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International Snow Leopard Trust. (1996). Snow Line (Vol. XIV).
Abstract: Newsletter of International Snow Leopard Trust
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Roth, T. L., Swanson, W. F., Wildt, D. E., Collins, D., Burton, M., & Garell, D. M. (1996). Snow leopard (Panthera uncia) spermatozoa are sensitive to alkaline pH, but motility in vitro is not influenced by protein or energy supplements (Vol. 17).
Abstract: To better understand the biology of snow leopard spermatozoa and to facilitate developing assisted reproduction, a series of studies was conducted to: 1) identify the component(s) of complex culture media responsible for the detrimental effect on sperm survival in vitro, 2) optimize medium for supporting sperm viability, and 3) evaluate sperm capacitation in vitro. Constituents of complex media were added systematically to phosphate-buffered saline (PBS) to isolate the factor(s) influencing snow leopard sperm motility in vitro. Sperm capacitation was also assessed following incubation in PBS with bovine serum albumin (BSA), fetal calf serum (FCS), or heparin. For maintaining sperm motility, there was no benefit (P ? 0.05) to supplementing PBS with low (5%) or high (20%) concentrations of snow leopard serum (SLS) versus FCS or BSA. Likewise, adding supplemental energy substrates (pyruvate, glucose, lactate, or glutamine) did not enhance or hinder (P ? 0.05) sperm motility. However, motility rapidly decreased (P < 0.05) with the addition of NaHCO3 to PBS or Ham's F10 nutrient mixture. Surprisingly, Ham's F10 with no buffering component or with both NaHCO3 and N-Z-hydroxyethylpiperazine-N'-2- ethanesulfonic acid (HEPES) maintained sperm motility at levels similar (P ? 0.05) to PBS. Although sperm motility in all treatments decreased with time, there was a strong inverse relationship (P < 0.01; r = 0.90) between motility and sample pH at 6 hours. Spermatozoa incubated in PBS containing FCS, BSA, or heparin did not undergo the acrosome reaction when exposed to calcium ionophore. In summary, alkaline pH has a profound detrimental effect on snow leopard sperm motility, and capacitation does not occur under conditions that normally promote this event in other felid species. These results clearly demonstrate a high degree of interspecific variation among felids in fundamental sperm function, and they provide evidence for the necessity of basic research when developing assisted reproduction in little-studied nondomestic species.
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Grachev Yu.A. (1996). Snow leopard (Vol. Vol.1.Animals. Part 1.Vertebrates.).
Abstract: Status: rare (Category III). Distribution: Tien Shan mountains, Tarbagatai, Saur and Altai mountains. Total number in Kazakhstan does not exceed 200 individuals. The main threats are poaching and reduction number of preys. In Almaty Zoo captive breeding was successful in 1976 and 1985. Snow leopard is protected in Aksu Jabagly, Almaty and Markakol nature reserves. To ensure the survival of the species it is necessary to establish a reserve in Dzhungar mountains and to improve protection in existing nature reserves.
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Oli, M. K. (1996). Seasonal patterns in habitat use of blue sheep Pseudois nayaur (Artiodactyla, Bovidae) in Nepal. Mammalia, 60(2), 187–193.
Abstract: Blue sheep (Pseudois nayaur) are the main prey of the endangered snow leopard (Panthera uncia) as well as an important game species in Nepal. A knowledge of how blue sheep utilize their habitat is essential for the scientific management of the sheep and for the conservation of the snow leopard, but we only have a limited understanding of this aspect of blue sheep ecology. I studied the habitat use pattern of blue sheep by direct observation in the Anna-purna Conservation Area, Nepal where they occur sympatrically with the snow leopard. The sheep used grassland habitats more frequently during pre-parturition (spring) and post-parturition (autumn) than other habitat types, but scrub and grassland habitats were used equally frequently during the rut (winter). The sheep used smooth undulating slopes of medium steepness (<40 degrees) on southerly aspects within the elevation range of 4,200-4,600 m most frequently in all seasons, and there was no evidence of seasonal migration along the elevation gradient. When not in broken landforms (e.g., cliff, landslides), the sheep maintained proximity (less than or equal to 150 m) to such features suggesting their importance as escape cover (i.e., from predators). The use of habitat components by blue sheep appeared to be related to the distribution of foraging areas and escape cover.
