Abstract: Liver tissues from 126 captive cheetah were evaluated by light microscopy and histochemistry; eight animals were evaluated by electron microscopy. The main hepatic lesion, a vascular lesion resembling veno- occlusive disease (VOD) of the liver and characterized by subendothelial fibrosis and proliferation of smooth muscle-like cells in the central veins, was seen in 60% of the sexually mature cheetah. Although this hepatic vascular lesion was seen in cheetah as young as 1 year of age, the most severe lesions, usually associated with liver failure, were found in cheetah between the ages of 6 and 11. There was no sex predisposition, and in approximately 40% of the VOD cases, liver disease was not suspected clinically or at necropsy. VOD was found in other felidae, especially in the snow leopard. High levels of vitamin A in livers, as well as in diets of the cheetah, could be a contributing factor in the development of VOD in some groups of cheetah.

Abstract: Snow leopard is rare and endangered species. At present it is met in Tien Shan and the spurs: in the ridges of Pskem, Ugam, Karjantau, Talas, Kyrgyz, Zailiyskiy, Ketmene, Kungei Alatau, Terskey Alatau, as well as Jungar Alatau, Tarbagatai, Saure, and Altai. In 19th century, snow leopard used to be met in the Karatau ridge (the Syrdarya ridge). Over the last two decades population of snow leopard reduced due to increased development of mountainous areas and reduction of wild animal populations (ibex, argali, morals, marmots, etc.). In Kazakhstan, snow leopard is protected in the Aksu-Djabagly and Alma-Ata nature reserves.

Abstract: Status: rare (Category III). Distribution: Tien Shan mountains, Tarbagatai, Saur and Altai mountains. Total number in Kazakhstan does not exceed 200 individuals. The main threats are poaching and reduction number of preys. In Almaty Zoo captive breeding was successful in 1976 and 1985. Snow leopard is protected in Aksu Jabagly, Almaty and Markakol nature reserves. To ensure the survival of the species it is necessary to establish a reserve in Dzhungar mountains and to improve protection in existing nature reserves.

Abstract: An extraction and assay procedure to measure fecal estradiol-17-beta and progestin concentrations in several cat species was developed and validated for use for noninvasive monitoring of ovarian function. Fecal samples were collected over a range of 3-20 months from female tigers (three), lions (three), snow leopards (three), cheetahs (two), caracals (two), and domestic cats (five). Samples were extracted with 90% methanol, lipids removed with petroleum ether, and the estradiol and progestins in the methanol measured by radioimmunoassay (RIA). High Performance Liquid Chromatography (HPLC) fractionation and subsequent RIA of the fractions indicated that the estradiol-17-beta antiserum cross-reacted primarily with estradiol-17-beta in the feces of lions and tigers and was assumed to be specific for estradiol-17-beta in the feces of other species as well. However, there were several immunoreactive compounds, presumably progesterone metabolites, excreted in the feces which varied both quantitatively and qualitatively among species. The behavior of tigers, lions, cheetahs, and caracals was visually monitored during the collection period and frequency of sexual behaviors was positively correlated with increases in fecal estradiol in all species observed. The mean fecal estradiol-17-beta peaks were as follows: tigers, 128.0 +- 13.1; lions, 186.0 +- 14.8; snow leopards, 136.7 +- 15.9; cheetahs, 140.9 +- 9.0; caracals, 24.5 +- 4.0; and domestic cats 158.9 +- 19.3 ng/gm. Fecal progestin concentrations rose significantly (P lt 0,001) only after breeding or during pregnancy and were as follows: tigers, 5.6 +- 0.6; lions, 1.9 +- 0.1; cheetahs, 8.4 +- 1.1; and caracals, 2.4 +- 0.4 mu-g/gm. Fecal progestins were elevated for one-half to two-thirds of the gestation length during presumed pseudopregnancy but remained elevated throughout successful pregnancies. These results suggest that ovarian function can be monitored noninvasively in the family Felidae by the measurement of fecal estradiol-17-beta and progestin concentrations.

Abstract: Considerable efforts have been devoted to conserving the snow leopard Panthera uncia in recent years, but progress has inevitably been slow due to the difficulties of studying a sparsely distributed, secretive and endangered species in often isolated mountainous terrain. Although knowledge about the species overall distribution in the highlands of Central Asia still remains fragmenatry, it is important to briefly examine all the available information in order to review measures taken to date to conserve the species through the protected areas network. The purpose of this paper is to examine the distribution and status of protected areas inhabited or visited by snow leopard in relation to the species' distribution and highlight deficiences in the present network.