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Mukherjee, S., Ashalakshmi, C. N., Home, C., Ramakrishnan, U. (2010). SAhonrt Reepvoartluation of the PCR-RFLP technique to aid molecular-based monitoring of felids and canids in India. BMC Research Notes, 3, 159–166.
Abstract: Background: The order Carnivora is well represented in India, with 58 of the 250 species found globally, occurring here. However, small carnivores figure very poorly in research and conservation policies in India. This is mainly due to
the dearth of tested and standardized techniques that are both cost effective and conducive to small carnivore studies in the field. In this paper we present a non-invasive genetic technique standardized for the study of Indian felids and canids with the use of PCR amplification and restriction enzyme digestion of scat collected in the field.
Findings: Using existing sequences of felids and canids from GenBank, we designed primers from the 16S rRNA region of the mitochondrial genome and tested these on ten species of felids and five canids. We selected restriction enzymes that would cut the selected region differentially for various species within each family. We produced a restriction digestion profile for the potential differentiation of species based on fragment patterns. To test our technique, we used felid PCR primers on scats collected from various habitats in India, representing varied environmental conditions. Amplification success with field collected scats was 52%, while 86% of the products used for restriction digestion could be accurately assigned to species. We verified this through sequencing. A comparison of costs across the various techniques currently used for scat assignment showed that this technique was the most practical and cost effective.
Conclusions: The species-specific key developed in this paper provides a means for detailed investigations in the future that focus on elusive carnivores in India and this approach provides a model for other studies in areas of Asia
where many small carnivores co-occur.
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Sarkar, P., Takpa, J., Ahmed, R., Tiwari, S. K., Pendharkar, A., ul-Haq, S., Miandad, J., Upadhyay, A., Kaul, R. (2008). Mountain Migrants. Survey of Tibetan Antelope (Pantholops hodgsonii) and Wild Yak (Bos grunniens) in Ladakh, Jammu & Kashmir, India. India.
Abstract: The Tibetan antelope (Pantholops hodgsonii), locally called chiru, is mainly confined to the Tibetan plateau in China. A small population migrates into Chang Thang in eastern Ladakh in the state of Jammu and Kashmir in India. The chiru has a geographical range extending approximately 1,600 km across the Tibetan Plateau, with an eastern limit near Ngoring Hu (Tibet Autonomous Region) and a western limit in Ladakh (India). Large-scale hunting for wool and meat has resulted in a decline of its population and only an estimated 75,000 individuals of this species survive in the world today. Its status in India has not been studied in any detail, although sporadic spot surveys have been done in the past. Similarly, very little information is available on status of wild yak (Bos grunniens), the progenitor (closest ancestor) of the domestic yak in India. The animal is distributed mainly in the highlands of the Tibetan plateau including the Qinghai province, Tibetan and Xinjiang autonomous regions and the Quilian mountains in the Gansu province. Small nomadic isolated populations are reported from Ladakh in Jammu and Kashmir (J&K), and even smaller numbers occasionally from Himachal Pradesh, Uttarakhand, Sikkim and Arunachal Pradesh in India. To obtain further information primarily about these two species, the Department of Wildlife Protection, Jammu & Kashmir (DWP) along with the Wildlife Trust of India (WTI) and the Indian Army initiated surveys in Ladakh in the years 2005 and 2006. Surveys were conducted in the Chang Thang and Karakoram Wildlife Sanctuaries of Ladakh in Jammu & Kashmir. The Chang Chenmo (Chang Thang) area lies in the eastern part of Ladakh just north of the famous Pangong Lake, while the Karakoram WLS lies in the north-eastern part of Ladakh, south of the Karakoram Pass. The team found 250 – 300 chiru in the Karakoram area in addition to other mammal species. Both male and female chiru were sighted here between altitudes of 4735 m and 5336 m. A total of 230 individuals were sighted (after deleting double counts) in the year 2005 and 45 individuals in 2006. Based on this, it is estimated that between 250-300 individuals occur in this area. Mean group size of chiru was 4.66±0.435 and varied between one to 34 individuals during 2005, and 4.5 ± 2.77 (SE) during 2006. Apart from chiru, other species encountered from the area includes Tibetan wolf (Canis lupus chanco), red fox (Vulpes vulpes), pale or mountain weasel (Mustela altaica), snow leopard (Uncia uncia), Ladakh urial (Ovis vignei), blue sheep (Pseudois nayaur), woolly hare (Lepus oiostolus), Ladakh pika (Ochotona ladacensis), Royle's pika (Ochotona roylei), Nubra pika (Ochotona nubrica), plateau pika (Ochotona curzoniae), Stoliczka's mountain vole (Alticola stoliczkanus) and silvery mountain vole (Alticola argentatus).
