Abstract: Conservation strategies for apex predators, like the snow leopard (Panthera uncia), depend on a robust understanding of their dietary preferences, prey abundance, and adaptability to changing ecological conditions. To address these critical conservation concerns, this study presents a comprehensive evidence on prey availability and preferences for snow leopards in the Lapchi Valley in the Nepal Himalayas from November 2021 to March 2023. Field data were collected through the installation of twenty-six camera traps at 16 strategically chosen locations, resulting in the recording of 1228 events of 19 mammalian species, including domesticated livestock. Simultaneously, the collection of twenty snow leopard scat samples over 3800 m above sea level allowed for a detailed dietary analysis. Photo capture rate index and biomass composition analysis were carried out and seasonal prey availability and consumption were statistically analyzed. A total of 16 potential prey species for the snow leopard were documented during the study period. Himalayan musk deer (Moschus leucogaster) was the most abundant prey species, but infrequent in the diet suggesting that are not the best bet prey for the snow leopards. Snow leopards were found to exhibit a diverse diet, consuming eleven prey species, with blue sheep (Pseudois nayaur) being their most consumed wild prey and horses as their preferred livestock. The Pianka’s index of dietary niche overlap between the summer and winter seasons were 0.576, suggesting a pronounced seasonal variation in food preference corroborating with the prey availability. The scarcity of larger preys in winter is compensated by small and meso-mammals in the diet, highlighting the snow leopard’s capacity for dietary plasticity in response to the variation in resource availability. This research suggests for the utilization of genetic tools to further explore snow leopard diet composition. Additionally, understanding transboundary movements and conducting population assessments will be imperative for the formulation of effective conservation strategies.

Abstract: Blue sheep (Pseudois nayaur) are the principal prey of the endangered snow leopard (Panthera uncia) in the Himalayas and adjacent ranges. We studied group size and population composition of blue sheep in Manang District, Annapurna Conservation Area, Nepal. Overall mean group size was 15.6 (SE = 1.3), but it varied seasonally (P lt 0.001), with significantly smaller groups in winter than in other seasons. Mixed groups were most numerous in all seasons, and there was no evidence of sexual segregation. Yearling sex ratio (93.7 M:100 F) did not vary seasonally, nor did the ratio deviate from parity. Adult sex ratio showed a seasonal pattern favoring males post-parturition but female-biased during the rut and pre-parturition. Seasonal variation in sex-specific mortality is offered as a plausible explanation for the observed pattern in adult sex ratio.

Abstract: The annual reproductive cycle of the male snow leopard (Panthera uncia) was characterized by evaluating seminal and endocrine traits monthly. Testicular volume was greatest (P < 0.05) during the winter months when the quality of ejaculate was optimal. Ejaculate volume, total sperm concentration ml-1, motile sperm concentration per ejaculate, sperm morphology and sperm motility index were lowest during the summer and autumn months compared with the winter and spring. Peripheral LH, FSH and testosterone concentrations were also lowest during the summer months, increasing during the autumn just before the increase in semen quality, and were maximal during the winter months. There was a direct relationship (P < 0.01) between: (1) testosterone and testicular volume, total sperm concentration ml-1, motile sperm concentration per ejaculate and ejaculate volume, and (2) LH and testicular volume and motile sperm concentration per ejaculate. In summary, although spermatozoa were recovered throughout the year, optimal gamete quality was observed during the winter and spring. Although previous studies in felids have demonstrated seasonal effects on either seminal or endocrine traits, this is the first study to demonstrate a distinct effect of season on both pituitary and testicular function.

Abstract: Conservation practices are supposed to get refined by advancing scientific knowledge. We study this phenomenon in the context of monitoring tiger populations in India, by evaluating the ‘pugmark census method’ employed by wildlife managers for three decades. We use an analytical framework of modern animal population sampling to test the efficacy of the pugmark censuses using scientific data on tigers and our field observations. We identify three critical goals for monitoring tiger populations, in order of increasing sophistication: (1) distribution mapping, (2) tracking relative abundance, (3) estimation of absolute abundance. We demonstrate that the present census-based paradigm does not work because it ignores the first two simpler goals, and targets, but fails to achieve, the most difficult third goal. We point out the utility and ready availability of alternative monitoring paradigms that deal with the central problems of spatial sampling and observability. We propose an alternative sampling-based approach that can be tailored to meet practical needs of tiger monitoring at different levels of refinement.