Abstract: The protection of Snow Leopards in the remote and economically disadvantaged Northern Areas of Pakistan needs local people equipped with the skills to gather and present information on the number and range of individual animals in their area. It is important for the success of a conservation campaign that the people living in the area are engaged in the conservation process. Snow Leopards are elusive and range through inhospitable terrain so direct study is difficult. Consequently the major goals for this project were twofold, to gather information on snow leopard distribution in this area and to train local university students and conservation management professionals in the techniques used for locating snow leopards without the need to capture or even see the animals. This project pioneered the use of DNA testing of field samples collected in Pakistan to determine the distribution of snow leopards and to attempt to identify individuals. These were collected in and around that country's most northerly national park, the Kunjurab National Park, which sits on the Pakistan China border. Though the Northern Areas is not a well developed part of Pakistan, it does possess a number of institutions that can work together to strengthen snow leopard conservation. The first of these is a newly established University with students ready to be trained in the skills needed. Secondly WWF-Pakistan has an office in the main town and a state of the art GIS laboratory in Lahore and already works closely with the Forest Department who manage the national park. All three institutions worked together in this project with WWF providing GIS expertise, the FD rangers, and the university students carrying out the laboratory work. In addition in the course of the project the University of the Punjab in Lahore also joined the effort, providing laboratory facilities for the students. As a result of this project maps have been produced showing the location of snow leopards in
two areas. Preliminary DNA evidence indicates that there is more than one animal in this
relatively small area, but the greatest achievement of this project is the training and
experience gained by the local students. For one student this has been life changing. Due to
the opportunities provided by this study the student, Nelofar gained significant scientific
training and as a consequence she is now working as a lecturer and research officer for the
Center for Integrated Mountain Research, New Campus University of the Punjab, Lahore
Pakistan

Abstract: The complete mitochondrial genome (mtDNA) of snow leopard Panthera uncia was obtained by using the polymerase chain reaction (PCR) technique based on the PCR fragments of 30 primers we designed. The entire mtDNA sequence was 16 773 base pairs (bp) in length, and the base composition was: A-5,357ª“,Ž+bp (31.9%); C-4,444ª”,Ž+bp (26.5%); G-2,428ª“,Ž+bp (14.5%); T-4,544ª”,Ž+bp (27.1%). The structural characteristics [0] of the P. uncia mitochondrial genome were highly similar to these of Felis catus, Acinonyx jubatus, Neofelis nebulosa and other mammals. However, we found several distinctive features of the mitochondrial genome of Panthera unica. First, the termination codon of COIII was TAA, which differed from those of F. catus, A. jubatus and N. nebulosa. Second, tRNASer (AGY), which lacked the ''DHU'' arm, could not be folded into the typical cloverleaf-shaped structure. Third, in the control region, a long repetitive sequence in RS-2 (32ª“,Ž+bp) region was found with 2 repeats while one short repetitive segment (9ª”,Ž+bp) was found with 15 repeats in the RS-3 region. We performed phylogenetic analysis based on a 3 816ª",Ž+bp concatenated sequence of 12S rRNA, 16S rRNA, ND2, ND4, ND5, Cyt b and ATP8 for P. uncia and other related species, the result indicated that P. uncia and P. leo were the sister species, which was different from the previous findings. (c) 2008 Springer Science+Business Media B.V.

Abstract: Workshop to develop a recovery plan for the wild north China tiger population. October 20th to 23th, 2000, Harbin.
Like the Siberian Tiger, the Far Eastern Leopard is one of China's largest Felidae and lives mainly in the eastern mountains of Jilin Province. The number of leopards is very low and it is even more endangered than the tiger. There is a very close relationship between leopard and tiger conservation, especially in areas where overlap occurs. In these areas, special emphasis has to be placed on each of the species' specific conservation needs. There is urgent need to step up our efforts to study and monitor leopard populations and to develop a conservation strategy. This document contains information of the status and main threats of the Far Eastern leopard and makes recommendations on needed conservation measures.

Abstract: Over a time of four years the bazars of Afghanistan were surveyed for furs of spotted wild cats, in particular leopards and snow leopards. In 2004 in Kabul a total of 28 furs of leopards were purchased by shopkeepers and 21 sold at an average price of 825 $. In the same year 25 furs of snow leopards were purchased and 19 sold to clients at an average price of 583 $. In 2006 at a single inspection double as many furs of leopards were found to be offered for sale in comparison to the whole year of 2004. Also prices had increased over the two years by 20 % to an average of 1037 $. Similarly the number of furs of snow leopards at 21 pieces was higher than in 2004, and the prices had increased to an average of 652 $. In 2007 investigations rendered more difficult, because the authorities had started to control the fur trade, and the results are not unequivocal. Clients were without any exception foreigners.
Surveys in 2004 in Mazar-e-Sharif, Kunduz, Takhar and Faiz Abad, in 2006 additionally in Baharak and Iskashem in the province of Badakhshan, revealed a regular trade in furs of spotted cats, however not as extensive as in Kabul. The most interesting finding was a fur of a cheetah in Mazar-e-Sharif, the first record of this species after 35 years.
From the surveys can be concluded that leopards still exist in the whole range of its distribution area in Afghanistan. However they don't allow any conclusion on the population size and its threat by hunting. In contrast to the leopard there exists a recent estimation of the population size of the snow leopard, saying that there are still 100 to 200 snow leopards living in Afghanistan. On the basis of these figures as well as the numbers of furs traded annually a Population and Habitat Viability Analysis was conducted. The result of this analysis is alarming. It has to be assumed that the snow leopard will be extinct in Afghanistan within the next ten years. To improve the protection of spotted cats in Afghanistan it needs both, a better implementation of the existing legislation as well as an awareness campaign among potential clients, i. e. foreigners living in Afghanistan.
The second part of this thesis deals with the question of subspecies of leopards in Afghanistan. Out of the 27 subspecies described four are believed to exist in Afghanistan. However, according to a molecularbiological revision of the species there occurs only one subspecies in Afghanistan, Panthera pardus saxicolor. To clarify the subspecies question various measures of furs had been taken in the bazars. The results revealed that the leopards in Afghanistan are the biggest of its species. However a further differentiation according to the area of origin within the country was not possible. Also the traditional differentiation on the basis of colours and patterns on the furs was not possible.
In contrast to the molecularbiological investigations published not only samples of zoo animals were available in this study but also samples from the wild. The own results confim that almost all leopards from Afghanistan and Iran belong to one and the same subspecies, P. p. saxicolor. Only in the most eastern part of Afghanistan, the Indian leopard, Panthera pardus fusca, can be found. The International Studbook for the Persian Leopard was analysed. The whole population derives from a few founder animals, which were imported in the midth fifties from Iran and in the late sixties from Afghanistan. To avoid inbreeding later on the Iranian and the Afghan lines were mixed. A female imported in 1968 from Kabul to Cologne is represented in each of the more than 100 today living animals.Mixing the two lines subsequently is justified by the genetic results of this study. Recently acquired animals from the Caucasus, however, should be tested genetically before integrating them into the zoo population.