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Oli, M. K., & Rogers, E. M. (1996). Seasonal pattern in group size and population composition of blue sheep in Manang, Nepal. Journal of Wildlife Management, 60(4), 797–801.
Abstract: Blue sheep (Pseudois nayaur) are the principal prey of the endangered snow leopard (Panthera uncia) in the Himalayas and adjacent ranges. We studied group size and population composition of blue sheep in Manang District, Annapurna Conservation Area, Nepal. Overall mean group size was 15.6 (SE = 1.3), but it varied seasonally (P lt 0.001), with significantly smaller groups in winter than in other seasons. Mixed groups were most numerous in all seasons, and there was no evidence of sexual segregation. Yearling sex ratio (93.7 M:100 F) did not vary seasonally, nor did the ratio deviate from parity. Adult sex ratio showed a seasonal pattern favoring males post-parturition but female-biased during the rut and pre-parturition. Seasonal variation in sex-specific mortality is offered as a plausible explanation for the observed pattern in adult sex ratio.
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Polking, V. F. (1996). Schneelleopard. Das Tier, , 8–15.
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Johnson, W. E., Dratch, P. A., Martenson, J. S., & O'Brien, S. J. (1996). Resolution of recent radiations within three evolutionary lineages of Felidae using mitochondrial restriction fragment length polymorphism variation. Journal of Mammalian Evolution, 3(2), 97–120.
Abstract: Patterns of mitochondrial restriction fragment length polymorphism (RFLP) variation were used to resolve more recent relationships among the species of the Felidae ocelot lineage, domestic cat lineage, and pantherine lineage. Twenty-five of 28 restriction enzymes revealed site variation in at least 1 of 21 cat species. The ocelot lineage was resolved into three separate sister taxa groups: Geoffroy's cat (Oncifelis geoffroyi) and kodkod (O. guigna), ocelot (Leopardus pardalis) and margay (L. wiedii), and pampas cat (Lynchailurus colocolo) and most of the tigrina samples (Leopardus tigrina). Within the domestic cat lineage, domestic cat (Felis catus), European wild cat (F. silvestris), and African wild cat (F. libyca) formed a monophyletic trichotomy, which was joined with sand cat (F. margarita) to a common ancestor. Jungle cat (F. chaus) and black-footed cat (F. nigripes) mtDNAs diverged earlier than those of the other domestic cat lineage species and are less closely related. Within the pantherine lineage, phylogenetic analysis identified two distinct groups, uniting lion (P. leo) with leopard (P. pardus) and tiger (P. tigris) with snow leopard (P. uncia).
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Jackson, R. M., Ahlborn, G., Gurung, M., & Ale, S. (1996). Reducing livestock depredation losses in the Nepalese Himalaya. Proc.Vertebr.Pest Conf, 17, 241–247.
Abstract: The authors investigated livestock depredation patterns of snow leopards on the northern slopes of the Himalayans near the villages of Manang and Khangshar, Nepal. Information is discussed on the relationships among livestock loss, endangered species management, public relations/conservation issues, and cooperative efforts among institutions involved in the decision making process. A plan is devised for alleviating livestock loss and protecting endangered species in the area. pcp
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Anonymous. (1996). Preserving the snow leopard and its habitat. The Rolex Awards for Enterprise Journal, 3.
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Saberwal, V. K. (1996). Pastoral Politics:gaddi grazing, degradation and biodiversity conservation in Himachal Pradesh, India. Conservation Biology, 10, 741–749.
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