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Author Aruge, S., Batool, H., Khan, F. M., Abbas, F. I., Janjua, S
Title (up) A pilot study�genetic diversity and population structure of snow leopards of Gilgit-Baltistan, Pakistan, using molecular techniques. Type Journal Article
Year 2019 Publication PeerJ Abbreviated Journal
Volume Issue 7672 Pages 1-14
Keywords Population, Genetics, Panthera uncia, Pakistan, Molecular markers
Abstract Background: The Hindu Kush and Karakoram mountain ranges in Pakistan�s northern areas are a natural habitat of the snow leopard (Panthera uncia syn. Uncia uncia) but the ecological studies on this animal are scarce since it is human shy by nature and lives in dif!cult mountainous tracts. The pilot study is conducted to exploit the genetic diversity and population structure of the snow leopard in this selected natural habitat of the member of the wildcat family in Pakistan.

Method: About 50 putative scat samples of snow leopard from !ve localities of Gilgit-Baltistan (Pakistan) along with a control sample of zoo maintained male snow leopard were collected for comparison. Signi!cant quality and quantity of genomic DNA was extracted from scat samples using combined Zhang�phenol�chloroform method and successful ampli!cation of cytochrome c oxidase I gene (190 bp) using mini-barcode primers, seven simple sequence repeats (SSR) markers and Y-linked AMELY gene (200 bp) was done.

Results: Cytochrome c oxidase I gene sequencing suggested that 33/50 (66%) scat samples were of snow leopard. AMELY primer suggested that out of 33 ampli!ed samples, 21 (63.63%) scats were from male and 12 (36.36%) from female leopards. Through successful ampli!cation of DNA of 25 out of 33 (75.75%) scat samples using SSR markers, a total of 68 alleles on seven SSR loci were identi!ed, showing low heterozygosity, while high gene "ow between population.

