| Records |
| Author |
Trepanier, L.A.; Cribb, A.E.; Spielberg, S.P.; Ray, K. |
| Title |
Deficiency of cytosolic arylamine N-acetylation in the domestic cat and wild felids caused by the presence of a single NAT1-like gene |
Type |
Journal Article |
| Year |
1998 |
Publication |
Pharmacogenetics |
Abbreviated Journal |
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| Volume |
8 |
Issue |
2 |
Pages |
169-179 |
| Keywords |
Acetylation; Amino; Acid; Sequence; Animal; Arylamine; N-Acetyltransferase; metabolism; Base; Blotting; Southern; Carnivora; genetics; Cats; Cytosol; enzymology; Dna; Human; Isoenzymes; Liver; Molecular; Data; Polymerase; Chain; Reaction; Rabbits; Homology; Nucleic Acid; Substrate; Specificity; Support; U.S.Gov't; P.H.S.; browse; nucleic; us; government; 130 |
| Abstract |
The purpose of this study was to determine the molecular basis for a relative deficiency in the cat of cytosolic arylamine N- acetyltransferase (NAT), an enzyme family that is important in the metabolism of xenobiotics and that normally consists of at least two related enzymes, NAT1 and NAT2. N-acetyltransferase in feline liver showed high affinity (mean Km = 2.1 microM) for p-aminobenzoic acid, an NAT1 selective substrate in humans and rabbits, but showed a very poor affinity (mean Km > 10 mM) for sulfamethazine, an NAT2 selective substrate in humans and rabbits. Immunoreactive N-acetyltransferase was detected in feline liver, bladder and colon using an NAT1-specific antipeptide antibody, but was not detected in any tissues using an NAT2- specific antibody. Southern blot analysis of genomic DNA demonstrated a single band in domestic cats using each of six restriction digests; single bands were also found on Southern blot analysis of six wild felids. The deduced amino acid sequence of the central portion of feline N-acetyltransferase, obtained by polymerase chain reaction amplification in both domestic cats and seven wild felids (lion, tiger, lynx, snow leopard, bobcat, Asian leopard cat and cheetah), contained three residues, Phe125, Arg127, and Tyr129, which determine NAT1-like substrate specificity in humans. These results support the conclusion that cytosolic arylamine N-acetylation activity is low in the cat because of the presence of a single N-acetyltransferase that has substrate specificity, immunogenicity and sequence characteristics similar to human NAT1, and that the unusual presence of only a single N- acetyltransferase gene appears to be a family wide trait shared by other felids. |
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0960-314x |
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Document Type: eng |
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no |
| Call Number |
SLN @ rana @ 345 |
Serial |
968 |
| Permanent link to this record |
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| Author |
Wingard, J.R.; Zahler, P. |
| Title |
Silent Steppe: The Illegal Wildlife Trade Crisis in Mongolia |
Type |
Report |
| Year |
2006 |
Publication |
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Abbreviated Journal |
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| Volume |
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Issue |
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Pages |
1-170 |
| Keywords |
steppe; illegal; wildlife; trade; Mongolia; study; threat; populations; population; areas; area; fur; fur trade; fur-trade; game; meat; hunting; Chain; impact; biodiversity; Biodiversity conservation; conservation; rural; livelihood; Wildlife-Management; management; survey; survey methods; methods; history; action; International; enforcement; domestic; community-based; approach |
| Abstract |
The current study in Mongolia is truly groundbreaking, in that it shows that the problem of commercial wildlife trade is also vast, unsustainable, and a major threat to wildlife populations in other areas. This paper's Executive Summary briefs the topics of wildlife trade in Mongolia, fur trade, medicinal trade, game meat trade, trophy and sport hunting, trade chains and markets, trade sustainability, impacts of wildlife trade on biodiversity conservation, impacts of trade on rural livelihoods, enabling wildlife management, and management recommendations. The main content of the paper includes: wildlife trade survey methods, a history of wildlife trade in Mongolia, wildlife take and trade today, enabling wildlife management, and recommendations and priority actions. The recommendations have been divided into six separate sections, including (1) cross-cutting recommendations, (2) international trade enforcement, (3) domestic trade enforcement, (4) hunting management, (5) trophy and sport hunting management, and (6) community-based approaches. Each section identifies short-term, long-term, and regulatory goals in order of priority within each subsection. |
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World Bank |
Place of Publication |
Washington, D.C. |
Editor |
East Asia and Pacific Environment and Social Development Department |
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| Notes |
Mongolian version. English language translation is also available in the SLN bibliography. Mongolia Discussion Papers. East Asia and Pacific Environment and Social Development Department. Washington D.C.: World Bank. |
Approved |
no |
| Call Number |
SLN @ rana @ 1079 |
Serial |
1026 |
| Permanent link to this record |
