| Records |
| Author |
Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. |
Title  |
Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces |
Type |
Journal Article |
| Year |
1994 |
Publication |
Biol Reprod |
Abbreviated Journal |
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| Volume |
51 |
Issue |
4 |
Pages |
776-786 |
| Keywords |
Animal; Carbon; Radioisotopes; Carnivora; Cats; Chromatography; High; Pressure; Liquid; Comparative Study; Estradiol; metabolism; Estrone; feces; chemistry; Female; Ovary; physiology; Pregnancy; Progesterone; Pseudopregnancy; Support; Non-U.S.Gov't; browse; non; government; gov't; us; 170 |
| Abstract |
Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species. |
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0006-3363 |
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| Notes |
Document Type: eng |
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no |
| Call Number |
SLN @ rana @ 251 |
Serial |
198 |
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| Author |
Dhungel, S. |
| Title |
Conservation of the Snow Leopard in Nepal |
Type |
Conference Article |
| Year |
1994 |
Publication |
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Abbreviated Journal |
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| Volume |
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Issue |
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Pages |
47-50 |
| Keywords |
Nepal; conservation; livestock; herder; herders; poaching; hunting; pelts; fur; coat; skin; distribution; status; behavior; predator; prey; breeding; Himalaya; park; parks; reserve; refuge; protected-area; biology; habitat; scent; spray; tracks; scrapes; home-range; mating; bharal; blue-sheep; goral; tahr; musk-deer; blue; sheep; browse; musk; deer; 3030 |
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Islt |
Place of Publication |
Usa |
Editor |
Fox, J.L.; Jezing, D. |
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| Notes |
full text available at URLTitle, Monographic: Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 |
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no |
| Call Number |
SLN @ rana @ 306 |
Serial |
241 |
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| Author |
Harris, R.B. |
| Title |
Dealing with uncertainty in counts of mountain ungulates |
Type |
Conference Article |
| Year |
1994 |
Publication |
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Abbreviated Journal |
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Issue |
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Pages |
105-111 |
| Keywords |
ungulates; blue-sheep; argali; tahr; ibex; prey; predator; status; population; asia; blue; sheep; browse; 3260 |
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Islt |
Place of Publication |
Usa |
Editor |
J.L.Fox; Jizeng, D. |
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Full Text at URLTitle, Monographic: Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 |
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no |
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SLN @ rana @ 224 |
Serial |
372 |
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| Author |
Xinchun, M. |
| Title |
Distribution in the wild and the captive raising of snow leopards in Xinjiang, China |
Type |
Conference Article |
| Year |
1994 |
Publication |
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Abbreviated Journal |
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Issue |
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Pages |
157-162 |
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distribution; habitat; ibex; blue-sheep; argali; hides; skins; pelts; furs; coats; zoos; zoo; China; captivity; care; husbandry; feeding; diet; housing; Disease; blue; sheep; browse; 3770 |
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Islt |
Place of Publication |
Usa |
Editor |
J.L.Fox; D.Jizeng |
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Full text at URLTitle, Monographic: Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 |
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no |
| Call Number |
SLN @ rana @ 267 |
Serial |
1031 |
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| Author |
Rawat, G.S.; Pandey, S.; Panwar, H.S. |
| Title |
Eco-development and management needs for snow leopard conservation in Himalayan Protected Areas |
Type |
Conference Article |
| Year |
1994 |
Publication |
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Abbreviated Journal |
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| Volume |
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Issue |
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Pages |
297-303 |
| Keywords |
development; eco-development; management; conservation; protected-areas; parks; reserves; refuge; park; preserve; Himalaya; Pin-valley; Himachal; herders; livestock; fire-wood; browse; eco; protected; areas; firewood; fire; 3810 |
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Islt |
Place of Publication |
Usa |
Editor |
J.L.Fox; D.Jizeng |
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Full Text at URLTitle, Monographic: Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 |
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no |
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SLN @ rana @ 249 |
Serial |
809 |
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| Author |
Koshkarev, E. |
| Title |
Evaluation of the presence of snow leopard and ibex in Southern Siberia |
Type |
Conference Article |
| Year |
1994 |
Publication |
