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Author Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J.
Title Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples Type (up) Journal Article
Year 2014 Publication Conservation Genetic Resource Abbreviated Journal
Volume 6 Issue 2 Pages 369:373
Keywords Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification
Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic

information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many

noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the

domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard

populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and

population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers

facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.
Address
Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rakhee @ Serial 1428
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Author Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J.
Title Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples Type (up) Journal Article
Year 2014 Publication Conservation Genetic Resource Abbreviated Journal
Volume 6 Issue 2 Pages 369:373
Keywords Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification
Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic

information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many

noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the

domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard

populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and

population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers

facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.

endangered species
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Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rakhee @ Serial 1427
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Author Darehshuri, B.F.
Title Threatened cats of Asia Type (up) Journal Article
Year 1978 Publication Wildlife Abbreviated Journal
Volume 20 Issue 9 Pages 396-400
Keywords Asia, Acinonyx jubatus, Asiatic cheetah, cheetah, Siberian tiger, snow leopard
Abstract Man's hand is turned against the wild cats wherever they occur, often due to the value of their fur, but also because of the danger they sometimes pose to domestic stock and even human beings. All the larger Asian cats are threatened, and on this and the following pages we look at three of them – the Asiatic cheetah, the Siberian tiger, and the snow leopard.
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Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rana @ Serial 1116
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Author Chapron, G.
Title Re-wilding: other projects help carnivores stay wild Type (up) Journal Article
Year 2005 Publication Nature Abbreviated Journal
Volume 437 Issue Pages 318
Keywords Acinonyx jubatus, carnivore, coexistence, conservation, damage prevention, Panthera leo, snow leopard, survival, Uncia uncia
Abstract Letter to Nature Editor, in response to: In their plea for bringing Pleistocene wildlife to the New World (“Re-wilding North America” Nature 436, 913–914; 2005), Josh Donlan and colleagues do not discuss successful efforts to ensure long-term survival of large carnivores in Africa and Asia. A few examples are given.
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Corporate Author Thesis
Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rana @ Serial 1114
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Author Rothschild, B.M.; Rothschild, C.; Woods, R.J.
Title Inflammatory arthritis in large cats: An expanded spectrum of spondyloarthropathy Type (up) Journal Article
Year 1998 Publication Journal of Zoo and Wildlife Medicine Abbreviated Journal
Volume 29 Issue 3 Pages 279-284
Keywords Acinonyx-jubatus; cheetah; Felidae; Felis-concolor; mountain-lion; Felis-pardalis; ocelot; Panthera-leo; African-lion; Panthera-onca; jaguar; Panthera-pardus; leopard; Panthera-tigris; tiger; Panthera-uncia; snow-leopard; snow leopard; Acinonyx; jubatus; felis; concolor; mountain; lion; pardalis; panthera; leo; african; onca; pardus; tigris; uncia; browse; 1240
Abstract Spondyloarthropathy was documented for the first time in 14 (3.7%) of 386 large cats, affecting eight species belonging to three genera. The limited distribution of joint erosions, associated with spine and sacroiliac joint pathology, was indistinguishable from that occurring in humans with spondyloarthropathy of the reactive type. This form of inflammatory arthritis is almost twice as common as osteoarthritis (for felids as a whole), and animal well-being may be enhanced by its recognition and by initiation of specific treatment.
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Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Document Type: English Approved no
Call Number SLN @ rana @ 353 Serial 832
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Author Roth, T.L.; Howard, J.G.; Donoghue, A.M.; Swanson, W.F.; Wildt, D.E.
Title Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro Type (up) Journal Article
Year 1994 Publication J Reprod Fertil Abbreviated Journal
Volume 101 Issue 3 Pages 563-569
