Abstract: Accumulation of carotenoids varies greatly among animal species and is not fully characterized.
Circulating carotenoid concentration data in captive wild animals are limited and may be useful for their management.
Serum carotenoid concentrations and dietary intakes were surveyed and the extent of accumulation
categorized for 76 species of captive wild animals at Brookfield Zoo. Blood samples were obtained opportunistically
from 275 individual animals immobilized for a variety of reasons; serum was analyzed for a- and b-carotene,
lutein 1 zeaxanthin, lycopene, b-cryptoxanthin and canthaxanthin. Total carotenoid content of diets was calculated
from tables and chemical analyses of commonly consumed dietary components. Diets were categorized as
low, moderate or high in carotenoid content as were total serum carotenoid concentrations. Animals were
classified as unknown, high, moderate or low (non-) accumulators of dietary cartenoids. Nonaccumulators had total
serum carotenoid concentrations of 0-101 nmol/L, whereas accumulators had concentrations that ranged widely,
from 225 to 35,351 nmol/L. Primates were uniquely distinguished by the widest range of type and concentration
of carotenoids in their sera. Most were classified as high to moderate accumulators. Felids had high accumulation
of b-carotene regardless of dietary intake, whereas a wide range of exotic birds accumulated only the xanthophylls,
lutein 1 zeaxanthin, canthaxanthin or cryptoxanthin. The exotic ungulates, with the exception of the bovids, had
negligible or nondetectable carotenoid serum concentrations despite moderate intakes. Bovids accumulated only
b-carotene despite moderately high lutein 1 zeaxanthin intakes. Wild captive species demonstrated a wide variety
of carotenoid accumulation patterns, which could be exploited to answer remaining questions concerning carotenoid
metabolism and function.

Abstract: The snow leopard has never been common, but there may be fewer than 4,000 left in its Himalayan habitat, and poaching and tourism-related development in the region could drive its numbers down further.

Abstract: The author offers advice on how a captive-raised snow leopard cub could be acclimated to humans so it could be used as a zoo “ambassador”. The cub had negative experiences with humans and lacked socialization with other animals and conspecifics. Methods of avoiding and redirecting the cub's aggressive behavior are suggested. lgh.

Abstract: Melengestrol acetate (MGA) is the most widely used contraceptive in zoo felids, but the mechanism of contraception and the pathologic effects have not been investigated. For this study, the effects of MGA on folliculogenesis were assessed, and the association of MGA with ovarian lesions was evaluated. Comparisons were made among the histopathologic findings in the ovaries from 88 captive wild felids (representing 15 species) divided into three groups: 37 currently contracepted with MGA, eight previously exposed to MGA, and 43 never contracepted. Ninety-one percent of the felids evaluated had tertiary follicles, and no differences were noted between contracepted and uncontracepted cats. Some MGA-contracepted cats also had corpora lutea indicating recent ovulation. These results indicate that folliculogenesis is not suppressed by current doses of MGA and ovulation occurred in some cats. Therefore, the contraceptive actions of MGA do not occur by suppressing folliculogenesis, and MGA-contracepted felids likely have endogenous estrogens that may confound progestin effects on the uterus. Cystic rete ovarii was the most common pathologic finding, but they were not more prevalent in MGA-contracepted cats. These findings indicate that MGA is not associated with ovarian disease, including ovarian cancer, in contrast to the uterine lesions noted in MGA-treated cats.

Abstract: A high percentage of rare species in Central Asia experience limited conditions for distribution. Geographic centers with higher species diversity are generally constrained in terms of territory: they are formed when ranges overlap. But in Central Asia and along its borders with Russia, centers of biodiversity overlap at the very marginal edges of ranges. Central Asian species cross into Russian territory, where desert and steppe are replaced by thick forest. Here the northern borders of their ranges are sharply fragmented and isolated. Typical examples for Central Asia are the ranges of the cheetah (Acinonyx jubatus), Asian leopard (Panthera pardus caucasica), striped hyena (Hyaena hyaena), Bukhara deer (Census elaphus bactrianus), markhor (Capra falconeri), blue sheep (Pseudois nayauf) and argali (Ovis ammon). In Russia are the Altai subspecies of argali, the Siberian argali (O.a.ammon), the mountain goat (Capra sibirica), Mongolian gazelle (Procapra gutturosa), snow leopard (Uncia uncia), Pallas' cat (Felis manul), dhole (Cuon alpinus), grey marmot (Marmota baibacina), Mongolian marmot (M. sibirica) and tolai hare (Lepus tolai). Where the numbers o f individuals has fallen to extreme lows, the most effective mechanism for species survival may be supporting the integrity of ranges, in order to preserve population exchanges between neighboring groups. The geographic location of reserves and other protected territories is vitally important for the survival of Central Asian species, given the acute fragmentation of their ranges. These reserves should include significant, viable centers of population the key places. Wherever the creation of permanent protected territories is impossible, a new tactic must be found, such as introducing temporary limitations on the use of land for agriculture and hunting. But all protected territories, whether temporary or permanent, should be connected, forming a core and periphery. The marginal range areas must not be forgotten, if total protection of endangered populations is to be accomplished.