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Author De Groot, H.; Van Swieten, P.; Aalberse, R.C.
Title (up) Evidence for a Fel d I-like molecule in the “big cats” (Felidae species) Type Journal Article
Year 1990 Publication J Allergy Clin Immunol Abbreviated Journal
Volume 86 Issue 1 Pages 107-116
Keywords Adolescence; Adult; Allergens; immunology; Animal; Antibodies; Monoclonal; diagnostic; use; Antibody; Specificity; Carnivora; Cats; Comparative; study; Cross; Reactions; Hair; Histamine; Release; Human; IgE; analysis; IgG; Middle; Age; Radioallergosorbent; Test; methods; Support; Non-U.S.Gov't; browse; us; government; gov't; 240
Abstract In this study, we investigated the cross-reactivity pattern of IgE and IgG4 antibodies to the major feline allergen, Fel d I. We studied the IgE and IgG4 response of 11 cat-allergic patients against Fel d I-like structures in eight members of the Felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. Hair from these “big cats” was collected, extracted, and used in a RAST system and histamine-release test. By means of a RAST-inhibition assay with affinity-purified Fel d I from cat dander, it was established that, in the Felidae species, a Fel d I equivalent is present that reacts with IgE and IgG4 antibodies. We found that all patients had cross-reacting IgE antibodies to seven of the Felidae tested; no IgE antibodies reactive with the caracal were found. Eight of 10 patients with IgG4 antibodies directed to cat dander also had IgG4 antibodies directed to several Felidae species, including the caracal. However, the correlation between the IgE and the IgG4 antibody specificity was low, indicating that, in the case of Fel d I IgE and IgG4, antibodies do not necessarily have the same specificity.
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ISSN 0091-6749 ISBN Medium
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Notes Document Type: eng Approved no
Call Number SLN @ rana @ 157 Serial 233
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Author Karmacharya, D.
Title (up) Field Protocol – Scat Collection for Genetic Analysis Type Miscellaneous
Year 2011 Publication Abbreviated Journal
Volume Issue Pages 1-11
Keywords field protocol, scat collection, scat, genetic analysis, Nepal
Abstract Project funded by Snow Leopard Conservation Grant Program. Center for Molecular Genetics, Nepal.
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Language English Summary Language Original Title
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Notes Approved no
Call Number SLN @ rana @ Serial 1346
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Author Atzeni, L., Wang, J., Riordan, P., Shi, K., Cushman, S. A.
Title (up) Landscape resistance to gene flow in a snow leopard population from Qilianshan National Park, Gansu, China Type Journal Article
Year 2023 Publication Landscape Ecology Abbreviated Journal
Volume Issue Pages
Keywords Landscape genetics · MLPE · Gene flow · Genetic distance · Isolation by distance · Isolation by resistance · Landscape resistance · Snow leopard · Principal component analysis
Abstract Context: The accurate estimation of landscape resistance to movement is important for ecological understanding and conservation applications. Rigorous estimation of resistance requires validation and optimization. One approach uses genetic data for the optimization or validation of resistance models. Objectives We used a genetic dataset of snow leopards from China to evaluate how landscape genetics resistance models varied across genetic distances and spatial scales of analysis. We evaluated whether landscape genetics models were superior to models of resistance derived from habitat suitability or isolation-by-distance.

Methods: We regressed genetically optimized, habitat-based, and isolation-by-distance hypotheses against genetic distances using mixed effect models. We explored all subset combinations of genetically optimized variables to find the most supported resistance scenario for each genetic distance.

Results: Genetically optimized models always out-performed habitat-based and isolation-by-distance hypotheses. The choice of genetic distances influenced the apparent influence of variables, their spatial scales and their functional response shapes, producing divergent resistance scenarios. Gene flow in snow leopards was largely facilitated by areas of intermediate ruggedness at intermediate elevations corresponding to small-to-large valleys within and between the mountain ranges.

