Abstract: The existence of a trans-boundary population of the snow leopard (Panthera uncia) that inhabits the massifs of Tsagaanshuvuut (Mongolia) – Tsagan-Shibetu (Russia) was determined through non-invasive genetic analysis of scat samples and by studying the structure of territory use by a collared female individual. The genetic analysis included species identification of samples through sequencing of a fragment of the cytochrome b gene and individual identification using a panel of 8 microsatellites. The home range of a female snow leopard marked with a satellite Global Positioning System (GPS) collar was represented by the minimum convex polygon method (MCP) 100, the MCP 95 method and the fixed kernel 95 method. The results revealed insignificant genetic differentiation between snow leopards that inhabit both massifs (minimal fixation index [FST]), and the data testify to the unity of the cross-border group. Moreover, 5 common individuals were identified from Mongolian and Russian territories. This finding clearly shows that their home range includes territories of both countries. In addition, regular movement of a collared snow leopard in Mongolia and Russia confirmed the existence of a cross-border snow leopard group. These data support that trans-boundary conservation is important for snow leopards in both countries. We conclude that it is crucial for Russia to study the northern range of snow leopards in Asia.

Abstract: Assessments of biodiversity status are needed to
track trends, and the IUCN Red List has become the accepted
global standard for documenting the extinction
risk of species. Obtaining robust data on population size is
an essential component of any assessment of a species� status,
including assessments for the IUCN Red List. Obtaining
such estimates is complicated by methodological and
logistical issues, which are more pronounced in the case of
cryptic species, such as the snow leopard Panthera uncia.
Estimates of the total population size of this species have,
to date, been based on little more than guesstimates, but a
comprehensive summary of recent field research indicates
that the conservation status of the snow leopard may be
less dire than previously thought. A revised categorization,
from Endangered to Vulnerable, on the IUCN Red List was
proposed but met some opposition, as did a recent, similar
recategorization of the giant panda Ailuropoda melanoleuca.
Possible factors motivating such attitudes are discussed.
Downlisting on the IUCN Red List indicates that the species
concerned is further from extinction, and is always to be
welcomed, whether resulting from successful conservation
intervention or improved knowledge of status and trends.
Celebrating success is important to reinforce the message
that conservation works, and to incentivize donors.

Abstract: Molecular markers that can effectively identify noninvasively collected samples and provide genetic
information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many
noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the
domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard
populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and
population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers
facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.
endangered species

Abstract: While the world is becoming increasingly interconnected and interdependent, physically and culturally, the wildlife of remote mountain regions is being affected both positively and negatively by such interconnectedness. In the case of snow leopards, the conservation impact has been largely, and rather unexpectedly, positive: Species-focused conservation projects, such as Project Snow Leopard (PSL) in
Gilgit-Baltistan, remain mainly externally driven initiatives. PSL, initiated as a small pilot project in 1998, has relied on an approach that includes the use of an insurance scheme, the deployment of mitigation measures, and the empowerment of local governance. This approach has been successful in
reducing the conflict with snow leopards and has built greater tolerance toward them. PSL is managed by local communities and cofinanced by them. PSL communities throughout the region are bearing the burden of carnivore conservation, and they are unwittingly subsidizing their populations by ‘‘feeding’’
them their livestock even though they are an economic threat to them. In this article, we argue that external intervention in the form of efforts that help alleviate the consequences of conflict through local empowerment have had a positive impact on the local mountain societies. We also show that such interventions have resulted in tangible conservation results, with the number of snow leopards staying at least stable. Our experience also shows that while the incentive component is critical, it is also part of a larger approach—one that includes developing and supporting local governance structures, improving access to education, and offering a range of tools to reduce the conflict that can be implemented
locally. Finally, we suggest that investing in this approach— one that recognizes the species and local-context complexities surrounding the implementation of conservation incentives—can continue to inform international practices and guidelines for reducing human–wildlife conflicts worldwide.

Abstract: The endangered snow leopard (Panthera uncia) is widely but sparsely distributed throughout the mountainous regions of central Asia. Detailed information on the status and abundance of the snow leopard is limited because of the logistical challenges faced when working in the rugged terrain it occupies, along with its secretive nature. Camera-trapping and noninvasive genetic techniques have been used successfully to survey this felid. We compared noninvasive genetic and camera-trapping snow leopard surveys in the Gobi Desert of Mongolia. We collected 180 putative snow leopard scats from 3 sites during an 8-day period along 37.74 km of transects. We then conducted a 65-day photographic survey at 1 of these sites, approximately 2 months after scat collection. In the site where both techniques were used noninvasive genetics detected 5 individuals in only 2 days of fieldwork compared to 7 individuals observed in the 65-day camera-trapping session. Estimates of population size from noninvasive genetics ranged between 16 and 19 snow leopards in the 314.3-km2 area surveyed, yielding densities of 4.9–5.9 individuals/100 km2. In comparison, the population estimate from the 65-day photographic survey was 4 individuals (adults only) within the 264-km2 area, for a density estimate of 1.5 snow leopards/100 km2. Higher density estimates from the noninvasive genetic survey were due partly to an inability to determine age and exclude subadults, reduced spatial distribution of sampling points as a consequence of collecting scats along linear transects, and deposition of scats by multiple snow leopards on common sites. Resulting differences could inflate abundance estimated from noninvasive genetic surveys and prevent direct comparison of densities derived from the 2 approaches unless appropriate adjustments are made to the study design.

