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Author Trepanier, L.A.; Cribb, A.E.; Spielberg, S.P.; Ray, K.
Title Deficiency of cytosolic arylamine N-acetylation in the domestic cat and wild felids caused by the presence of a single NAT1-like gene Type Journal Article
Year 1998 Publication Pharmacogenetics Abbreviated Journal
Volume 8 Issue 2 Pages 169-179
Keywords Acetylation; Amino; Acid; Sequence; Animal; Arylamine; N-Acetyltransferase; metabolism; Base; Blotting; Southern; Carnivora; genetics; Cats; Cytosol; enzymology; Dna; Human; Isoenzymes; Liver; Molecular; Data; Polymerase; Chain; Reaction; Rabbits; Homology; Nucleic Acid; Substrate; Specificity; Support; U.S.Gov't; P.H.S.; browse; nucleic; us; government; 130
Abstract The purpose of this study was to determine the molecular basis for a relative deficiency in the cat of cytosolic arylamine N- acetyltransferase (NAT), an enzyme family that is important in the metabolism of xenobiotics and that normally consists of at least two related enzymes, NAT1 and NAT2. N-acetyltransferase in feline liver showed high affinity (mean Km = 2.1 microM) for p-aminobenzoic acid, an NAT1 selective substrate in humans and rabbits, but showed a very poor affinity (mean Km > 10 mM) for sulfamethazine, an NAT2 selective substrate in humans and rabbits. Immunoreactive N-acetyltransferase was detected in feline liver, bladder and colon using an NAT1-specific antipeptide antibody, but was not detected in any tissues using an NAT2- specific antibody. Southern blot analysis of genomic DNA demonstrated a single band in domestic cats using each of six restriction digests; single bands were also found on Southern blot analysis of six wild felids. The deduced amino acid sequence of the central portion of feline N-acetyltransferase, obtained by polymerase chain reaction amplification in both domestic cats and seven wild felids (lion, tiger, lynx, snow leopard, bobcat, Asian leopard cat and cheetah), contained three residues, Phe125, Arg127, and Tyr129, which determine NAT1-like substrate specificity in humans. These results support the conclusion that cytosolic arylamine N-acetylation activity is low in the cat because of the presence of a single N-acetyltransferase that has substrate specificity, immunogenicity and sequence characteristics similar to human NAT1, and that the unusual presence of only a single N- acetyltransferase gene appears to be a family wide trait shared by other felids.
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Publisher Place of Publication Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN 0960-314x ISBN Medium
Area Expedition Conference
Notes Document Type: eng Approved no
Call Number SLN @ rana @ 345 Serial (up) 968
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Author Warren E.Johnson, E.E.
Title The Late Miocene Radiation of Modern Felidae: A Genetic Assessment Type Miscellaneous
Year 2006 Publication Science Abbreviated Journal
Volume 311 Issue Pages 73-77
Keywords classification; divergence; Dna; Felidae; genetics; Miocene; mitochondrial; phylogeny; radiation; species; taxonomic
Abstract Modern felid species descend from relatively recent (<11 million years ago) divergence and

speciation events that produced successful predatory carnivores worldwide but that have

confounded taxonomic classifications. A highly resolved molecular phylogeny with divergence dates

for all living cat species, derived from autosomal, X-linked, Y-linked, and mitochondrial gene

segments (22,789 base pairs) and 16 fossil calibrations define eight principal lineages produced

through at least 10 intercontinental migrations facilitated by sea-level fluctuations. A ghost lineage

analysis indicates that available felid fossils underestimate (i.e., unrepresented basal branch

length) first occurrence by an average of 76%, revealing a low representation of felid lineages

in paleontological remains. The phylogenetic performance of distinct gene classes showed that

