Abstract: The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards.

Abstract: From September to November 2004 we conducted surveys of snow leopard Uncia uncia signs in three major valleys in Sagarmatha (Mount Everest) National Park in Nepal using the Snow Leopard Information Management System, a standardized survey technique for snow leopard research. We walked 24 transects covering c. 14 km and located 33 sites with 56 snow leopard signs, and 17 signs incidentally in other areas. Snow leopards appear to have re-inhabited the Park, following their disappearance c. 40 years ago, apparently following the recovery of Himalayan tahr Hemitragus jemlahicus and musk deer Moschus chrysogaster populations. Taken together the locations of all 73 recent snow leopard signs indicate that the species is using predominantly grazing land and shrubland/ open forest at elevations of 3,000-5,000 m, habitat types that are also used by domestic and wild ungulates. Sagarmatha is the homeland of c. 3,500 Buddhist Sherpas with .3,000 livestock. Along with tourism and associated developments in Sagarmatha, traditional land use practices could be used to ensure coexistence of livestock and wildlife, including the recovering snow leopards, and ensure the wellbeing of the Sherpas.

Abstract: The Snow Leopard (Uncia uncia) investigation in the Tomur area is the second step of the “Project of Snow Leopard Study in Xinjiang”. In this part of the project, we collected information on the distribution , abundance and population size of the snow leopard in this area. The investigation lasted for 3 weeks, between October 17 and November 7th, 2004. During the 22 days of field work, we surveyed 4 different places in Wensu County, Aksu District: e.g. Pochenzi and the Muzat River area, Bozdun and the Little Kuzbay River area, Yinyar and the Tomur River area, Taglak and the Qiong Tailan River area. The 4 main areas, along with a few other valleys, covered most of the Tomur National Conservation Zone. In total, we ran 42 transects. In 15 transects, we found signs left by snow leopards. We also collected 15 fecal samples for diet analysis. This time we interviewed nearly 90 local people from different nationalities: e.g. Han (Chinese), Uygur and Kyrgyz people, including herdsmen, geologists, mineworkers, drivers, veterinarians, businessmen, forest officials, soldiers and policemen. They provided us with an array of information on the historical and current distribution and abundance of the snow leopard in this area.