Conclusions: This study highlights that landscape genetics models provide superior estimation of functional dispersal than habitat surrogates and suggests that optimization of genetic distance should be included as an optimization routine in landscape genetics, along with variables, scales, effect size and functional response shape. Furthermore, our study provides new insights on the ecological conditions that promote gene flow in snow leopards, which expands ecological knowledge, and we hope will improve conservation planning.
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Notes Approved no
Call Number SLN @ rakhee @ Serial 1720
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Author Jumabay, K., Wegge, P., Mishra, C., Sharma, K
Title (up) Large carnivores and low diversity of optimal prey: a comparison of the diets of snow leopards Panthera uncia and wolves Canis lupus in Sarychat-Ertash Reserve in Kyrgyzstan Type Journal Article
Year 2013 Publication Oryx Abbreviated Journal
Volume Issue Pages 1-7
Keywords Canis lupus, Central Asia, food habits, intraguild competition, Panthera uncia, scat analysis
Abstract In the cold and arid mountains of Central Asia, where the diversity and abundance of wild ungulates

are generally low, resource partitioning among coexisting carnivores is probably less distinct than in prey-rich areas. Thus, similar-sized carnivores are likely to compete for food. We compared the summer diets of snow leopards Panthera uncia and wolves Canis lupus in Sarychat-Ertash Reserve in the Tien-Shan mountains of Kyrgyzstan, based on analysis of genetically confirmed scats. Abundances of

the principal prey species, argali Ovis ammon and Siberian ibex Capra sibirica, were estimated from field surveys. The diets consisted of few species, with high interspecific overlap (Pianka’s index50.91). Argali was the predominant prey, with .50% frequency of occurrence in both snow leopard and wolf scats. This was followed by Siberian ibex and marmots Marmota baibacina. Being largely unavailable, remains of livestock were not detected in any of the scats. In the snow leopard diet, proportions of argali and ibex were in

line with the relative availabilities of these animals in the Reserve. This was in contrast to the diet of wolf, where argali occurred according to availability and ibex was significantly underrepresented. The high diet overlap indicates that the two predators might compete for food when the diversity of profitable, large prey is low. Competition may be more intense in winter, when marmots are not available. Hunting of argali and ibex outside the Reserve may be unsustainable and therefore reduce their abundances over time. This will

