Abstract: Liver tissues from 126 captive cheetah were evaluated by light microscopy and histochemistry; eight animals were evaluated by electron microscopy. The main hepatic lesion, a vascular lesion resembling veno- occlusive disease (VOD) of the liver and characterized by subendothelial fibrosis and proliferation of smooth muscle-like cells in the central veins, was seen in 60% of the sexually mature cheetah. Although this hepatic vascular lesion was seen in cheetah as young as 1 year of age, the most severe lesions, usually associated with liver failure, were found in cheetah between the ages of 6 and 11. There was no sex predisposition, and in approximately 40% of the VOD cases, liver disease was not suspected clinically or at necropsy. VOD was found in other felidae, especially in the snow leopard. High levels of vitamin A in livers, as well as in diets of the cheetah, could be a contributing factor in the development of VOD in some groups of cheetah.

Abstract: Snow leopard conservation work in Bhutan dates back to 1999 and 2000 when the International Snow Leopard Trust-in collaboration with the Royal Government of Bhutan and World Wildlife Fund-initiated a training workshop. More than 30 government staff were trained in SLIMS survey techniques. As a part of the training exercise, a preliminary survey on snow leopard was also carried out using the SLIMS methods in Jigme Dorji Wangchuck National Park. Based on the survey results, we estimated there was a population of 100 snow leopards in the wild and 10,000 km2 of habitat. In 2005, World Wildlife Fund (WWF) organized the WWF/South Asia Regional Workshop on Snow leopard Conservation in Bhutan. Both regional (Bhutan, India, China, Nepal and Pakistan) and international experts revisited the snow leopard programs and developed a work plan for the overall conservation of the snow leopard in the region. This led to WWF's Regional Snow leopard Conservation Strategy. WWF is pleased to submit our final report to the International Snow Leopard Trust on the oneyear, $8,000 grant in support of Snow Leopard and Prey Population Conservation in Bhutan. With the support of the Snow Leopard Trust, we have made great strides towards achieving our goal for this project: To determine the current status of snow leopard and ungulate prey populations in prime snow leopard habitats. Major accomplishments and activities completed thanks to the generous support of the International Snow Leopard Trust include:
 Signed of a Terms of Reference between Royal Government, International Snow Leopard
Trust – India, World Wildlife Fund and International Snow Leopard Trust -US;
 Developed a joint revised project work plan; and
 Purchased basic field supplies and equipment needed for the surveys planned.

Abstract: Ovaries of the seasonally-breeding snow leopard (Uncia uncia) were examined to determine whether they were responsive to exogenous gonadotrophins throughout the year. The potential of laparoscopic artificial insemination (AI) also was assessed for producing offspring. During the non-breeding, pre-breeding, breeding and post-breeding seasons, females (n = 20) were treated with a standardized, dual- hormone regimen given intramuscularly (600 I.U. of equine chorionic gonadotrophin followed 80-84 h later with 300 I.U. of human chorionic gonadotrophin (hCG)). Laparoscopy was performed 45-50 h after administration of hCG, and all ovarian structures were described. Females with fresh corpora lutea (CL) were inseminated, and anovulatory females were subjected to follicular aspiration to examine oocyte quality. Snow leopards responded to exogenous gonadotrophins throughout the year. Mean number of total ovarian structures (distinct follicles mature in appearance plus CL) did not differ (P > or = 0.05) with season, but the proportion of CL: total ovarian structures was greater (P < 0.01) for the breeding season compared with all other seasons. The proportion of females ovulating was greater (P < 0.05) during the breeding and post-breeding seasons than during the pre-breeding and non- breeding seasons respectively. No Grade-1 quality oocytes were recovered from follicles of anovulatory females. Serum concentrations of oestradiol-17 beta appeared elevated in all females, and neither oestradiol-17 beta concentrations nor progesterone concentrations differed (P > or = 0.05) among seasons. Of 15 females artificially inseminated, the only one that was inseminated in the non-breeding season became pregnant and delivered a single cub. This is the first successful pregnancy resulting from AI in this endangered species.