Y-chromosome segments are appreciably more informative than mitochondrial DNA, X-linked,

or autosomal genes in resolving the rapid Felidae species radiation.
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Publisher Place of Publication Washington D.C. Editor Jill Pecon-Slattery, W.J.M., Agostinho Antunes, Emma Teeling, Stephen J.O'Brien
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rana @ 880 Serial (up) 1008
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Author Wasser, S.
Title Snow Leopard Genetics: New Techniques Type Miscellaneous
Year 1998 Publication Abbreviated Journal
Volume xvi Issue Pages 8
Keywords genetics; medicine; veterinary; Dna; feces; scat; browse; 4500
Abstract
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Publisher Islt Place of Publication Seattle Editor
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Full Text at URLJournal Title: Snow Line Approved no
Call Number SLN @ rana @ 438 Serial (up) 1009
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Author Wharton, D.; Freeman, H.
Title The Snow Leopard in North America: Captive Breeding Under the Species Survival PLan Type Conference Article
Year 1988 Publication Abbreviated Journal
Volume Issue Pages 131-136
Keywords Species-Survival-Plan; captivity; breeding; genetics; zoos; studs; captive; browse; species; survival; plan; 1720
Abstract
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Publisher International Snow Leoaprd Trust and WIldlife Institute of India Place of Publication India Editor H.Freeman
Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Full Text at URLTitle, Monographic: Fifth International Snow Leopard SymposiumPlace of Meeting: Srinagar, IndiaDate of Copyright: 1988 Approved no
Call Number SLN @ rana @ 134 Serial (up) 1016
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Author Wildt, D.; Pukazhenthi, B.; Brown, J.; Monfort, S.; Howard, J.; Roth, T.
Title Spermatology for understanding, managing and conserving rare species Type Journal Article
Year 1995 Publication Reproduction Fertility and Development Abbreviated Journal
Volume 7 Issue 4 Pages 811-824
Keywords Assisted-Reproduction; Cryopreservation; reproduction; zoo; medical; veterinary; spermatology; genetics; browse; assisted; 1380
Abstract Most conventional spermatology research involves common mammalian species including livestock, laboratory animals and humans. Yet, there are more than 4500 mammalian species inhabiting the planet for which little is known about basic reproductive biology, including sperm characteristics and function. This information is important, not just as adjunct knowledge, but because the majority of these species are threatened with extinction, largely due to human-induced pressures. The field of conservation is changing rapidly, and global cooperation is emerging among a variety of wildlife enthusiasts, ranging from management authorities of nature reserves to curators of rare zoological collections. Conservation progress depends on systematic, multidisciplinary research first to answer basic questions, with new data then applied to endangered species management plans. The reproductive physiologist is a crucial component of this scheme. Reproduction is the essence of species survival, and enormous effort needs to be directed at these 'untraditional' research species, subspecies and populations. Spermatology research combined with simultaneous efforts in endocrinology, embryology and cryopreservation (among others) can lead to the successful application of assisted reproduction. Examples from this laboratory include an array of wild felid species and a rare cervid and mustelid. Obstacles to success are formidable, including unique species-specificities, diminished genetic diversity and a general lack of resources. Nonetheless, the field offers tremendous opportunities for generating unique knowledge of comparative interest and with conservation utility.
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Language Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
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ISSN ISBN Medium
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Notes Document Type: English Approved no
Call Number SLN @ rana @ 278 Serial (up) 1024
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Author Zhang, F.; Jiang, Z.; Zeng, Y.; McCarthy, T.
Title Development of primers to characterize the mitochondrial control region of the snow leopard (Uncia uncia) Type Miscellaneous
Year 2007 Publication Molecular Ecology Notes Abbreviated Journal
Volume 7 Issue Pages 1196-1198
Keywords control region,Felidae,mitochondrial DNA,snow leopard,species-specific primers; genetics; development; mitochondrial; control; region; snow; snow leopard; snow-leopard; leopard; uncia
Abstract The snow leopard (Uncia uncia) is a rare carnivore living above the snow line in central Asia. Using universal primers for the mitochondrial genome control region hypervariable

region 1 (HVR1), we isolated a 411-bp fragment of HVR1 and then designed specific primers

near each end of this sequence in the conserved regions. These primers were shown to yield

good polymerase chain reaction products and to be species specific. Of the 12 snow leopards

studied, there were 11 segregating sites and six haplotypes. An identification case of snow

leopard carcass (confiscated by the police) proved the primers to be a useful tool for forensic