affect both predators negatively and intensify competition for food. Reduction in ibex populations will directly affect the snow leopard, and the wolf is likely to be indirectly affected as a result of increased snow leopard predation of argali.
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Call Number SLN @ rakhee @ Serial 1397
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Author Henschel, P.; Ray, J.
Title (up) Leopards in African Rainforests: Survey and Monitoring Techniques Type Miscellaneous
Year 2003 Publication Abbreviated Journal
Volume Issue Pages
Keywords forest leopards; african rainforests; survey; monitoring techniques; lope reserve; gabon; central africa; congo; zaire; field testing; populations; wild meat; relative abundance; density; live-trapping; presence and absense surveys; ad-hoc survey; bushmeat; systematic survey; monitoring; individual identification; tracks; Discriminant Function Analysis; genotyping; scat; Hair; Dna; remote photography; camera trapping; capture rates; Trailmaster; Camtrakker; bait; duikers; pigs; elephant; bongo; okapi; human hunters; 5300
Abstract Monitoring Techniques Forest leopards have never been systematically surveyed in African forests, in spite of their potentially vital ecological role as the sole large mammalian predators in these systems. Because leopards are rarely seen in this habitat, and are difficult to survey using the most common techniques for assessing relative abundances of forest mammals, baseline knowledge of leopard ecology and responses to human disturbance in African forests remain largely unknown. This technical handbook sums up the experience gained during a two-year study of leopards by Philipp Henschel in the Lop‚ Reserve in Gabon, Central Africa, in 2001/2002, supplemented by additional experience from carnivore studies conducted by Justina Ray in southwestern Central African Republic and eastern Congo (Zaire) . The main focus of this effort has been to develop a protocol that can be used by fieldworkers across west and central Africa to estimate leopard densities in various forest types. In developing this manual, Henschel tested several indirect methods to assess leopard numbers in both logged and unlogged forests, with the main effort devoted to testing remote photography survey methods developed for tigers by Karanth (e.g., Karanth 1995, Karanth & Nichols 1998; 2000; 2002), and modifying them for the specific conditions characterizing African forest environments. This handbook summarizes the results of the field testing, and provides recommendations for techniques to assess leopard presence/absence, relative abundance, and densities in African forest sites. We briefly review the suitability of various methods for different study objectives and go into particular detail on remote photography survey methodology, adapting previously developed methods and sampling considerations specifically to the African forest environment. Finally, we briefly discuss how camera trapping may be used as a tool to survey other forest mammals. Developing a survey protocol for African leopards is a necessary first step towards a regional assessment and priority setting exercise targeted at forest leopards, similar to those carried out on large carnivores in Asian and South American forests.
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Publisher Place of Publication Editor Wildlife Conservation Society
Language Summary Language Original Title
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Notes Approved no
Call Number SLN @ rana @ 515 Serial 382
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Author Jackson, R.
Title (up) Linking Snow Leopard Conservation and People-Wildlife Conflict Resolution, Summary of a multi-country project aimed at developing grass-roots measures to protect the endangered snow leopard from herder retribution Type Journal Article
Year 2000 Publication Cat News Abbreviated Journal
Volume 33 Issue Pages 12-15
Keywords livestock-depredation; livestock; pastoralists; herders; Pakistan; Nepal; Tibet; Mongolia; India; protected-areas; parks; reserves; refuge; snow-leopard-incentive-program; economics; tourism; pens; corrals; enclosures; trapping; poisoning; killing; cubs; dens; retribution; behavior; predator; prey; Qomolangma; habitat; feces; fecal-analysis; compensation; Dogs; guard-dogs; religion; conservation; browse; depredation; snow; leopard; incentive; program; fecal; analysis; guard; Dog; 4000
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Notes Full Text at URL Approved no
Call Number SLN @ rana @ 375 Serial 464
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Author Hol, E.H.; Marden, T.
Title (up) Methods for Sampling and Analysis to establish potential exposure of wildlife to persistent contaminants in remote areas Type Conference Article
Year 1994 Publication Abbreviated Journal
Volume Issue Pages 279-287
Keywords ecotoxicological; Himalaya; chemicals; pollution; contaminants; Blood; fur; Hair; scat; excrement; adipose; hair-analysis; browse; analysis; 3300
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Publisher Islt Place of Publication Usa Editor J.L.Fox; D.Jizeng
Language Summary Language Original Title
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Notes Full Text at URLTitle, Monographic: Seventh International Snow Leopard SymposiumPlace of Meeting: ChinaDate of Copyright: 1994 Approved no
Call Number SLN @ rana @ 225 Serial 386
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Author Thapa, K., Schmitt, N., Pradhan, N. M. B., Acharya, H. R., Rayamajhi, S.
Title (up) No silver bullet? Snow leopard prey selection in Mt. Kangchenjunga, Nepal Type Journal Article