Discussion: The low gene flow rate among the population results in low genetic diversity causing decreased diversi!cation. This affects the adaptability to climatic changes, thus ultimately resulting in decreased population size of the species.
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Notes Approved no
Call Number SLN @ rakhee @ Serial 1491
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Author Hunter, D.O.; Jackson, R.
Title (up) A Range-Wide Model of Potential Snow Leopard Habitat Type Conference Article
Year 1997 Publication Abbreviated Journal
Volume Issue Pages 51-56
Keywords habitat; Gis; Afghanistan; Bhutan; China; India; Kazakhstan; Kyrgyzstan; Mongolia; Burma; Myanmar; Nepal; Pakistan; Russia; Tajikistan; Uzbekistan; parks; protected-area; kazakstan; browse; protected; area; 2620
Abstract
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Corporate Author Thesis
Publisher Islt Place of Publication Lahore, Pakistan Editor R.Jackson; A.Ahmad
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Full Text at URLTitle, Monographic: Eight International Snow Leopard SymposiumPlace of Meeting: Islamabad, PakistanDate of Copyright: 1997 Approved no
Call Number SLN @ rana @ 313 Serial 396
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Author Fox, J.L.
Title (up) A review of the status and ecology of the snow leopard (Panthera uncia) Type Miscellaneous
Year 1989 Publication Abbreviated Journal
Volume Issue Pages
Keywords status; ecology; distribution; browse; behavior; Russia; China; Mongolia; Soviet-Union; Pakistan; India; Nepal; Afganastan; Bhutan; mating; sexual-behavior; research; surveys; scrapes; sprays; habitat; 2050
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Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
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ISSN ISBN Medium
Area Expedition Conference
Notes Full Text at URL Approved no
Call Number SLN @ rana @ 137 Serial 294
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Author Ahmad, I.; Hunter, D.O.; Jackson, R.
Title (up) A Snow Leopard and Prey Species Survey in Khunjerab National Park, Pakistan Type Conference Article
Year 1997 Publication Abbreviated Journal
Volume Issue Pages 92-95
Keywords Slims; Islt; Wwf; predator; prey; Pakistan; Khunjerab; parks; park; reserve; reserves; refuge; Marco-Polo-sheep; blue-sheep; surveys; survey; transect; sighn; markings; marking; scrape; spray; ibex; tracks; pug marks; feces; livestock; kill; herder; herders; protected-area; blue; sheep; browse; international snow leopard trust; world wildlife fund; marco polo sheep; marco polo; pug; marks; protected area; protected areas; protected; area; areas; 2810
Abstract
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Corporate Author Thesis
Publisher Islt Place of Publication Lahore, Pakistan Editor R.Jackson; A.Ahmad
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Title, Monographic: Eighth International Snow Leopard SymposiumPlace of Meeting: Islamabad, PakistanDate of Copyright: 1997 Approved no
Call Number SLN @ rana @ 300 Serial 42
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Author ud Din, J.
Title (up) Assessing the Status of Snow Leopard in Torkhow Valley, District Chitral, Pakistan: Final Technical Report Type Report
Year 2008 Publication Abbreviated Journal
Volume Issue Pages 1-16
Keywords status; snow; snow leopard; snow-leopard; leopard; valley; chitral; Pakistan; Report; study; prey; Base; conflict; threats; threat; wildlife; sign; transect; surveys; survey; Slims; Data; number; snow leopards; snow-leopards; leopards; Animals; Animal; population; livestock; livestock depredation; livestock-depredation; depredation; area; Case; ungulates; ungulate; Himalayan; himalayan ibex; ibex; rut; using; prey species; prey-species; species; marmot; game; birds; carnivores; carnivore; wolf; wolves; jackal; fox; survival; retaliatory; retaliatory killing; retaliatory-killing; killing; poisoning; poaching; loss; habitat; habitat degradation; habitat-degradation; degradation; grazing; collection; awareness; Gis; map; staff; field; training; conservation; community; distribution; resource; project; network; program
Abstract This study was aimed at assessing the status of Snow leopard, its major prey base, and the extent of human-Snow leopard conflict and major threats to the wildlife in north Chitral (Torkhow valley) Pakistan. Snow leopard occurrence was conformed through sign transect surveys i.e. SLIMS. Based on the data collected the number of Snow leopards in this survey block (1022 Kmý) is estimated to be 2-3 animals. Comparing this estimate with the available data from other parts of the district the population of snow leopard in Chitral district was count to be 36 animals. Livestock depredation reports collected from the area reflect the existence of human-snow leopard conflict and 138 cases were recorded affecting 102 families (in a period of eight years, 2001-2008). Ungulates (Himalayan Ibex) rut season surveys were conducted in coordination with NWFP Wildlife department. A total of 429 animals were counted using direct count (point method) surveys. Other snow leopard prey species recorded include marmot, hare, and game birds. Signs of other carnivores i.e. wolf, jackal, and fox were also noticed. Major threats to the survival of wildlife especially snow leopard reckoned include retaliatory killing (Shooting, Poisoning), poaching, loss of natural prey, habitat degradation (over grazing, fodder and fuel wood collection), lack of awareness, and over population. GIS map of the study area was developed highlighting the area searched for Snow leopard and its prey species. Capacity of the Wildlife Department staff was built in conducting SLIMS and ungulate surveys through class room and on field training. Awareness regarding the importance of wildlife conservation was highlighted to the students, teachers and general community through lectures and distribution of resource materials developed by WWF-Pakistan.
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Notes Project funded by Snow Leopard Network's Snow Leopard Conservation Grant Program. Approved no
Call Number SLN @ rana @ 1065 Serial 978
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Author Khan, J.
Title (up) Availability of snow leopard pelt in Pakistan Type Report
Year 2002 Publication Abbreviated Journal
Volume Issue Pages
Keywords snow leopard, Pakistan, pelt, wildlife trade
Abstract
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Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
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Notes Approved no
Call Number SLN @ rana @ Serial 1246
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Author Ahmad, A.
Title (up) Community-Based Natural Resources Management in Northern Pakistan Type Conference Article
Year 1997 Publication Abbreviated Journal
Volume Issue Pages 148-154
Keywords conservation; livestock; Wwf; Pakistan; herders; herder; snow-leopard; management; Marco-Polo-sheep; grazing; ibex; park; parks; reserve; reserves; refuge; Khunjerab; hunting; hunter; skin; pelt; fur; coat; protected-area; snow leopard; browse; 2950
Abstract
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Corporate Author Thesis
Publisher Islt Place of Publication Lahore, Pakistan Editor R.Jackson and A.Ahmad
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Full text at URLTitle, Monographic: Eighth International Snow Leopard SymposiumPlace of Meeting: Islamabad, IndiaDate of Copyright: 1997 Approved no
Call Number SLN @ rana @ 299 Serial 41
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Author Hussain, I.
Title (up) Conserving Biodiversity through Institutional Diversity: Concept Paper Type Miscellaneous
Year 1999 Publication Abbreviated Journal
Volume Issue Pages
Keywords Iunc; status; distribution; Baltistan; Pakistan; herders; farmers; conservation; killing; poaching; conflict; livestock; predator; prey; retaliatory-killing; economy; corral; capture; trapping; Project-snow-leopard; ecotourism; tourism; compensation; markhor; trekking; browse; retaliatory; 3910
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Notes Full Text at URL Approved no
Call Number SLN @ rana @ 362 Serial 397
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Author Khatoon, R., Hussain, I., Anwar, M., Nawaz, M. A.
Title (up) Diet selection of snow leopard (Panthera uncia) in Chitral, Pakistan Type Journal Article
Year 2017 Publication Turkish Journal of Zoology Abbreviated Journal
Volume Issue 14 Pages 914-923
Keywords Snow leopard, conservation, seasonal variation, diet composition, Chitral, Pakistan
Abstract Snow leopard (Panthera uncia) is an elusive endangered carnivore found in remote mountain regions of Central Asia, with

