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Author (down) Waits, L.P.; Buckley-Beason, V.A.; Johnson, W.E.; Onorato, D.; McCarthy, T.
Title A select panel of polymorphic microsatellite loci for individual identification of snow leopards (Panthera uncia)  Type Miscellaneous
Year 2006 Publication Molecular Ecology Notes Abbreviated Journal
Volume 7 Issue Pages 311-314
Keywords identification; leopard; leopards; microsatellites,noninvasive genetic sampling,Panthera uncia,snow leopard; panthera; Panthera-uncia; panthera uncia; snow; snow-leopard; snow-leopards; snow leopard; snow leopards; uncia; endangered; carnivores; carnivore; mountain; region; central; Central Asia; asia; methods; conservation; management; Molecular; individual identification; Hair; domestic; cat; felis; captive; number; probability; using; wild
Abstract Snow leopards (Panthera uncia) are elusive endangered carnivores found in remote mountain regions of Central Asia. New methods for identifying and counting snow leopards are needed for conservation and management efforts. To develop molecular genetic tools for individual identification of hair and faecal samples, we screened 50 microsatellite loci developed for the domestic cat (Felis catus) in 19 captive snow leopards. Forty-eight loci were polymorphic with numbers of alleles per locus ranging from two to 11. The probability of observing matching genotypes for unrelated individuals (2.1 x10-11) and siblings (7.5x10-5) using the 10 most polymorphic loci was low, suggesting that this panel would easily discriminate among individuals in the wild.
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Call Number SLN @ rana @ 865 Serial 1001
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Author (down) Vereschagin N.K., S.T.B.
Title Rare mammals in the USSR: protection challenges Type Miscellaneous
Year 1976 Publication Abbreviated Journal
Volume Issue Pages 3-9
Keywords Ussr; mammals; game species; non-game species; rare species; vanishing species; IUCN Red Data Book; snow leopard.; 8480; Russian
Abstract A group of rare and endangered species was segregated within the game and non-game mammals of the USSR. Some species in the group were formerly referred to game species. But due to over-hunting and the absence of measures aimed at their reproduction the population dropped sharply. Mammal fauna of the USSR includes more than 80 species that require special protection. The Red list of IUCN includes, among the others, white bear, Transcaucasian sub-species of brown bear, Amur and Turan tigers, snow leopard, Caucasian and Amur leopards, caracal, cheetah, Tien-Shan and Ussuri sub-species of dhole, Atlantic walrus, island seal, kulan, Bukhara red deer, New Land reindeer, goitered gazelle, Menzbier's marmot.
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Notes Full text available in RussianJournal Title: Rare mammals of USSR fauna. Approved no
Call Number SLN @ rana @ 825 Serial 985
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Author (down) Swanson, W.F.
Title Research in Nondomestic Species: Experiences in Reproductive Physiology Research for Conservation of Endangered Felids Type Miscellaneous
Year 2003 Publication ILAR Journal Abbreviated Journal
Volume 4 Issue Pages 307-316
Keywords artificial insemination; capacity building; catmodels; cryopreservation; electroejaculation; embryo transfer; mobile laboratory; nondomestic felids
Abstract Tremendous strides have been made in recent years to broaden our understanding of reproductive processes in nondomestic felid species and further our capacity to use this basic knowledge to control and manipulate reproduction of endangered cats. Much of that progress has culminated from detailed scientific studies conducted in nontraditional laboratory settings, frequently at collaborating zoological parks but also under more primitive conditions, including in the field. A mobile laboratory approach is described, which incorporates a diverse array of disciplines and research techniques. This approach has been extremely useful, especially for conducting gamete characterization and function studies as well as reproductive surveys, and for facilitating the development of assisted reproductive technology. With continuing advances in assisted reproduction in rare felids, more procedures are being conducted primarily as service-related activities, targeted to increase effectiveness of species propagation and population management. It can be a challenge for both investigators and institutional animal care and use committees (IACUCs) to differentiate these service-based procedures from traditional research studies (that require IACUC oversight). For research with rare cat species, multi-institutional collaboration frequently is necessary to gain access to scientifically meaningful numbers of study subjects. Similarly, for service-based efforts, the ability to perform reproductive procedures across institutions under nonstandard laboratory conditions is critical to applying reproductive sciences for managing and preserving threatened cat populations. Reproductive sciences can most effectively assist population management programs (e.g., Species Survival Plans) in addressing conservation priorities if these research and service- related procedures can be conducted “on the road” at distant national and international locales. This mobile laboratory approach has applications beyond endangered species research, notably for other scientific fields (e.g., studies of hereditary disease in domestic cat models) in which bringing the laboratory to the subject is of value.
