concentration ml-1, motile sperm concentration per ejaculate and ejaculate volume, and (2) LH and testicular volume and motile sperm concentration per ejaculate. In summary, although spermatozoa were recovered throughout the year, optimal gamete quality was observed during the winter and spring. Although previous studies in felids have demonstrated seasonal effects on either seminal or endocrine traits, this is the first study to demonstrate a distinct effect of season on both pituitary and testicular function. | ||||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 252 | Serial | 504 | ||
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