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Author Janecka; J.E.; Jackson, R.; Yuquang, Z.; Diqiang, L.; Munkhtsog, B.; Buckley-Beason, V.; Murphy, W.J.
Title Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study Type Miscellaneous
Year 2008 Publication Animal Conservation Abbreviated Journal
Volume 11 Issue Pages 401-411
Keywords snow leopard; genetics; scat; noninvasive; survey.
Abstract The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards.
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Notes Supplemental information may be found at http:snowleopardnetwork.org/bibliography/JaneckaSupplemental2008.pdf Approved no
Call Number SLN @ rana @ 883 Serial 488
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Author Janovsky, M.; Grone, A.; Ciardo, D.; Vollm, J.; Burnens, A.; Fatzer, R.; Bacciarini, L.N.
Title Phaeohyphomycosis in a Snow Leopard (Uncia uncia) due to Cladophialophora bantiana Type Miscellaneous
Year 2006 Publication Journal of Comparative Pathology Abbreviated Journal
Volume 134 Issue Pages 245-248
Keywords Cladophialophora bantiana; fungal infection; phaeohyphomycosis; snow leopard; spinal meningitis; Uncia uncia
Abstract Phaeohyphomycosis caused by Cladophialophora bantiana was diagnosed in a 5-month-old snow leopard with spastic paralysis of the hind legs and inability to defaecate or urinate. At post-mortem examination, a greenish soft mass resembling an abscess was found on one side of the epidural space at the fourth lumbar vertebral body. Histological examination revealed a purulent meningitis with myelomalacia. Dematiaceous fungal hyphae, present within the inflammatory infiltrate, were identified as C. bantiana by culture and sequence analysis of the 18S ribosomal RNA gene. This neurotropic fungus rarely affects organs other than the brain in human beings and cats, and has been reported only occasionally in Europe. The case described suggests that phaeohyphomycosis due to C. bantiana infection may be recognized more frequently in the future and the possible involvement of organs other than the brain should be borne in mind.
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Notes Approved no
Call Number SLN @ rana @ 891 Serial 489
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Author Jegal, A.; Kashkarov, E.; Matyushkin E.N.
Title Simple method to distinguish tracks of snow leopard and lynx Type Manuscript
Year 2010 Publication Abbreviated Journal
Volume Issue Pages
Keywords tracks; snow; snow leopard; snow-leopard; leopard; Lynx; gobi; Altai; mountain; range; mountains; region; distribution; Case; local; hunters; hunter; Animals; Animal
Abstract In the Mongolian and Gobi Altai mountain ranges and also in some other mountains in this region, the

distribution of the snow leopard and Eurasian lynx overlaps. In some cases, local hunters cannot

distinguish the tracks of both these animals. Therefore we outline a simple method to distinguish tracks of

the snow leopard and lynx.
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Notes Two English translations of article are provided. Approved no
Call Number SLN @ rana @ 1101 Serial 491
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Author Jiang, Z.
Title Snow leopards in the Dulan International Hunting Ground, Qinghai, China Type Report
Year 2005 Publication Abbreviated Journal
Volume Issue Pages 1-8
Keywords snow; snow leopards; snow leopard; snow-leopards; snow-leopard; leopards; leopard; International; hunting; Qinghai; China; project; international snow leopard trust; International-Snow-Leopard-Trust; trust; program; surveys; survey; mountains; mountain; province; transect; study; area; transects; pug; pug marks; pug-marks; marks; scrapes; scrape; density; densities; wild; ungulates; ungulate; region; camera; environment; photo; capture; population; population size; population-size; Animals; Animal; 20; livestock; Human; attitudes; attitude; tibetan; 30; nature; reserve; uncia; Uncia uncia; Uncia-uncia; species; snow line; snow-line; endemic; alpine; central; Central Asia; asia; countries; country; fox; range; areas; Xinjiang; inner; Inner-Mongolia; Mongolia; Tibet; gansu; Sichuan; habitat; protection; nature reserves; reserves; cat; populations; domestic; laws; law; field; field surveys; field survey; field-surveys; field-survey; Kunlun; distribution; survival; status; Data; conservation
Abstract From March to May, 2006œªwe conducted extensive snow leopard surveys in the Burhanbuda Mountain Kunlun Mountains, Qinghai Province, China. 32 linear transect of 5~15 km each, which running through each vegetation type, were surveyed within the study area. A total of 72 traces of snow leopard were found along 4 transects (12.5% of total transects). The traces included pug marks or footprints, scrapes and urine marks. We estimated the average density of wild ungulates in the region was 2.88ñ0.35 individuals km-2(n=29). We emplaced 16 auto2 trigger cameras in different environments and eight photos of snow leopard were shot by four cameras and the capture rate of snow leopard was 71.4%. The minimum snow leopard population size in the Burhanbuda Mountain was two, because two snow leopards were phototrapped by different cameras at almost same time. Simultaneously, the cameras also shot 63 photos of other wild animals, including five photos are unidentified wild animals, and 20 photos of livestock. We evaluated the human attitudes towards snow leopard by interviewing with 27 Tibetan householders of 30 householders live in the study area. We propose to establish a nature reserve for protecting and managing snow leopards in the region. Snow leopard (Uncia uncia) is considered as a unique species because it lives above the snow line, it is endemic to alpines in Central Asia, inhabiting in 12 countries across Central Asia (Fox, 1992). Snow leopard ranges in alpine areas in Qinghai, Xinjiang, Inner Mongolia, Tibet, Gansu and Sichuan in western China (Liao, 1985, 1986; Zhou, 1987; Ma et al., 2002; Jiang & Xu, 2006). The total population and habitat of snow leopards in China are estimated to be 2,000~2,500 individuals and 1,824,316 km2, only 5% of which is under the protection of nature reserves. The cat's current range is fragmented (Zou & Zheng, 2003). Due to strong human persecutions, populations of snow leopards decreased significantly since the end of the 20th century. Thus, the

snow leopards are under the protection of international and domestic laws. From March to May, 2006, we conducted two field surveys in Zhiyu Village, Dulan County in Burhanbuda Mountain, Kunlun Mountains, China to determine the population, distribution and survival status of snow leopards in the area. The aim of the study was to provide ecologic data for snow leopard conservation.
