Abstract: An extraction and assay procedure to measure fecal estradiol-17-beta and progestin concentrations in several cat species was developed and validated for use for noninvasive monitoring of ovarian function. Fecal samples were collected over a range of 3-20 months from female tigers (three), lions (three), snow leopards (three), cheetahs (two), caracals (two), and domestic cats (five). Samples were extracted with 90% methanol, lipids removed with petroleum ether, and the estradiol and progestins in the methanol measured by radioimmunoassay (RIA). High Performance Liquid Chromatography (HPLC) fractionation and subsequent RIA of the fractions indicated that the estradiol-17-beta antiserum cross-reacted primarily with estradiol-17-beta in the feces of lions and tigers and was assumed to be specific for estradiol-17-beta in the feces of other species as well. However, there were several immunoreactive compounds, presumably progesterone metabolites, excreted in the feces which varied both quantitatively and qualitatively among species. The behavior of tigers, lions, cheetahs, and caracals was visually monitored during the collection period and frequency of sexual behaviors was positively correlated with increases in fecal estradiol in all species observed. The mean fecal estradiol-17-beta peaks were as follows: tigers, 128.0 +- 13.1; lions, 186.0 +- 14.8; snow leopards, 136.7 +- 15.9; cheetahs, 140.9 +- 9.0; caracals, 24.5 +- 4.0; and domestic cats 158.9 +- 19.3 ng/gm. Fecal progestin concentrations rose significantly (P lt 0,001) only after breeding or during pregnancy and were as follows: tigers, 5.6 +- 0.6; lions, 1.9 +- 0.1; cheetahs, 8.4 +- 1.1; and caracals, 2.4 +- 0.4 mu-g/gm. Fecal progestins were elevated for one-half to two-thirds of the gestation length during presumed pseudopregnancy but remained elevated throughout successful pregnancies. These results suggest that ovarian function can be monitored noninvasively in the family Felidae by the measurement of fecal estradiol-17-beta and progestin concentrations.

Abstract: Snow leopards are to be found in the most extreme eastern, southeastern and southern mountainous regions, including the Altai. Saur, Tarbagatai. Dzhungarian, Alatau, Northern and Western Tian-Shan ranges. The snow leopard or irbis is the most rare in eastern Kazakstan in the ranges of Katunskie Belki, South Altai, Kurchumski, Sarymsakty, Saur and Tarbagatai. Total snow leopard population in Kazakstan is estimated at no more than 100-110 animals, including 20-25 in the central part of the Zailisky-Alatau. Although there are nine protected areas in Kazakstan, snow leopards are only regularly reported
from the Aksu-Dzhabagly and Almaty reserves and occasionally in Markakolsky Reserve. The major threats to the species include: Deliberate poaching with the aim of selling the valuable fur of the snow leopard; habitat loss resulting from the expansion of human activity in its mountain habitat, and deliberate or retaliatory killing by shepherds in response to predation upon livestock.

Abstract: This reference book provides a detailed epi-species classification of terrain mammal orders other than rodents. Identification keys for taxons, information about geographical distribution, synonyms, and comments on taxonomy are given. Genus Uncia Gray, 1854 includes one species Uncia uncia (Schreber, 1776), distributed in highlands of Central Asia (Tibet, Pamir, Tien-Shan, Altai). Synonyms: irbis Ehrenberg, 1830; uncioides Horsfield, 1855; schneideri Zukovsky, 1950.

Abstract: A 5-mo-old male snow leopard (Panthera uncia) was presented for mild rear leg lameness. Osteomyelitis was suspected on the basis of radiographic changes and confirmed by histopathology of a biopsy sample from the affected bone. Aerobic cultures of the biopsies repeatedly grew Klebsiella oxytoca. Repeated anaerobic and fungal cultures did not result in growth. The leopard was treated unsuccessfully with cefadroxil, chloramphenicol, and trimethoprim/sulfadiazine despite apparent in vitro sensitivity to these antibiotics. Successful resolution was eventually achieved with enrofloxacin, 7.5 mg/kg p.o. b.i.d. for 60 days. The number of bones involved (right humerus, right and left ulna, right and left radius, right and left femur, right and left tibia, mandible, right metatarsus) made this an unusual presentation of osteomyelitis.