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Author (up) Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. url  openurl
  Title Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples Type Journal Article
  Year 2014 Publication Conservation Genetic Resource Abbreviated Journal  
  Volume 6 Issue 2 Pages 369:373  
  Keywords Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification  
  Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic

information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many

noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the

domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard

populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and

population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers

facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.

endangered species
 
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number SLN @ rakhee @ Serial 1427  
Permanent link to this record
 

 
Author (up) Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. url  openurl
  Title Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples Type Journal Article
  Year 2014 Publication Conservation Genetic Resource Abbreviated Journal  
  Volume 6 Issue 2 Pages 369:373  
  Keywords Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification  
  Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic

information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many

noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the

domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard

populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and

population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers

facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.
 
  Address  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number SLN @ rakhee @ Serial 1428  
Permanent link to this record
 

 
Author (up) Janjua,S., Peters, J. L., Weckworth, B., Abbas, F. I., Bahn, Volker, Johansson, O., Rooney, T.P. url  openurl
  Title Improving our conservation genetic toolkit: ddRAD-seq for SNPs in snow leopards Type Journal Article
  Year 2019 Publication Conservation Genetic Resource Abbreviated Journal  
  Volume Issue Pages  
  Keywords  
  Abstract Snow leopards (Panthera uncia) are an enigmatic, high-altitude species whose challenging habitat, low population densities

and patchy distribution have presented challenges for scientists studying its biology, population structure, and genetics.

Molecular scatology brings a new hope for conservation efforts by providing valuable insights about snow leopards, including

their distribution, population densities, connectivity, habitat use, and population structure for assigning conservation units.

However, traditional amplification of microsatellites from non-invasive sources of DNA are accompanied by significant

genotyping errors due to low DNA yield and poor quality. These errors can lead to incorrect inferences in the number of

individuals and estimates of genetic diversity. Next generation technologies have revolutionized the depth of information

we can get from a species' genome. Here we used double digest restriction-site associated DNA sequencing (ddRAD-seq),

a well-established technique for studying non-model organisms, to develop a reference sequence library for snow leopards

using blood samples from five Mongolian individuals. Our final data set reveals 4504 loci with a median size range of 221 bp.

We identified 697 SNPs and low nucleotide diversity (0.00032) within these loci. However, the probability that two random

individuals will share identical genotypes is about 10-168. We developed probes for DNA capture using this sequence library

which can now be used for genotyping individuals from scat samples. Genetic data from ddRAD-seq will be invaluable for

conducting population and landscape scale studies that can inform snow leopard conservation strategies.
 
  Address Snow leopard · ddRAD-seq · Next generation sequencing · SNP discovery  
  Corporate Author Thesis  
  Publisher Place of Publication Editor  
  Language Summary Language Original Title  
  Series Editor Series Title Abbreviated Series Title  
  Series Volume Series Issue Edition  
  ISSN ISBN Medium  
  Area Expedition Conference  
  Notes Approved no  
  Call Number SLN @ rakhee @ Serial 1483  
Permanent link to this record
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