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Call Number SLN @ rana @ 901 Serial 953
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Author (down) Rothschild, B.M.; Rothschild, C.; Woods, R.J.
Title Inflammatory arthritis in large cats: An expanded spectrum of spondyloarthropathy Type Journal Article
Year 1998 Publication Journal of Zoo and Wildlife Medicine Abbreviated Journal
Volume 29 Issue 3 Pages 279-284
Keywords Acinonyx-jubatus; cheetah; Felidae; Felis-concolor; mountain-lion; Felis-pardalis; ocelot; Panthera-leo; African-lion; Panthera-onca; jaguar; Panthera-pardus; leopard; Panthera-tigris; tiger; Panthera-uncia; snow-leopard; snow leopard; Acinonyx; jubatus; felis; concolor; mountain; lion; pardalis; panthera; leo; african; onca; pardus; tigris; uncia; browse; 1240
Abstract Spondyloarthropathy was documented for the first time in 14 (3.7%) of 386 large cats, affecting eight species belonging to three genera. The limited distribution of joint erosions, associated with spine and sacroiliac joint pathology, was indistinguishable from that occurring in humans with spondyloarthropathy of the reactive type. This form of inflammatory arthritis is almost twice as common as osteoarthritis (for felids as a whole), and animal well-being may be enhanced by its recognition and by initiation of specific treatment.
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Notes Document Type: English Approved no
Call Number SLN @ rana @ 353 Serial 832
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Author (down) Roth, T.L.; Howard, J.G.; Donoghue, A.M.; Swanson, W.F.; Wildt, D.E.
Title Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro Type Journal Article
Year 1994 Publication J Reprod Fertil Abbreviated Journal
Volume 101 Issue 3 Pages 563-569
Keywords Animal; Carnivora; physiology; Cell; survival; Cells; Cultured; Comparative; study; Culture; Media; Female; Male; Pentoxifylline; Sperm; Motility; Ovum; Interactions; Spermatozoa; cytology; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 190
Abstract Electroejaculates from eight snow leopards were used to determine how the motility of spermatozoa was influenced by (i) type of media (Ham's F10, PBS, human tubal fluid or RPMI-1640); (ii) holding temperature (23 degrees C versus 37 degrees C); (iii) washing of spermatozoa and (iv) a sperm metabolic enhancer, pentoxifylline. The duration of sperm motility was assessed by evaluating samples in each treatment every hour for 6 h and a sperm motility index (a value combining percentage sperm motility and rate of forward progression) calculated. Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. During the 6 h co-incubation, the sperm motility index in PBS with pentoxifylline was greater (P < 0.05) than in PBS alone which, in turn, was greater (P < 0.05) than in the other three test media. Washing the spermatozoa enhanced (P < 0.05) motility in both PBS and PBS plus pentoxifylline relative to unwashed samples, but there was no effect (P > 0.05) of holding temperature. Pentoxifylline supplementation enhanced (P < 0.05) the proportion of cat eggs with bound, but not penetrated, snow leopard spermatozoa in the inner layer of the zona pellucida, and there were no spermatozoa in the perivitelline space.(ABSTRACT TRUNCATED AT 250 WORDS)
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ISSN 0022-4251 ISBN Medium
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Notes Document Type: eng Approved no
Call Number SLN @ rana @ 253 Serial 828
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Author (down) Roth, T.L.; Armstrong, D.L.; Barrie, M.T.; Wildt, D.E.
