Abstract: Background: The order Carnivora is well represented in India, with 58 of the 250 species found globally, occurring here. However, small carnivores figure very poorly in research and conservation policies in India. This is mainly due to
the dearth of tested and standardized techniques that are both cost effective and conducive to small carnivore studies in the field. In this paper we present a non-invasive genetic technique standardized for the study of Indian felids and canids with the use of PCR amplification and restriction enzyme digestion of scat collected in the field.
Findings: Using existing sequences of felids and canids from GenBank, we designed primers from the 16S rRNA region of the mitochondrial genome and tested these on ten species of felids and five canids. We selected restriction enzymes that would cut the selected region differentially for various species within each family. We produced a restriction digestion profile for the potential differentiation of species based on fragment patterns. To test our technique, we used felid PCR primers on scats collected from various habitats in India, representing varied environmental conditions. Amplification success with field collected scats was 52%, while 86% of the products used for restriction digestion could be accurately assigned to species. We verified this through sequencing. A comparison of costs across the various techniques currently used for scat assignment showed that this technique was the most practical and cost effective.
Conclusions: The species-specific key developed in this paper provides a means for detailed investigations in the future that focus on elusive carnivores in India and this approach provides a model for other studies in areas of Asia
where many small carnivores co-occur.

Abstract: Reports on conservation measures over the past 10 years. Notes current snow leopard exchange programs between zoos in the US and USSR and Europe. Describes status and reproductive success of the captive snow leopard population, list animals currently in captivity.

Abstract: It describes status of snow leopard in republics of the former Soviet Union, main reasons for poaching and negative and positive experience on species conservation. The total number of snow leopard in Kyrgyzstan and Tajikistan in the 1980's was at least 1200-1400. In Kazakhstan the snow leopard population totaled 180-200, in Uzbekistan around 100, and in Russia 150-200. The number and natural habitat of the species were in universal decline. Losses in Kyrgyzstan from poaching alone were no less than 30 animals per year in 1960-1980's. During this period the population in this area decreased by half, and the natural habitat by one third. With the break-up of the Soviet Union, poaching of the snow leopard and its pray grew by at least a factor of 3-4. It was estimated that 500-600 animals reduced the Kyrgyz snow leopard population in the 1990's. The population that remains today can barely be more than 150-200 individual snow leopards. The population has been physically decimated. Number of snow leopard in existence today in the former Soviet Union does not exceed 700-900 individual animals.