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Notes Project funded by International Snow Leopard Trust Small Grants Program. Approved no
Call Number SLN @ rana @ 1068 Serial 493
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Author Johnson, D.
Title The National Fish and Wildlife Foundation goes international Type Journal Article
Year 1994 Publication Endangered Species Update, A Abbreviated Journal
Volume 11 Issue 10 Pages A10
Keywords Nfwf; India; asia; siberia; endangered-species; snow-leopard; managemnet; conservation; snow leopard; browse; endangered; species; 1150
Abstract Abstract: The National Fish and Wildlife Foundation (NFWF) which is a conservation organization created in 1984 aims to conserve the species on an international context before they are endangered which will enable a more effective conservation procedure. The NFWF has addressed the causes of endangered species in India and South Asia such as the tiger, Indian wolf and the snow leopard and has supported the conservation efforts of the Siberian tiger. It has cooperated with multi-national organizations to evaluate the best strategy that could be adopted to prevent a future extinction of several species and has supported CITES programs
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Notes Approved no
Call Number SLN @ rana @ 254 Serial 500
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Author Johnson, W.E.; Dratch, P.A.; Martenson, J.S.; O'Brien, S.J.
Title Resolution of recent radiations within three evolutionary lineages of Felidae using mitochondrial restriction fragment length polymorphism variation Type Journal Article
Year 1996 Publication Journal of Mammalian Evolution Abbreviated Journal
Volume 3 Issue 2 Pages 97-120
Keywords Evolution; Evolutionary-Lineages; Mitochondrial-Dna; Mitochondrial-Restriction-Fragment-Length-Polymorphism-Variation; phylogeny; Recent-Radiations; Restriction-Enzymes; Tigrina; snow-leopard; browse; evolutionary; lineages; mitochondrial; Dna; restriction; fragment; length; polymorphism; variation; radiation; enzymes; recent; recent radiation; 1340
Abstract Patterns of mitochondrial restriction fragment length polymorphism (RFLP) variation were used to resolve more recent relationships among the species of the Felidae ocelot lineage, domestic cat lineage, and pantherine lineage. Twenty-five of 28 restriction enzymes revealed site variation in at least 1 of 21 cat species. The ocelot lineage was resolved into three separate sister taxa groups: Geoffroy's cat (Oncifelis geoffroyi) and kodkod (O. guigna), ocelot (Leopardus pardalis) and margay (L. wiedii), and pampas cat (Lynchailurus colocolo) and most of the tigrina samples (Leopardus tigrina). Within the domestic cat lineage, domestic cat (Felis catus), European wild cat (F. silvestris), and African wild cat (F. libyca) formed a monophyletic trichotomy, which was joined with sand cat (F. margarita) to a common ancestor. Jungle cat (F. chaus) and black-footed cat (F. nigripes) mtDNAs diverged earlier than those of the other domestic cat lineage species and are less closely related. Within the pantherine lineage, phylogenetic analysis identified two distinct groups, uniting lion (P. leo) with leopard (P. pardus) and tiger (P. tigris) with snow leopard (P. uncia).
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Notes Document Type: English Call Number: QL708.5 J68 Approved no
Call Number SLN @ rana @ 276 Serial 501
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Author Joslin, J.O.; Garner, M.; Collins, D.; Kamaka, E.; Sinabaldi, K.; Meleo, K.; Montali, R.; Sundberg, J.; Jenson, A.B.; Ghim, S.; Davidow, B.; Hargis, A.; West, K.; Clark, T.; Haines, D.
Title Viral papilloma and squamous cell carcinomas in snow leopards (Uncia uncia) Type Conference Article
Year 2000 Publication 2000 Proceedings AAZV & IAAAM Joint Conference Abbreviated Journal
Volume Issue Pages 155-158
Keywords captive; snow leopard; squamous cell carcinoma; Uncia uncia; viral papilloma
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Series Editor Series Title Abbreviated Series Title AAZV & IAAAM Joint Conference
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Notes Approved no
Call Number SLN @ rana @ 932 Serial 506
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Author Kazensky, C.A.; Munson, L.; Seal, U.S.