Abstract: Under this grant awarded by The Leonard X. Bosack and Bette M. Kruger Foundation through the auspices of Cat Action Treasury, SLC set out to accomplish the following outcomes in our Stewardship Program:
As of July 1, 2003 we completed the following major activities:
* Tested and compared different remote sensor and camera configurations to determine which is most reliable at “capturing” passing snow leopards;
* Investigated sampling strategies and camera trap placement with respect to snow leopard travel patterns and marking behavior;
* Compared different survey methods: direct (non-invasive capture of photos and DNA material contained in hairs), and indirect (sign transects and presence/absence surveys under the standard SLIMS protocol);
* Assessed the attitude of local people toward snow leopards, wolves and other wildlife as well as their perceptions of benefits and costs associated with the Hemis National Park, in order to craft more effective conservation and park management measures.
These activities mesh with SLC’s ongoing program of predator-proofing livestock corrals in settlements of the Hemis National Park, as well as outside protected areas (including Zanskar, Lingshed and Kanji. For each village’s corrals that are improved, we estimate that five or more snow leopards are saved from retaliatory killing by shepherds who lose valuable livestock.
Our program in assisting villagers to gain supplementary income from tourism-related activities is gaining strength, with trainings in 10 settlements this spring. SLC brought staff of KCC, the Khangchenjunga Conservation Committee, a local NGO based in Sikkim to assist in the skills training and to exchange ideas and experiences from other areas.
Following on from the groundwork laid during the first Bosack-Kruger grant to SLC, we launched a major initiative in collaboration with the Mountain Institute, the Himalayan Homestay program. Funding for this is being provided by UNESCO.
For further information on these and other snow leopard conservation efforts, see our newly designed web-page.
The following paragraphs summarize our accomplishments to date, supported by this grant. For detailed information, please consult the following reports, which are being mailed under separate cover:
1) “Local People’s Attitudes toward Wildlife Conservation in Hemis National Park with Special Reference to the Conservation of Large Predators” (prepared by Rodney Jackson, Rinchen Wangchuk and Jigmet Dadul)
2) “Non-Invasive Monitoring to Support Stewardship of Snow Leopards and Their Prey:
Evaluation of Remote Camera Traps for Censusing Snow Leopards” (prepared by Rodney Jackson and Jerry Roe).

Abstract: While the SLC is just two years old, our twenty years of field experience, numerous contacts and well-established professional relationships have enabled us to work as a mature organization. The Wildlife Conservation Network (WCN) has been instrumental in building awareness of the SLC within the community, and in raising $85,000 in individual donations and merchandise sales which comprised 46% of our 2002 operating budget. With our overhead costs kept to a minimum and being substantially underwritten, donor dollars can be used directly and effectively in the field. With our donors and volunteers committed to helping us build our sustainability, we look forward to even greater success in saving snow leopards in partnership with local people.

Abstract: The snow leopard (Panthera uncia) inhabits the high, remote mountains of Pakistan from where very little information is available on prey use of this species. Our study describes the food habits of the snow leopard in the Himalayas and Karakoram mountain ranges in Baltistan, Pakistan. Ninety-five putrid snow leopard scats were collected from four sites in Baltistan. Of these, 49 scats were genetically confirmed to have originated from snow leopards. The consumed prey was identified on the basis of morphological characteristics of hairs recovered from the scats. It was found that most of the biomass consumed (70%) was due to domestic livestock viz. sheep (23%), goat (16%), cattle (10%), yak (7%), and cattle–yak hybrids (14%). Only 30% of the biomass was due to wild species, namely Siberian ibex (21%), markhor (7%), and birds (2%). Heavy predation on domestic livestock appeared to be the likely cause of conflict with the local inhabitants. Conservation initiatives should focus on mitigating this conflict by minimizing livestock losses.

Abstract: In the Nepalese Himalaya, conflict with rural communities due to livestock predation to large carnivores like snow leopard, common leopard, wolf and wild dog has risen sharply in recent years. This increase is attributed to a number of factors, including implementation and enforcement of wildlife protection laws (which have permitted a recovery in carnivore numbers), the creation of protected areas (which serve as refuges from which predators can populate the surrounding area), the depletion of natural prey due to poaching and loss of habitat, and lax livestock herding practices. However, little information is presently available upon which to design remedial programs. U.S. AID provided research funding for an in-depth assessment of snow leopard predation in the Annapurna Conservation Area (ACAP), an new innovative approach to nature conservation. Baseline information on livestock numbers and mortality were gathered during household interviews, followed by field surveys to assess animal husbandry systems, map pastures, establish periods of use and estimate stocking rates, and to characterize habitat using randomly located plots. Data substantiate the existence of depredation “hotspots”, where high loss occurs, in some cases exceeding 14% to 20% of the livestock population over a short period. Losses varied seasonally, and from year to year. Small-bodied stock like goat and sheep were more vulnerable than large-bodied stock like yak, although horses were especially vulnerable. Factors most closely associated with predation included lack of guarding (or very lax supervision), especially during the daytime, and repeated use of pastures where livestock depredators were known to be actively hunting. Herders usually reacted to repeated depredation incidents by attempting to trap or shoot the suspected culprit until losses declined to an acceptable level. As large carnivore populations become increasingly fragmented and genetically isolated, new management strategies are urgently needed, especially within the buffer zones and intervening corridors between separated parks and reserves. People reside within nearly all Himalayan protected areas, and such issues as loss of livestock and competition between wildlife and livestock cannot be avoided. A plan is offered for alleviating livestock loss in the Annapurna Conservation Area that involves local institutions in decision-making, rewards sound husbandry practices, strengthens indigenous institutions, without further eroding ACAP’s unique biological diversity and diverse carnivore population. The authors believe these measures and ideas could be fruitfully extended to other parts of the Himalaya.