Abstract: Based on the limited data available on snow leopard demography, we developed deterministic and stochastic stage-structured demographic models to study the population dynamics of this large cat. Our results reveal that even small leopard populations can persist provided their demographic parameters remain high, but less favorable scenarios would require larger population sizes. Population growth rate is more sensitive to breeder survivals than to any other parameters. A snow leopard population would start declining if yearly mortality claims more than 1/5 of the population. This study identifies poaching as a major threat to snow leopard survival and stresses the importance of long-term studies to better understand snow leopard population dynamics.

Abstract: Snow leopards (Panthera uncia) are elusive endangered carnivores found in remote mountain regions of Central Asia. New methods for identifying and counting snow leopards are needed for conservation and management efforts. To develop molecular genetic tools for individual identification of hair and faecal samples, we screened 50 microsatellite loci developed for the domestic cat (Felis catus) in 19 captive snow leopards. Forty-eight loci were polymorphic with numbers of alleles per locus ranging from two to 11. The probability of observing matching genotypes for unrelated individuals (2.1 x10-11) and siblings (7.5x10-5) using the 10 most polymorphic loci was low, suggesting that this panel would easily discriminate among individuals in the wild.

Abstract: Tremendous strides have been made in recent years to broaden our understanding of reproductive processes in nondomestic felid species and further our capacity to use this basic knowledge to control and manipulate reproduction of endangered cats. Much of that progress has culminated from detailed scientific studies conducted in nontraditional laboratory settings, frequently at collaborating zoological parks but also under more primitive conditions, including in the field. A mobile laboratory approach is described, which incorporates a diverse array of disciplines and research techniques. This approach has been extremely useful, especially for conducting gamete characterization and function studies as well as reproductive surveys, and for facilitating the development of assisted reproductive technology. With continuing advances in assisted reproduction in rare felids, more procedures are being conducted primarily as service-related activities, targeted to increase effectiveness of species propagation and population management. It can be a challenge for both investigators and institutional animal care and use committees (IACUCs) to differentiate these service-based procedures from traditional research studies (that require IACUC oversight). For research with rare cat species, multi-institutional collaboration frequently is necessary to gain access to scientifically meaningful numbers of study subjects. Similarly, for service-based efforts, the ability to perform reproductive procedures across institutions under nonstandard laboratory conditions is critical to applying reproductive sciences for managing and preserving threatened cat populations. Reproductive sciences can most effectively assist population management programs (e.g., Species Survival Plans) in addressing conservation priorities if these research and service- related procedures can be conducted “on the road” at distant national and international locales. This mobile laboratory approach has applications beyond endangered species research, notably for other scientific fields (e.g., studies of hereditary disease in domestic cat models) in which bringing the laboratory to the subject is of value.

Abstract: Bounded by two of the world's highest mountain ranges, the Great Himalaya and the Karakoram, Ladakh is a land of exhilarating mountain landscapes, rocky gorges and a unique cultural heritage. It is also home to distinctive wildlife such as the snow leopard, blue sheep and Tibetan wild ass, all living in a unique high altitude desert ecosystem. Not surprisingly, Ladakh is becoming a sought after tourist destination for international and domestic visitors alike. Over the past two decades tourism has grown substantially, although erratically, with both positive and less positive results for Ladakh's environment and people. People are recognizing that it is important to act now and engage in an informed dialogue in order to conserve the natural and cultural resources on which the future of tourism and related incomes depend. The Snow Leopard Conservancy (SLC) is working in collaboration with local communities and nongovernmental organizations to foster co-existence between people and predators like the endangered snow leopard by reducing livestock depredation losses and improving household incomes in environmentally friendly, socially responsible and economically viable ways. Well-balanced tourism is one income generating option.

Abstract: Predators have significant ecological impacts on the region's prey-predator dynamic and community structure through their numbers and prey selection. During April-December 2007, I conducted a research in Sagarmatha (Mt. Everest) National Park (SNP) to: i) explore population status and density of wild prey species; Himalayan tahr, musk deer and game birds, ii) investigate diet of the snow leopard and to estimate prey selection by snow leopard, iii) identify the pattern of livestock depredation by snow leopard, its mitigation, and raise awareness through outreach program, and identify the challenge and opportunities on conservation snow leopard and its co-existence with wild ungulates and the human using the areas of the SNP. Methodology of my research included vantage points and regular monitoring from trails for Himalayan tahr, fixed line transect with belt drive method for musk deer and game birds, and microscopic hair identification in snow leopard's scat to investigate diet of snow leopard and to estimate prey selection. Based on available evidence and witness accounts of snow leopard attack on livestock, the patterns of livestock depredation were assessed. I obtained 201 sighting of Himalayan tahr (1760 individuals) and estimated 293 populations in post-parturient period (April-June), 394 in birth period (July -October) and 195 November- December) in rutting period. In average, ratio of male to females was ranged from 0.34 to 0.79 and ratio of kid to female was 0.21-0.35, and yearling to kid was 0.21- 0.47. The encounter rate for musk deer was 1.06 and density was 17.28/km2. For Himalayan monal, the encounter rate was 2.14 and density was 35.66/km2. I obtained 12 sighting of snow cock comprising 69 individual in Gokyo. The ratio of male to female was 1.18 and young to female was 2.18. Twelve species (8 species of wild and 4 species of domestic livestock) were identified in the 120 snow leopard scats examined. In average, snow leopard predated most frequently on Himalayan tahr and it was detected in 26.5% relative frequency of occurrence while occurred in 36.66% of all scats, then it was followed by musk deer (19.87%), yak (12.65%), cow (12.04%), dog (10.24%), unidentified mammal (3.61%), woolly hare (3.01%), rat sp. (2.4%), unidentified bird sp. (1.8%), pika (1.2%), and shrew (0.6%) (Table 5.8 ). Wild species were present in 58.99% of scats whereas domestic livestock with dog were present in 40.95% of scats. Snow leopard predated most frequently on wildlife species in three seasons; spring (61.62%), autumn (61.11%) and winter (65.51%), and most frequently on domestic species including dog in summer season (54.54%). In term of relative biomass consumed, in average, Himalayan tahr was the most important prey species contributed 26.27% of the biomass consumed. This was followed by yak (22.13%), cow (21.06%), musk deer (11.32%), horse (10.53%), wooly hare (1.09%), rat (0.29%), pika (0.14%) and shrew (0.07%). In average, domestic livestock including dog were contributed more biomass in the diet of snow leopard comprising 60.8% of the biomass consumed whilst the wild life species comprising 39.19%. The annual prey consumption by a snow leopard (based on 2 kg/day) was estimated to be three Himalayan tahr, seven musk deer, five wooly hare, four rat sp., two pika, one shrew and four livestock. In the present study, the highest frequency of attack was found during April to June and lowest to July to November. The day of rainy and cloudy was the more vulnerable to livestock depredation. Snow leopard attacks occurred were the highest at near escape cover such as shrub land and cliff. Both predation pressure on tahr and that on livestock suggest that the development of effective conservation strategies for two threatened species (predator and prey) depends on resolving conflicts between people and predators. Recently, direct control of free – ranging livestock, good husbandry and compensation to shepherds may reduce snow leopard – human conflict. In long term solution, the reintroduction of blue sheep at the higher altitudes could also “buffer” predation on livestock.