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Sharma, R., Dutta, T. (2005). Sighting of Lynx (Lynx lynx isabellinus) in Hemis National Park, Ladakh. Zoos's Print, XX(4), 14.
Abstract: We had a good sighting of two adult and one sub adult lynx together, near Ganda-la
base which is at an elevation of 4900 meter, in Hemis National Park, Ladakh, during our fieldwork on Snow leopards in February 2004.The two adults lynx were moving closer and following each other, while the sub-adult lynx was a little far from the adults.
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Hongfa, X. and K., C. (2006). The State of Wildlife Trade in China. Information on the trade in wild animals and plants in China 2006..
Abstract: Welcome to the first edition of The State of Wildlife Trade in China. This publication takes a broad look at wildlife trade over the past year, particularly concerning the impact of China's consumption on globally important biodiversity 'hotspots'. The focus of The State of Wildlife Trade in China is on emerging trends in China's wildlife trade and up-to-date reviews of work to stop illegal wildlife trade and support sustainable trade. The lead story in this issue is the illegal trade in Tigers and other Asian big cats. During 2006, surveys continued to document this illegal trade, as well as highlight opportunities for action. Other stories in this issue give updates on trade in reef fishes from Southeast Asia's 'Coral Triangle' and in timber from the forests of the Russian Far East, Borneo, and East Africa. China's wildlife trade presents both challenges and opportunities. This annual report aims to provide current information about wildlife trade in China and to provide avenues for involvement in China's conservation community. It is part of TRAFFIC's on-going commitment to turn information into action.
Keywords: clouded leopard, economy, illegal killing, leopard, Neofelis nebulosa, Panthera pardus, Panthera tigris, poaching, policy, snow leopard, tiger, trade, TRAFFIC, Uncia uncia
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Froede, K. and J., R. (2001). Snow Leopard Manual Field Study Techniques for the Kingdom Nepal. Kathmandu, Nepal: WWF Nepal.
Abstract: The publication of this manual aims sharing and facilitating the study on snow leopard and its prey species among mid-level professionals interested in conducting fieldwork on their own. The manual is derived from the 1996 “Snow Leopard Survey and Conservation Handbook” written by Dr. Rodney Jackson and Dr. Don Hunter and published by International Snow Leopard Trust (ISLT) based in seatle, Washington, USA. The first section introduces the topic, the second and third section deal with presence/ absence and abundance survey methods. The various survey-froms with instructions are given in the annexes.
Keywords: analysis, census, data, field work, forms, manual, method, methods, monitoring, research, signs, snow leopard, survey, techniques, transects, Uncia uncia
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Zhatkanbayev, A., Dosov, N. (2009). Natural death of a snow leopard close to Almaty megapolis. In Teriofauna of Qazaqstan and neighboring countries (the materials of international science conference “The problems of research, conservation and sustainable use for teriofauna of Qazastan and neighboring countries” (pp. 168–170). Kazakhstan.
Abstract: About natural dead case of Snow leopard close to Almaty megapolis.
Published in special edition as named “Teriofauna of Qazaqstan and neighboring countries (the materials of international science conference ”The problems of research, conservation and sustainable use for teriofauna of Qazastan and neighboring countries“, November, 15-16, 2009, Almaty)” under supervision of the Institute of Zoology, Center of Biological Research, Committee of Science, Ministry of Education and Science, The Republic of Qazaqstan.
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Sharma, S., Thapa, K., Chalise, M., Dutta, T., Bhatnagar, Y.V., McCarthy, T. (2006). The snow leopard in Himalaya: A step towards their conservation by studying their distribution, marking habitat selection, coexistence with other predators, and wild prey-livestock-predator interaction. Conservation Biology in Asia, , 184–196.
Abstract: Snow leopard (Uncia uncial) is a flagship species of the Himalaya. Very few studies have been done on the ecology of this species in the Himalaya. This paper presents an overview of four studies conducted on snow leopards in Nepal and India, dealing with various aspects of snow leopard ecology including their status assessment, making behaviour, habitat selection, food habits, and impact on livestock. The information generated by these studies is useful in planning effective conservation and management strategies for this endangered top predator of high mountains.
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Sharma, S., Dutta, T., Bhatnagar, Y.V. (2006). Marking site selection by free-ranging snow leopard (Uncia uncia). Conservation Biology in Asia, , 197–213.
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International Snow Leopard Trust. Snow Leopards. China.
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Anonymous. (1986). Toward a free-ranging recovery plan.
Abstract: This draft is a first attempt to develop a Snow Leopard Recovery Plan, for consideration at the Fifth International Snow Leopard Symposium. It is intended as a working base for agencies responsible for snow leopard conservation, research and management. The plan, when thoroughly reviewed and revised, will provide more accurate estimates of snow leopard status and threats, and recommendations concerning actions necessary for the maintenance, enhancement and recovery of the snow leopard in its original habitat.
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