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| Author | Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. | ||||
| Title | Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces | Type | Journal Article | ||
| Year | 1994 | Publication | Biol Reprod | Abbreviated Journal | |
| Volume | 51 | Issue | 4 | Pages | 776-786 |
| Keywords | Animal; Carbon; Radioisotopes; Carnivora; Cats; Chromatography; High; Pressure; Liquid; Comparative Study; Estradiol; metabolism; Estrone; feces; chemistry; Female; Ovary; physiology; Pregnancy; Progesterone; Pseudopregnancy; Support; Non-U.S.Gov't; browse; non; government; gov't; us; 170 | ||||
| Abstract | Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species. | ||||
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| ISSN | 0006-3363 | ISBN | Medium | ||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 251 | Serial | 198 | ||
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| Author | Sundberg, J.P.; Van Ranst, M.; Montali, R.; Homer, B.L.; Miller, W.H.; Rowland, P.H.; Scott, D.W.; England, J.J.; Dunstan, R.W.; Mikaelian, I.; Jenson, A.B. | ||||
| Title | Feline papillomas and papillomaviruses | Type | Journal Article | ||
| Year | 2000 | Publication | Vet Pathol | Abbreviated Journal | |
| Volume | 37 | Issue | 1 | Pages | 1-10 |
| Keywords | Animal; Antibodies; Monoclonal; Carnivora; pathology; virology; Cats; Dna; Viral; chemistry; Epitope; Mapping; veterinary; Female; Immunohistochemistry; Lions; Male; Microscopy; Electron; Papilloma; Papillomavirus; Papovaviridae; Infections; skin; neoplasms; Tongue; browse; 120 | ||||
| Abstract | Papillomaviruses (PVs) are highly species- and site-specific pathogens of stratified squamous epithelium. Although PV infections in the various Felidae are rarely reported, we identified productive infections in six cat species. PV-induced proliferative skin or mucous membrane lesions were confirmed by immunohistochemical screening for papillomavirus-specific capsid antigens. Seven monoclonal antibodies, each of which reacts with an immunodominant antigenic determinant of the bovine papillomavirus L1 gene product, revealed that feline PV capsid epitopes were conserved to various degrees. This battery of monoclonal antibodies established differential expression patterns among cutaneous and oral PVs of snow leopards and domestic cats, suggesting that they represent distinct viruses. Clinically, the lesions in all species and anatomic sites were locally extensive and frequently multiple. Histologically, the areas of epidermal hyperplasia were flat with a similarity to benign tumors induced by cutaneotropic, carcinogenic PVs in immunosuppressed human patients. Limited restriction endonuclease analyses of viral genomic DNA confirmed the variability among three viral genomes recovered from available frozen tissue. Because most previous PV isolates have been species specific, these studies suggest that at least eight different cat papillomaviruses infect the oral cavity (tentative designations: Asian lion, Panthera leo, P1PV; snow leopard, Panthera uncia, PuPV-1; bobcat, Felis rufus, FrPV; Florida panther, Felis concolor, FcPV; clouded leopard, Neofelis nebulosa, NnPV; and domestic cat, Felis domesticus, FdPV-2) or skin (domestic cat, F. domesticus, FdPV-1; and snow leopard, P. uncia, PuPV-2). | ||||
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| ISSN | 0300-9858 | ISBN | Medium | ||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 385 | Serial | 948 | ||
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| Author | The Snow Leopard Conservancy | ||||
| Title | A Learning Tour of the CBN (Corbett, Nainital and Binsar) Eco-tourism Initiative Sites by Villagers from Hemis National Park and the Surrounding Area (18-28th November 2002) | Type | Report | ||
| Year | 2002 | Publication | Abbreviated Journal | ||
| Volume | SLC Field Document Series No 5 | Issue | Pages | ||
| Keywords | Ladakh; Himalayas; Himalaya; Karakoram; mountains; mountain; landscape; tourists; trans-himalayan; transhimalayan; biodiversity; home; snow; snow leopard; snow-leopard; leopard; tourism; number; ecotourism; 80; conservation; traditional; local; community; Culture; income; people; leh; travel; rural; Snow Leopard Conservancy; ecotourism activities; ecotourism-activities; activities; activity; Hemis; national; national park; National-park; park; livelihood; loss; livestock; Animals; Animal; local people; NGO's; eco-tourism; villagers; area | ||||
| Abstract | Ladakh lies between the Great Himalayas and the formidable Karakoram mountains. Its unique landscape and rich cultural heritage have been a great attraction to tourists all over the world. Apart from its uniqueness it has a rich Trans-Himalayan bio-diversity and is home to the rare and elusive snow leopard. It opened to tourism in 1974 with a handful of tourists and has gone up to the present number of about 18,000 visitors annually. Ecotourism started in Ladakh in mid 80s in the form of conservation of traditional architecture when local communities realized the importance of their rich culture and traditions being valued by the visiting tourists. However, while tourism became a major source of income to people in Leh, most of the benefits stayed with outside (Delhi) based travel agents thus leaving out the rural masses. During the last three years Snow Leopard Conservancy and The Mountain Institute have been initiating ecotourism activities with local communities in the Hemis National Park as an alternate livelihood and an indirect way to compensate losses of livestock from predatory animals. However, local people while venturing into such new initiatives have tended to be like blind men that are being led by NGO's so that they do not stumble along their paths. |
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| Publisher | Place of Publication | Leh, Ladakh, India | Editor | Wangchuk, R.; Dadul, J. | |
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ 1025 | Serial | 963 | ||
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| Author | The Snow Leopard Conservancy | ||||
| Title | Local People's Attitudes toward Wildlife Conservation in the Hemis National Park, with Special Reference to the Conservation of Large Predators | Type | Report | ||
| Year | 2003 | Publication | Abbreviated Journal | ||
| Volume | 7 | Issue | Pages | 1-29 | |
| Keywords | local; local people; people; attitudes; attitude; wildlife; conservation; Hemis; national; national park; National-park; park; large; large predators; predators; predator; field; Jackson | ||||
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| Publisher | Place of Publication | Sonoma, California | Editor | ||
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| Notes | SLC Field Series Document No 7. Prepared by R. Jackson, R. Wangchuk, and J. Dadul. Sonoma, California. | Approved | no | ||
| Call Number | SLN @ rana @ 1012 | Serial | 964 | ||
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