Title Seasonal effects on ovarian responsiveness to exogenous gonadotrophins and successful artificial insemination in the snow leopard (Uncia uncia) Type Journal Article
Year 1997 Publication Reprod Fertil Dev Abbreviated Journal
Volume 9 Issue 3 Pages 285-295
Keywords Animal; Carnivora; anatomy; histology; Blood; physiology; Estradiol; Female; Gonadotropins; administration; dosage; pharmacology; Chorionic; Equine; Human; Insemination; artificial; methods; veterinary; Laparoscopy; Male; Oocytes; cytology; Ovary; drug; effects; Ovulation; Induction; Pregnancy; Progesterone; Seasons; Support; Non-U.S.Gov't; browse; non; us; gov't; government; 400
Abstract Ovaries of the seasonally-breeding snow leopard (Uncia uncia) were examined to determine whether they were responsive to exogenous gonadotrophins throughout the year. The potential of laparoscopic artificial insemination (AI) also was assessed for producing offspring. During the non-breeding, pre-breeding, breeding and post-breeding seasons, females (n = 20) were treated with a standardized, dual- hormone regimen given intramuscularly (600 I.U. of equine chorionic gonadotrophin followed 80-84 h later with 300 I.U. of human chorionic gonadotrophin (hCG)). Laparoscopy was performed 45-50 h after administration of hCG, and all ovarian structures were described. Females with fresh corpora lutea (CL) were inseminated, and anovulatory females were subjected to follicular aspiration to examine oocyte quality. Snow leopards responded to exogenous gonadotrophins throughout the year. Mean number of total ovarian structures (distinct follicles mature in appearance plus CL) did not differ (P > or = 0.05) with season, but the proportion of CL: total ovarian structures was greater (P < 0.01) for the breeding season compared with all other seasons. The proportion of females ovulating was greater (P < 0.05) during the breeding and post-breeding seasons than during the pre-breeding and non- breeding seasons respectively. No Grade-1 quality oocytes were recovered from follicles of anovulatory females. Serum concentrations of oestradiol-17 beta appeared elevated in all females, and neither oestradiol-17 beta concentrations nor progesterone concentrations differed (P > or = 0.05) among seasons. Of 15 females artificially inseminated, the only one that was inseminated in the non-breeding season became pregnant and delivered a single cub. This is the first successful pregnancy resulting from AI in this endangered species.
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ISSN 1031-3613 ISBN Medium
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Notes Document Type: eng Approved no
Call Number SLN @ rana @ 325 Serial 830
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Author (down) Rodgers, T. W.,Janecka, J. E.
Title Applications and techniques for non-invasive faecal genetics research in felid conservation Type Journal Article
Year 2013 Publication Abbreviated Journal
Volume Issue Pages
Keywords Conservation . Elusive species . Faecal DNA . Felidae . Non-invasive genetics
Abstract Non-invasive genetic techniques utilising DNA extracted from faeces hold great promise for felid conservation research. These methods can be used to establish species

distributions, model habitat requirements, analyse diet, estimate abundance and population density, and form the basis for population, landscape and conservation genetic analyses. Due to the elusive nature of most felid species, non-invasive genetic methods have the potential to provide

valuable data that cannot be obtained with traditional observational or capture techniques. Thus, these methods are particularly valuable for research and conservation of endangered

felid species. Here, we review recent studies that use non-invasive faecal genetic techniques to survey or study wild felids; provide an overview of field, laboratory and analysis techniques; and offer suggestions on how future non-invasive genetic studies can be expanded or improved to more effectively support conservation.
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Call Number SLN @ rakhee @ Serial 1430
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Author (down) Rode, J., Pelletier, A., Fumey, J., Rode, S., Cabanat, A. L., Ouvrard, A., Chaix, B., White, B., Harnden, M., Xuan, N. T., Vereshagin, A., Casane, D.