Keywords Animal; Carnivora; physiology; Cell; survival; Cells; Cultured; Comparative; study; Culture; Media; Female; Male; Pentoxifylline; Sperm; Motility; Ovum; Interactions; Spermatozoa; cytology; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 190
Abstract Electroejaculates from eight snow leopards were used to determine how the motility of spermatozoa was influenced by (i) type of media (Ham's F10, PBS, human tubal fluid or RPMI-1640); (ii) holding temperature (23 degrees C versus 37 degrees C); (iii) washing of spermatozoa and (iv) a sperm metabolic enhancer, pentoxifylline. The duration of sperm motility was assessed by evaluating samples in each treatment every hour for 6 h and a sperm motility index (a value combining percentage sperm motility and rate of forward progression) calculated. Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. During the 6 h co-incubation, the sperm motility index in PBS with pentoxifylline was greater (P < 0.05) than in PBS alone which, in turn, was greater (P < 0.05) than in the other three test media. Washing the spermatozoa enhanced (P < 0.05) motility in both PBS and PBS plus pentoxifylline relative to unwashed samples, but there was no effect (P > 0.05) of holding temperature. Pentoxifylline supplementation enhanced (P < 0.05) the proportion of cat eggs with bound, but not penetrated, snow leopard spermatozoa in the inner layer of the zona pellucida, and there were no spermatozoa in the perivitelline space.(ABSTRACT TRUNCATED AT 250 WORDS)
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Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0022-4251 ISBN Medium
Area Expedition Conference
Notes Document Type: eng Approved no
Call Number SLN @ rana @ 253 Serial 828
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Author Pollock, R.V.; Carmichael, L.E.
Title Use of modified live feline panleukopenia virus vaccine to immunize dogs against canine parvovirus Type (up) Journal Article
Year 1983 Publication Am J Vet Res Abbreviated Journal
Volume 44 Issue 2 Pages 169-175
Keywords Animal; Antibodies; Viral; biosynthesis; Dog; Diseases; microbiology; prevention; control; Dogs; Dose; Response; Relationship; Immunologic; Parvoviridae; immunology; Parvovirus; Feline; growth; development; Support; Non-U.S.Gov't; Vaccines; Attenuated; Virus; veterinary; Replication; browse; 350
Abstract Modified live feline panleukopenia virus (FPLV) vaccine protected dogs against canine parvovirus (CPV) infection. However, unlike the long- lived (greater than or equal to 20-month) immunity engendered by CPV infection, the response of dogs to living FPLV was variable. Doses of FPLV (snow leopard strain) in excess of 10(5.7) TCID50 were necessary for uniform immunization; smaller inocula resulted in decreased success rates. The duration of immunity, as measured by the persistence of hemagglutination-inhibiting antibody, was related to the magnitude of the initial response to vaccination; dogs with vigorous initial responses resisted oronasal CPV challenge exposure 6 months after vaccination, and hemagglutination-inhibiting antibodies persisted in such dogs for greater than 1 year. Limited replication of FPLV in dogs was demonstrated, but unlike CPV, the feline virus did not spread to contact dogs or cats. Adverse reactions were not associated with living FPLV vaccination, and FPLV did not interfere with simultaneous response to attenuated canine distemper virus.
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Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0002-9645 ISBN Medium
Area Expedition Conference
Notes Document Type: eng Approved no
Call Number SLN @ rana @ 69 Serial 784
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Author Macdonald, A.A.; Johnstone, M.
Title Comparative anatomy of the cardiac foramen ovale in cats (Felidae), dogs (Canidae), bears (Ursidae) and hyaenas (Hyaenidae) Type (up) Journal Article
Year 1995 Publication J Anat Abbreviated Journal
Volume 186 ( Pt 2) Issue Pages 235-243
Keywords Animal; Newborn; Bears: anatomy & histology; Carnivora: anatomy & histology: embryology; Cats: anatomy & histology; Dogs: anatomy & histology; Heart Septum: anatomy & histology: embryology; Support,Non-U.S.Gov't; bear; anatomy; histology; Carnivora; embryology; Cats; cat; bears; Dog; Dogs; heart; septum; browse; non; gov't; us; government; 160
Abstract The structure of the foramen ovale from 16 species representing 4 carnivore families, the Felidae, Canidae, Ursidae and Hyaenidae, was studied using the scanning electron microscope. The Felidae were represented by 9 domestic cat fetuses (Felis catus), 2 snow leopard neonates (Uncia uncia), an ocelot neonate (Leopardus pardalis), 2 lion neonates (Panthera leo), a panther neonate (Panthera pardus) and 3 tigers (Neofelis tigris), comprising 2 fetuses and a neonate. The Canidae were represented by a golden jackal neonate (Canis aureus), a newborn wolf (Canis lupus), 8 domestic dog fetuses (Canis familiaris), 3 red fox neonates (Vulpes vulpes) and a dhole neonate (Cuon alpinus). The Ursidae were represented by a brown bear neonate (Ursus arctos), a day-old grizzly bear cub (Ursus arctos horribilis), a polar bear neonate (Ursus maritimus), and 2 additional bear fetuses (species unknown). The Hyaenidae were represented by a striped hyaena neonate (Hyaena hyaena). In each species, the foramen ovale, when viewed from the terminal part of the caudal vena cava, had the appearance of a short tunnel. A thin fold of tissue, the developed remains of the embryonic septum primum, extended from the distal end of the caudal vena cava for a variable distance into the lumen of the left atrium and contributed towards the 'tunnel' appearance in all specimens. It constituted a large proportion of the tube, and its distal end was straight-edged. There was fibrous material underlying the endothelium of the flap, the apparent morphology of which suggested that it comprised cardiac muscle.(ABSTRACT TRUNCATED AT 250 WORDS)
