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Hochstrasser, K., Wachter, E., Reisinger, P. W., Greim, M., Albrecht, G. J., & Gebhard, W. (1993). Amino acid sequences of mammalian kazal-type proteinase inhibitors from salivary glands. Comp Biochem Physiol B, 106(1), 103–108.
Abstract: 1. The amino acid sequences of bikazins (the double-headed Kazal-type proteinase inhibitors from submandibular glands) isolated from the snow leopard (Unica unica), the European mink (Mustela lutreola), and the European pine marten (Martes martes) were determined. 2. N-terminal domains of bikazins are characterized by a cysteine residue spacing that differs from that of C-terminal domains of bikazins and other Kazal-type proteinase inhibitor domains. 3. N-terminal sequences of bikazins seem to be specific for, and highly conserved within, each Carnivora family.
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Brown, J. L., Wasser, S. K., Wildt, D. E., & Graham, L. H. (1994). Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces. Biol Reprod, 51(4), 776–786.
Abstract: Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species.
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Jackson, R., Roe, J., Wangchuk, R., & Hunter, D. (2005). Camera-Trapping of Snow Leopards. Cat News, 42(Spring), 19–21.
Abstract: Solitary felids like tigers and snow leopards are notoriously difficult to enumerate, and indirect techniques like pugmark surveys often produce ambiguous information that is difficult to interpret because many factors influence marking behavior and frequency (Ahlborn & Jackson 1988). Considering the snow leopard's rugged habitat, it is not surprising then that information on its current status and occupied range is very limited. We adapted the camera-trapping techniques pioneered by Ullas Karanth and his associates for counting Bengal tigers to the census taking of snow leopards in the Rumbak watershed of the India's Hemis High Altitude National Park (HNP), located in Ladakh near Leh (76ø 50' to 77ø 45' East; 33ø 15' to 34ø 20'North).
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Espinosa-Aviles, D., Taylor, M. L., Del Rocio Reyes-Montes, M., & Pe'rez-Torrez, A. (2008). Molecular findings of disseminated histoplasmosis in two captive snow leopards (Uncia uncia) (Vol. 39).
Abstract: This paper reports two cases of disseminated histoplasmosis in captive snow leopards (Uncia uncia). Histoplasmosis was diagnosed based on histopathology, immunohistochemistry, transmission electron microscopy, and molecular findings.
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Sundberg, J. P., Van Ranst, M., Montali, R., Homer, B. L., Miller, W. H., Rowland, P. H., et al. (2000). Feline papillomas and papillomaviruses. Vet Pathol, 37(1), 1–10.
Abstract: Papillomaviruses (PVs) are highly species- and site-specific pathogens of stratified squamous epithelium. Although PV infections in the various Felidae are rarely reported, we identified productive infections in six cat species. PV-induced proliferative skin or mucous membrane lesions were confirmed by immunohistochemical screening for papillomavirus-specific capsid antigens. Seven monoclonal antibodies, each of which reacts with an immunodominant antigenic determinant of the bovine papillomavirus L1 gene product, revealed that feline PV capsid epitopes were conserved to various degrees. This battery of monoclonal antibodies established differential expression patterns among cutaneous and oral PVs of snow leopards and domestic cats, suggesting that they represent distinct viruses. Clinically, the lesions in all species and anatomic sites were locally extensive and frequently multiple. Histologically, the areas of epidermal hyperplasia were flat with a similarity to benign tumors induced by cutaneotropic, carcinogenic PVs in immunosuppressed human patients. Limited restriction endonuclease analyses of viral genomic DNA confirmed the variability among three viral genomes recovered from available frozen tissue. Because most previous PV isolates have been species specific, these studies suggest that at least eight different cat papillomaviruses infect the oral cavity (tentative designations: Asian lion, Panthera leo, P1PV; snow leopard, Panthera uncia, PuPV-1; bobcat, Felis rufus, FrPV; Florida panther, Felis concolor, FcPV; clouded leopard, Neofelis nebulosa, NnPV; and domestic cat, Felis domesticus, FdPV-2) or skin (domestic cat, F. domesticus, FdPV-1; and snow leopard, P. uncia, PuPV-2).
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Jackson, R. (1999). Snow Leopards, Local People and Livestock Losses: Finding solutions using Appreciative Participatory Planning and Action (APPA) in the Markha Valley of Hemis National Park, Ladakh, October 6-26, 1999. Cat News, 31(Autumn), 22–23.
Abstract: Livestock depredation is emerging as a significant issue across the Himalaya, including the Hemis National Park (HNP) in Ladakh. Some consider that this protected area harbors the best snow leopard population in India, but local herders perceive the endangered snow leopard as a serious threat to their livelihood.
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Gosselin, S. J., Loudy, D. L., Tarr, M. J., Balistreri, W. F., Setchell, K. D., Johnston, J. O., et al. (1988). Veno-occlusive disease of the liver in captive cheetah. Vet Pathol, 25(1), 48–57.
Abstract: Liver tissues from 126 captive cheetah were evaluated by light microscopy and histochemistry; eight animals were evaluated by electron microscopy. The main hepatic lesion, a vascular lesion resembling veno- occlusive disease (VOD) of the liver and characterized by subendothelial fibrosis and proliferation of smooth muscle-like cells in the central veins, was seen in 60% of the sexually mature cheetah. Although this hepatic vascular lesion was seen in cheetah as young as 1 year of age, the most severe lesions, usually associated with liver failure, were found in cheetah between the ages of 6 and 11. There was no sex predisposition, and in approximately 40% of the VOD cases, liver disease was not suspected clinically or at necropsy. VOD was found in other felidae, especially in the snow leopard. High levels of vitamin A in livers, as well as in diets of the cheetah, could be a contributing factor in the development of VOD in some groups of cheetah.
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Fox, J. L. (1997). Conflict between predators and people in Ladakh. Cat News, 17, 18.
Abstract: During a six-week period in Hemis National Park, Ladakh, India, snow leopards killed 10 sheep and goats and one leopard gained access to a livestock pen and killed many of the animals inside. Dholes also killed sheep and goats, and a wolf killed a young horse. Residents routinely remove snow leopard cubs from their dens to limit future damage by this species. How to deal with the plight of the people living in the area while still protecting the endangered species are major concerns of the International Snow Leopard Trust, which manages Hemis National Park. lgh.
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Fox, J. L. (1986). Indo-US Snow Leopard Project, Progress Report on Field Work as of December 30, 1985 (Vol. 9). Seattle: Islt.
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Jackson, R., & Wangchuk, R. (2004). A Community-Based Approach to Mitigating Livestock Depredation by Snow Leopards (Vol. 9).
Abstract: Livestock depredation by the endangered snow leopard (Panthera uncia) _is an increasingly contentious issue in Himalayan villages, especially in or near protected areas. Mass attacks in which as many as 100 sheep and goats are killed in a single incident inevitably result in retaliation by local villagers. This article describes a community-based conservation initiative to address this problem in Hemis National Park, India. Human-wildlife conflict is alleviated by predator-proofing villagers' nighttime livestock pens and by enhancing household incomes in environmentally sensitive and culturally compatible ways. The authors have found that the highly participatory strategy described here (Appreciative Participatory Planning and Action-APPA) leads to a sense of project ownership by local stakeholders, communal empowerment, self-reliance, and willingness to co-exist with
snow leopards. The most significant conservation outcome of this process is the protection from retaliatory poaching of up to five snow leopards for every village's livestock pens that are made predator-proof._
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