Title Diachronic monitoring of snow leopards at Sarychat-Ertash State Reserve (Kyrgyzstan) through scat genotyping: a pilot study Type Journal Article
Year 2020 Publication bioRxiv Abbreviated Journal
Volume Issue Pages 1-21
Keywords snow leopard, noninvasive genotyping, population dynamics, microsatellite, relatedness, diachronic monitoring, citizen science, Central Asia
Abstract Snow leopards (Panthera uncia) are a keystone species of Central Asia’s high mountain ecosystem. The species is listed as vulnerable and is elusive, preventing accurate population assessments that could inform conservation actions. Non-invasive genetic monitoring conducted by citizen scientists offers avenues to provide key data on this species that would otherwise be inaccessible. From 2011 to 2015, OSI-Panthera citizen science expeditions tracked signs of presence of snow leopards along transects in the main valleys and crests of the Sarychat-Ertash State Reserve (Kyrgyzstan). Scat samples were genotyped at seven autosomal microsatellite loci and at a X/Y locus for sex identification, which allowed estimating a minimum of 11 individuals present in the reserve from 2011 to 2015. The genetic recapture of 7 of these individuals enabled diachronic monitoring, providing indications of individuals’ movements throughout the reserve. We found putative family relationships between several individuals. Our results demonstrate the potential of this citizen science program to get a precise description of a snow leopard population through time.
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Notes Approved no
Call Number Serial 1602
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Author (down) Pollock, R.V.; Carmichael, L.E.
Title Use of modified live feline panleukopenia virus vaccine to immunize dogs against canine parvovirus Type Journal Article
Year 1983 Publication Am J Vet Res Abbreviated Journal
Volume 44 Issue 2 Pages 169-175
Keywords Animal; Antibodies; Viral; biosynthesis; Dog; Diseases; microbiology; prevention; control; Dogs; Dose; Response; Relationship; Immunologic; Parvoviridae; immunology; Parvovirus; Feline; growth; development; Support; Non-U.S.Gov't; Vaccines; Attenuated; Virus; veterinary; Replication; browse; 350
Abstract Modified live feline panleukopenia virus (FPLV) vaccine protected dogs against canine parvovirus (CPV) infection. However, unlike the long- lived (greater than or equal to 20-month) immunity engendered by CPV infection, the response of dogs to living FPLV was variable. Doses of FPLV (snow leopard strain) in excess of 10(5.7) TCID50 were necessary for uniform immunization; smaller inocula resulted in decreased success rates. The duration of immunity, as measured by the persistence of hemagglutination-inhibiting antibody, was related to the magnitude of the initial response to vaccination; dogs with vigorous initial responses resisted oronasal CPV challenge exposure 6 months after vaccination, and hemagglutination-inhibiting antibodies persisted in such dogs for greater than 1 year. Limited replication of FPLV in dogs was demonstrated, but unlike CPV, the feline virus did not spread to contact dogs or cats. Adverse reactions were not associated with living FPLV vaccination, and FPLV did not interfere with simultaneous response to attenuated canine distemper virus.
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ISSN 0002-9645 ISBN Medium
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Notes Document Type: eng Approved no
Call Number SLN @ rana @ 69 Serial 784
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Author (down) Pavlinov I.Ya.
Title Mammals of Eurasia. II. Non-Rodentia: Taxonomic and geographic reference book Type Miscellaneous
Year 1995 Publication Abbreviated Journal
Volume Issue Pages 167-168
Keywords Ussr; taxonomy; synonyms; distribution; snow leopard.; 7840; Russian
Abstract This reference book provides a detailed epi-species classification of terrain mammal orders other than rodents. Identification keys for taxons, information about geographical distribution, synonyms, and comments on taxonomy are given. Genus Uncia Gray, 1854 includes one species Uncia uncia (Schreber, 1776), distributed in highlands of Central Asia (Tibet, Pamir, Tien-Shan, Altai). Synonyms: irbis Ehrenberg, 1830; uncioides Horsfield, 1855; schneideri Zukovsky, 1950.
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Notes Full text available in Russian Approved no
Call Number SLN @ rana @ 762 Serial 761
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