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Abbreviated Journal |
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Issue |
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Pages |
17-27 |
| Keywords |
status; distribution; siberia; predator; prey; Russia; Soviet-Union; Ussr; ibex; Sayan; Altai; Altay; Kazakhstan; fossil; ice-age; protected-areas; parks; preserves; reserves; refuge; kazakstan; browse; soviet union; soviet; union; ice age; protected area; protected areas; protected; area; areas; 2860 |
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Islt |
Place of Publication |
Seattle/USA |
Editor |
J.Fox; D.Jizeng |
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full text at URLTitle, Monographic: Proceedings of Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 |
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no |
| Call Number |
SLN @ rana @ 222 |
Serial |
561 |
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Roth, T.L.; Howard, J.G.; Donoghue, A.M.; Swanson, W.F.; Wildt, D.E. |
| Title |
Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro |
Type |
Journal Article |
| Year |
1994 |
Publication |
J Reprod Fertil |
Abbreviated Journal |
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| Volume |
101 |
Issue |
3 |
Pages |
563-569 |
| Keywords |
Animal; Carnivora; physiology; Cell; survival; Cells; Cultured; Comparative; study; Culture; Media; Female; Male; Pentoxifylline; Sperm; Motility; Ovum; Interactions; Spermatozoa; cytology; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 190 |
| Abstract |
Electroejaculates from eight snow leopards were used to determine how the motility of spermatozoa was influenced by (i) type of media (Ham's F10, PBS, human tubal fluid or RPMI-1640); (ii) holding temperature (23 degrees C versus 37 degrees C); (iii) washing of spermatozoa and (iv) a sperm metabolic enhancer, pentoxifylline. The duration of sperm motility was assessed by evaluating samples in each treatment every hour for 6 h and a sperm motility index (a value combining percentage sperm motility and rate of forward progression) calculated. Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. During the 6 h co-incubation, the sperm motility index in PBS with pentoxifylline was greater (P < 0.05) than in PBS alone which, in turn, was greater (P < 0.05) than in the other three test media. Washing the spermatozoa enhanced (P < 0.05) motility in both PBS and PBS plus pentoxifylline relative to unwashed samples, but there was no effect (P > 0.05) of holding temperature. Pentoxifylline supplementation enhanced (P < 0.05) the proportion of cat eggs with bound, but not penetrated, snow leopard spermatozoa in the inner layer of the zona pellucida, and there were no spermatozoa in the perivitelline space.(ABSTRACT TRUNCATED AT 250 WORDS) |
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0022-4251 |
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| Notes |
Document Type: eng |
Approved |
no |
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SLN @ rana @ 253 |
Serial |
828 |
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| Author |
Qiseng, Y. |
| Title |
Further Study on the Geographical Distribution and Conservation of Snow Leopard in Qinghai, China |
Type |
Conference Article |
| Year |
1994 |
Publication |
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Abbreviated Journal |
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Issue |
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Pages |
73-78 |
| Keywords |
China; Qinghai; distribution; status; surveys; interviews; poaching; hunters; hunting; herders; collection; conservation; browse; 3750 |
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Islt |
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Usa |
Editor |
J.L.Fox; D.Jizeng |
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Full Text at URLTitle, Monographic: Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 |
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no |
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SLN @ rana @ 247 |
Serial |
801 |
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| Author |
Oli, M. |
| Title |
Ghost in the snow |
Type |
Magazine Article |
| Year |
1994 |
Publication |
BBC Wildlife |
Abbreviated Journal |
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Issue |
August |
Pages |
30-35 |
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BBC |
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English |
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1287 |
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| Author |
Xuedong, X.; Jackson, R.; Zongyi, W. |
| Title |
Herd characteristics and habitat use of a blue sheep population in the Qomolangma Nature Preserve |
Type |
Conference Article |
| Year |
1994 |
Publication |
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Abbreviated Journal |
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Issue |
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Pages |
97-104 |
| Keywords |
Nepal; prey; Qomolangma; protected-area; parks; reserves; refuge; blue-sheep; habitat; Pseudois-nayaur; blue; sheep; browse; protected; area; protected area; 3480 |
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Islt |
Place of Publication |
Usa |
Editor |
J.L.Fox; D.Jizeng |
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| Notes |
Full text at URLTitle, Monographic: Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 |
Approved |
no |
| Call Number |
SLN @ rana @ 263 |
Serial |
1041 |
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