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Author (up) Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. url  openurl
  Title Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces Type Journal Article
  Year 1994 Publication Biol Reprod Abbreviated Journal  
  Volume 51 Issue 4 Pages 776-786  
  Keywords Animal; Carbon; Radioisotopes; Carnivora; Cats; Chromatography; High; Pressure; Liquid; Comparative Study; Estradiol; metabolism; Estrone; feces; chemistry; Female; Ovary; physiology; Pregnancy; Progesterone; Pseudopregnancy; Support; Non-U.S.Gov't; browse; non; government; gov't; us; 170  
  Abstract Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species.  
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  ISSN 0006-3363 ISBN Medium  
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  Notes Document Type: eng Approved no  
  Call Number SLN @ rana @ 251 Serial 198  
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Author (up) Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. url  openurl
  Title Steroid Metabolism and the Effectiveness of Fecal Assays for Assessing Reproductive Status in Felids Type Journal Article
  Year 1994 Publication Biology of Reproduction Abbreviated Journal  
  Volume 50 Issue suppl 1 Pages 185  
  Keywords fecal-analysis; breeding; reproduction; medicine; medical; zoo; zoos; veterinary; snow-leopard; snow leopard; browse; fecal; analysis; 1420  
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  Notes Twenty-seventh Annual Meeting of the Society for the Study of Reproduction, Ann Arbor, Michigan, USA, July 24-27, 1994 Call Number: QP251 .B56 Approved no  
  Call Number SLN @ rana @ 203 Serial 197  
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Author (up) Graham, L.H.; Goodrowe, K.L.; Raeside, J.I.; Liptrap, R.M. url  openurl
  Title Non-invasive monitoring of ovarian function in several felid species by measurement of fecal estradiol-17-beta and progestins Type Journal Article
  Year 1995 Publication Zoo Biology Abbreviated Journal  
  Volume 14 Issue 3 Pages 223-237  
  Keywords Artificial-Breeding-Program; captive-management; Estradiol-17beta; Pregnancy; Progesterone; Progestin; sexual-behavior; genetics; zoo; medicine; veterinary; snow-leopard; feces; fecal-analysis; snow leopard; artificial; breeding; program; captive; management; Estradiol; 17beta; sexual; behavior; browse; snow; leopard; fecal; analysis; 1390  
  Abstract An extraction and assay procedure to measure fecal estradiol-17-beta and progestin concentrations in several cat species was developed and validated for use for noninvasive monitoring of ovarian function. Fecal samples were collected over a range of 3-20 months from female tigers (three), lions (three), snow leopards (three), cheetahs (two), caracals (two), and domestic cats (five). Samples were extracted with 90% methanol, lipids removed with petroleum ether, and the estradiol and progestins in the methanol measured by radioimmunoassay (RIA). High Performance Liquid Chromatography (HPLC) fractionation and subsequent RIA of the fractions indicated that the estradiol-17-beta antiserum cross-reacted primarily with estradiol-17-beta in the feces of lions and tigers and was assumed to be specific for estradiol-17-beta in the feces of other species as well. However, there were several immunoreactive compounds, presumably progesterone metabolites, excreted in the feces which varied both quantitatively and qualitatively among species. The behavior of tigers, lions, cheetahs, and caracals was visually monitored during the collection period and frequency of sexual behaviors was positively correlated with increases in fecal estradiol in all species observed. The mean fecal estradiol-17-beta peaks were as follows: tigers, 128.0 +- 13.1; lions, 186.0 +- 14.8; snow leopards, 136.7 +- 15.9; cheetahs, 140.9 +- 9.0; caracals, 24.5 +- 4.0; and domestic cats 158.9 +- 19.3 ng/gm. Fecal progestin concentrations rose significantly (P lt 0,001) only after breeding or during pregnancy and were as follows: tigers, 5.6 +- 0.6; lions, 1.9 +- 0.1; cheetahs, 8.4 +- 1.1; and caracals, 2.4 +- 0.4 mu-g/gm. Fecal progestins were elevated for one-half to two-thirds of the gestation length during presumed pseudopregnancy but remained elevated throughout successful pregnancies. These results suggest that ovarian function can be monitored noninvasively in the family Felidae by the measurement of fecal estradiol-17-beta and progestin concentrations.  
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  Notes Document Type: English Approved no  
  Call Number SLN @ rana @ 279 Serial 345  
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