Year 2021 Publication Ecology and Evolution Abbreviated Journal
Volume Issue Pages 1-13
Keywords blue sheep, common leopard, fecal, genetic analysis, snow leopard, wolf, yak
Abstract In this study, we investigated the impact of domestic and wild prey availability on snow leopard prey preference in the Kangchenjunga Conservation Area of eastern Nepal-a region where small domestic livestock are absent and small wild ungulate prey are present. We took a comprehensive approach that combined fecal genetic sampling, macro- and microscopic analyses of snow leopard diets, and direct observation of blue sheep and livestock in the KCA. Out of the collected 88 putative snow leopard scat samples from 140 transects (290km) in 27 (4x4km2) sampling grid cells, 73 (83%) were confirmed to be from snow leopard. The genetic analysis accounted for 19 individual snow leopards (10 males and 9 females), with a mean population size estimate of 24 (95% CI: 19-29) and an average density of 3.9 snow leopards/100km2 within 609km2. The total available prey biomass of blue sheep and yak was estimated at 355,236 kg (505 kg yak/km2 and 78kg blue sheep/km2). From the available prey biomass, we estimated snow leopards consumed 7% annually, which comprised wild prey (49%), domestic livestock (45%). and 6% unidentified items. the estimated 47,736 kg blue sheep biomass gives a snow leopard-to-blue sheep ratio of 1:59 on a weight basis. The high preference of snow leopard to domestic livestock appears to be influenced by a much smaller available biomass of wild prey then in other regions of Nepal (e.g., 78kg/km2 in the KCA compared with a range of 200-300 kg/km2 in other regions of Nepal?. Along with livestock insurance scheme improvement, there needs to be a focus on improved livestock guarding, predator-proof corrals as well as engaging and educating local people to be citizen scientists on the importance of snow leopard conservation, involving them in long-term monitoring programs and promotion of ecotourism.
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Call Number SLN @ rakhee @ Serial 1665
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Author Graham, L.H.; Goodrowe, K.L.; Raeside, J.I.; Liptrap, R.M.
Title (up) Non-invasive monitoring of ovarian function in several felid species by measurement of fecal estradiol-17-beta and progestins Type Journal Article
Year 1995 Publication Zoo Biology Abbreviated Journal
Volume 14 Issue 3 Pages 223-237
Keywords Artificial-Breeding-Program; captive-management; Estradiol-17beta; Pregnancy; Progesterone; Progestin; sexual-behavior; genetics; zoo; medicine; veterinary; snow-leopard; feces; fecal-analysis; snow leopard; artificial; breeding; program; captive; management; Estradiol; 17beta; sexual; behavior; browse; snow; leopard; fecal; analysis; 1390
Abstract An extraction and assay procedure to measure fecal estradiol-17-beta and progestin concentrations in several cat species was developed and validated for use for noninvasive monitoring of ovarian function. Fecal samples were collected over a range of 3-20 months from female tigers (three), lions (three), snow leopards (three), cheetahs (two), caracals (two), and domestic cats (five). Samples were extracted with 90% methanol, lipids removed with petroleum ether, and the estradiol and progestins in the methanol measured by radioimmunoassay (RIA). High Performance Liquid Chromatography (HPLC) fractionation and subsequent RIA of the fractions indicated that the estradiol-17-beta antiserum cross-reacted primarily with estradiol-17-beta in the feces of lions and tigers and was assumed to be specific for estradiol-17-beta in the feces of other species as well. However, there were several immunoreactive compounds, presumably progesterone metabolites, excreted in the feces which varied both quantitatively and qualitatively among species. The behavior of tigers, lions, cheetahs, and caracals was visually monitored during the collection period and frequency of sexual behaviors was positively correlated with increases in fecal estradiol in all species observed. The mean fecal estradiol-17-beta peaks were as follows: tigers, 128.0 +- 13.1; lions, 186.0 +- 14.8; snow leopards, 136.7 +- 15.9; cheetahs, 140.9 +- 9.0; caracals, 24.5 +- 4.0; and domestic cats 158.9 +- 19.3 ng/gm. Fecal progestin concentrations rose significantly (P lt 0,001) only after breeding or during pregnancy and were as follows: tigers, 5.6 +- 0.6; lions, 1.9 +- 0.1; cheetahs, 8.4 +- 1.1; and caracals, 2.4 +- 0.4 mu-g/gm. Fecal progestins were elevated for one-half to two-thirds of the gestation length during presumed pseudopregnancy but remained elevated throughout successful pregnancies. These results suggest that ovarian function can be monitored noninvasively in the family Felidae by the measurement of fecal estradiol-17-beta and progestin concentrations.
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Notes Document Type: English Approved no
Call Number SLN @ rana @ 279 Serial 345
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Author Zhirjakov, V.A.
Title (up) On the ecology of the snow leopard in the Zailisky-Alatau (Northern Tien Shan) Type Journal Article
Year 1990 Publication Int Ped Book of Snow Leopards Abbreviated Journal
Volume 6 Issue Pages 25-30
Keywords Tien-Shan; ecology; China; browse; Kazakhstan; Russia; Soviet-Union; distribution; population; prey; behavior; food-habits; scat-analysis; 3240
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Notes Full text available at URL Approved no
Call Number SLN @ rana @ 168 Serial 1078
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