sparse distribution in northern Pakistan, including Chitral and Baltistan. The present study determined the food habits of snow leopard,

including preferred prey species and seasonal variation in diet. Fifty-six scat samples were collected and analyzed to determine the

diet composition in two different seasons, i.e. summer and winter. Hair characteristics such as cuticular scale patterns and medullary

structure were used to identify the prey. This evidence was further substantiated from the remains of bones, claws, feathers, and other

undigested remains found in the scats. A total of 17 prey species were identified; 5 of them were large mammals, 6 were mesomammals,

and the remaining 6 were small mammals. The occurrence of wild ungulates (10.4%) in the diet was low, while livestock constituted a

substantial part (26.4%) of the diet, which was higher in summer and lower in winter. Mesomammals altogether comprised 33.4% of

the diet, with palm civet (Paguma larvata) as a dominant (16.8%) species, followed by golden marmot (Marmota caudate) (8.8%), which

was higher in winter. There was a significant difference in seasonal variation in domestic livestock and small mammals. The livestock

contribution of 26.4% observed in the present study indicates a significant dependence of the population on livestock and suggests

that the study area is expected to be a high-conflict area for snow leopards. The results of the current study would help improve the

conservation efforts for snow leopards, contributing to conflict resolution and effective management of this endangered cat.
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Notes Approved no
Call Number SLN @ rakhee @ Serial 1459
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Author Jack, R.
Title (up) DNA Testing and GPS positioning of snow leopard (Panthera uncia) genetic material in the Khunjerab National Park Northern Areas, Pakistan Type Report
Year 2008 Publication Abbreviated Journal
Volume Issue Pages 1-15
Keywords project; snow; snow leopard; snow-leopard; leopard; network; conservation; program; Dna; Gps; panthera; panthera uncia; Panthera-uncia; uncia; Khunjerab; Khunjerab-National-Park; national; national park; National-park; park; areas; area; Pakistan; protection; snow leopards; snow-leopards; leopards; local; local people; people; information; number; range; Animals; Animal; study; distribution; management; professional; techniques; capture; use; field; country; China; border; work; art; Gis; Forest; manage; Wwf; maps; map; location; training; research; mountain
Abstract The protection of Snow Leopards in the remote and economically disadvantaged Northern Areas of Pakistan needs local people equipped with the skills to gather and present information on the number and range of individual animals in their area. It is important for the success of a conservation campaign that the people living in the area are engaged in the conservation process. Snow Leopards are elusive and range through inhospitable terrain so direct study is difficult. Consequently the major goals for this project were twofold, to gather information on snow leopard distribution in this area and to train local university students and conservation management professionals in the techniques used for locating snow leopards without the need to capture or even see the animals. This project pioneered the use of DNA testing of field samples collected in Pakistan to determine the distribution of snow leopards and to attempt to identify individuals. These were collected in and around that country's most northerly national park, the Kunjurab National Park, which sits on the Pakistan China border. Though the Northern Areas is not a well developed part of Pakistan, it does possess a number of institutions that can work together to strengthen snow leopard conservation. The first of these is a newly established University with students ready to be trained in the skills needed. Secondly WWF-Pakistan has an office in the main town and a state of the art GIS laboratory in Lahore and already works closely with the Forest Department who manage the national park. All three institutions worked together in this project with WWF providing GIS expertise, the FD rangers, and the university students carrying out the laboratory work. In addition in the course of the project the University of the Punjab in Lahore also joined the effort, providing laboratory facilities for the students. As a result of this project maps have been produced showing the location of snow leopards in

two areas. Preliminary DNA evidence indicates that there is more than one animal in this

relatively small area, but the greatest achievement of this project is the training and

experience gained by the local students. For one student this has been life changing. Due to

the opportunities provided by this study the student, Nelofar gained significant scientific

training and as a consequence she is now working as a lecturer and research officer for the

Center for Integrated Mountain Research, New Campus University of the Punjab, Lahore

Pakistan
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Notes Project funded by Snow Leopard Network's Snow Leopard Conservation Grant Program. Approved no
Call Number SLN @ rana @ 1067 Serial 427
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