Title The effects of melengestrol acetate on the ovaries of captive wild felids Type Journal Article
Year 1998 Publication Journal-of-Zoo-and-Wildlife-Medicine Abbreviated Journal
Volume 29 Issue 1 Pages 1-5
Keywords Mga; zoo; felids; contraception; Melengestrol-acetate; ovaries; snow-leopard; Panthera-uncia; snow leopard; browse; uncia; panthera; melengestrol; acetate; 1260; effects; study; ovarian; Ovary; captive; wild; wild felid; Wild-Felid; species; Follicle; Cats; cat; recent; Ovulation; doses; Dose; action; Progestin; Disease; veterinary
Abstract Melengestrol acetate (MGA) is the most widely used contraceptive in zoo felids, but the mechanism of contraception and the pathologic effects have not been investigated. For this study, the effects of MGA on folliculogenesis were assessed, and the association of MGA with ovarian lesions was evaluated. Comparisons were made among the histopathologic findings in the ovaries from 88 captive wild felids (representing 15 species) divided into three groups: 37 currently contracepted with MGA, eight previously exposed to MGA, and 43 never contracepted. Ninety-one percent of the felids evaluated had tertiary follicles, and no differences were noted between contracepted and uncontracepted cats. Some MGA-contracepted cats also had corpora lutea indicating recent ovulation. These results indicate that folliculogenesis is not suppressed by current doses of MGA and ovulation occurred in some cats. Therefore, the contraceptive actions of MGA do not occur by suppressing folliculogenesis, and MGA-contracepted felids likely have endogenous estrogens that may confound progestin effects on the uterus. Cystic rete ovarii was the most common pathologic finding, but they were not more prevalent in MGA-contracepted cats. These findings indicate that MGA is not associated with ovarian disease, including ovarian cancer, in contrast to the uterine lesions noted in MGA-treated cats.
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Notes Document Type: English. Affiliation: Taylor Veterinary Hospital, Turlock , California 95382, USA Approved no
Call Number SLN @ rana @ 348 Serial 527
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Author Khan, A.
Title Snow Leopard Occurrence in Mankial Valley, Swat: Final report Type Report
Year 2004 Publication Abbreviated Journal
Volume Issue Pages 1-17
Keywords snow; snow leopard; snow-leopard; leopard; valley; Report; project; International; international snow leopard trust; International-Snow-Leopard-Trust; trust; program; ecosystem; habitat; species; plants; plant; Animals; Animal; birds; research; action; study; survey; Support; Islt; community; Organization; surveys; winter; information; local; sign; pugmarks; feces; scrapes; scrape; prey; prey species; prey-species; recent; population; markhor; hunting; Culture; Pressure; areas; area; feeding; livestock; burning; decline
Abstract Mankial is a sub-valley of the Swat Kohistan. Temperate ecosystem of the valley is intact to a greater extent, which provides habitat to a variety of species of plants, animals and birds. Snow leopard is reported from the valley. To confirm its occurrence, the HUJRA (Holistic Understanding for Justified Research and Action), conducted the study titled “Snow Leopard Survey in Mankial Valley, district Swat, NWFP”. The author provided technical support, while ISLT (The International Snow Leopard Trust) funded the project under its small grants program. The World Wide Fund for Nature-Pakistan (WWF-Pakistan) and the Mankial Community Organization (MCO) facilitated surveys under the project. Surveys revealed that Snow leopard visits parts of the Mankial valley in winter months. Information from the local community shows that Snow leopard remains in the Serai (an off-shoot of the Mankial Valley) from early winter to early spring. Intensive surveys of the prime snow leopard winter habitat in the valley found several snow leopard signs including pugmarks, feces, and scrapes. The study also found occurrence of prey species through indirect evidence though. However, information from the local community confirmed that in the recent past there was a good population of markhor in the valley, which is now reduced to less than 50, mostly due to hunting and habitat disturbance. Hunting is part of the local culture and lifestyle. During winter months hunting pressure is low, as most of the local community migrates to warmer plain areas than Mankial Valley. However, those who live in the area lop oak branches for feeding their livestock and cut trees for burning, in addition to hunting prey species of snow leopard. This has resulted in stunted oak vegetation in most of the lower reaches of the valley and decline of the markhor population.
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Notes Project funded by International Snow Leopard Trust Small Grants Program, 2003. Approved no
Call Number SLN @ rana @ 1069 Serial 530
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Author Khan, A.A.
Title Strategic plan for the conservation of the snow leopard in Pakistan Type Report
Year 2001 Publication Abbreviated Journal
Volume Issue Pages 1-17
Keywords plan; conservation; snow; snow leopard; snow-leopard; leopard; Pakistan; government; Wwf; International; international snow leopard trust; International-Snow-Leopard-Trust; trust
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Publisher WWF Pakistan & International Snow Leopard Trust Place of Publication Pakistan Editor
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Notes Draft – approved by stakeholders & submitted to government. WWF Pakistan & International Snow Leopard Trust. Approved no
Call Number SLN @ rana @ 1003 Serial 531
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