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Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0021-8782 ISBN Medium
Area Expedition Conference
Notes Document Type: eng Approved no
Call Number SLN @ rana @ 269 Serial 632
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Author Johnston, L.A.; Donoghue, A.M.; O'Brien, S.J.; Wildt, D.E.
Title Rescue and maturation in vitro of follicular oocytes collected from nondomestic felid species Type (up) Journal Article
Year 1991 Publication Biol Reprod Abbreviated Journal
Volume 45 Issue 6 Pages 898-906
Keywords Animal; Carnivora; anatomy; histology; physiology; Cells; Cultured; Female; Fertilization; Vitro; inVitro; Male; Oocytes; cytology; growth; development; ovarian; Follicle; Spermatozoa; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 230
Abstract The potential for rescuing immature oocytes from the ovaries of females of rare felid species which die or undergo medical ovariohysterectomy was evaluated. Ovaries were recovered from 13 species representing 35 individuals in good-to-poor health. Although the majority of females were 10 yr of age or older and in fair-to-poor health, a total of 846 oocytes were recovered of which 608 (71.9%) were classified as fair-to- excellent quality. One hundred of these oocytes were used for initial maturation classification and as parthogenetic controls. Overall, of the 508 fair-to-excellent quality oocytes placed in culture, 164 (32.3%) matured to metaphase II in vitro. For species in which 3 or more individuals yielded oocytes, mean oocyte maturation rates were as follows: 36.2%, tiger; 27.9% leopard; and 8.3%, cheetah. In vitro insemination of oocytes resulted in fertilization (2 polar bodies, 2 pronuclei, or cleavage) rates of 9.1% to 28.6% (leopard) using homologous fresh spermatozoa and 4.0% (lion) to 40.0% (puma) using homologous frozen-thawed spermatozoa. Inseminations using heterologous (domestic cat) spermatozoa also resulted in fertilized oocytes in the tiger, leopard, snow leopard, puma, serval, and Geoffroy's cat (range in fertilization rate, 5.0% for leopard to 46.2% for puma). Cleaved embryos resulted from the insemination of leopard oocytes with homologous sperm (n = 1 embryo) and puma oocytes with domestic cat sperm (n = 3 embryos). These results demonstrate that immature ovarian oocytes from rare felid species can be stimulated to mature in vitro despite an excision-to-culture interval as long as 36 h.(ABSTRACT TRUNCATED AT 250 WORDS)
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Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0006-3363 ISBN Medium
Area Expedition Conference
Notes Document Type: eng Approved no
Call Number SLN @ rana @ 172 Serial 503
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Author Gosselin, S.J.; Loudy, D.L.; Tarr, M.J.; Balistreri, W.F.; Setchell, K.D.; Johnston, J.O.; Kramer, L.W.; Dresser, B.L.
Title Veno-occlusive disease of the liver in captive cheetah Type (up) Journal Article
Year 1988 Publication Vet Pathol Abbreviated Journal
Volume 25 Issue 1 Pages 48-57
Keywords Animal; Feed; analysis; Carnivora; Cheetahs; Female; Hepatic; Veno; Occlusive; Disease; pathology; veterinary; Histocytochemistry; Liver; ultrastructure; Male; Microscopy; Electron; Support; Non-U.S.Gov't; Vitamin A; browse; non; us; gov't; government; 280
Abstract Liver tissues from 126 captive cheetah were evaluated by light microscopy and histochemistry; eight animals were evaluated by electron microscopy. The main hepatic lesion, a vascular lesion resembling veno- occlusive disease (VOD) of the liver and characterized by subendothelial fibrosis and proliferation of smooth muscle-like cells in the central veins, was seen in 60% of the sexually mature cheetah. Although this hepatic vascular lesion was seen in cheetah as young as 1 year of age, the most severe lesions, usually associated with liver failure, were found in cheetah between the ages of 6 and 11. There was no sex predisposition, and in approximately 40% of the VOD cases, liver disease was not suspected clinically or at necropsy. VOD was found in other felidae, especially in the snow leopard. High levels of vitamin A in livers, as well as in diets of the cheetah, could be a contributing factor in the development of VOD in some groups of cheetah.
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Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0300-9858 ISBN Medium
Area Expedition Conference
Notes Document Type: Eng Approved no
Call Number SLN @ rana @ 127 Serial 339
Permanent link to this record