diagnosis in field and population genetics studies.
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Publisher Place of Publication Editor
Language Summary Language Original Title
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Notes Approved no
Call Number SLN @ rana @ 911 Serial (up) 1073
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Author Lovari, S., Boesi, R., Minder, I., Mucci, N., Randi, E., Dematteis, A., and Ale, S. B.
Title Restoring a keystone predator may endanger a prey species in a human-altered ecosystem: the return of the snow leopard to Sagarmatha National Park Type Journal Article
Year 2009 Publication Animal Conservation Abbreviated Journal
Volume 12 Issue Pages 559-570
Keywords conservation, food habits, genetics, Hemitragus jemlahicus, Himalayan tahr, management, microsatellite, predation, presence, scat, scat analysis, snow leopard, Uncia uncia
Abstract Twenty-five years ago, the snow leopard Uncia uncia, an endangered large cat, was eliminated from what is now Sagarmatha National Park (SNP). Heavy hunting pressure depleted that area of most medium-large mammals, before it became a park. After three decades of protection, the cessation of hunting and the recovery of wild ungulate populations, snow leopards have recently returned (four individuals). We have documented the effects of the return of the snow leopard on the population of its main wild prey, the Himalayan tahr Hemitragus jemlahicus, a 'near-threatened' caprin. Signs of snow leopard presence were recorded and scats were collected along a fixed trail (130 km) to assess the presence and food habits of the snow leopard in the Park, from 2004 to 2006. Himalayan tahr, the staple of the diet, had a relative occurrence of 48% in summer and 37% in autumn, compared with the next most frequent prey, musk deer Moschus chrysogaster (summer: 20%; autumn: 15%) and cattle (summer: 15%; autumn: 27%). In early summer, the birth rate of tahr (young-to-female ratio: 0.8-0.9) was high. The decrease of this ratio to 0.1-0.2 in autumn implied that summer predation concentrated on young tahr, eventually altering the population by removing the kid cohort. Small populations of wild Caprinae, for example the Himalayan tahr population in SNP, are sensitive to stochastic predation events and may be led to almost local extinction. If predation on livestock keeps growing, together with the decrease of Himalayan tahr, retaliatory killing of snow leopards by local people may be expected, and the snow leopard could again be at risk of local extinction. Restoration of biodiversity through the return of a large predator has to be monitored carefully, especially in areas affected by humans, where the lack of important environmental components, for example key prey species, may make the return of a predator a challenging event.
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Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
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ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rana @ Serial (up) 1122
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Author Manati, A.R.
Title Fur trade of large cats and the question of the subspecies status of leopards in Afghanistan (Der Handel mit Fellen von Grosskatzen und die Abklärung der Unterartenfrage beim Type Book Whole
Year 2008 Publication Abbreviated Journal
Volume Issue Pages 125
Keywords fur trade, genetics, leopard, Panthera pardus, snow leopard, subspecies, Uncia uncia
Abstract Over a time of four years the bazars of Afghanistan were surveyed for furs of spotted wild cats, in particular leopards and snow leopards. In 2004 in Kabul a total of 28 furs of leopards were purchased by shopkeepers and 21 sold at an average price of 825 $. In the same year 25 furs of snow leopards were purchased and 19 sold to clients at an average price of 583 $. In 2006 at a single inspection double as many furs of leopards were found to be offered for sale in comparison to the whole year of 2004. Also prices had increased over the two years by 20 % to an average of 1037 $. Similarly the number of furs of snow leopards at 21 pieces was higher than in 2004, and the prices had increased to an average of 652 $. In 2007 investigations rendered more difficult, because the authorities had started to control the fur trade, and the results are not unequivocal. Clients were without any exception foreigners.

Surveys in 2004 in Mazar-e-Sharif, Kunduz, Takhar and Faiz Abad, in 2006 additionally in Baharak and Iskashem in the province of Badakhshan, revealed a regular trade in furs of spotted cats, however not as extensive as in Kabul. The most interesting finding was a fur of a cheetah in Mazar-e-Sharif, the first record of this species after 35 years.

From the surveys can be concluded that leopards still exist in the whole range of its distribution area in Afghanistan. However they don't allow any conclusion on the population size and its threat by hunting. In contrast to the leopard there exists a recent estimation of the population size of the snow leopard, saying that there are still 100 to 200 snow leopards living in Afghanistan. On the basis of these figures as well as the numbers of furs traded annually a Population and Habitat Viability Analysis was conducted. The result of this analysis is alarming. It has to be assumed that the snow leopard will be extinct in Afghanistan within the next ten years. To improve the protection of spotted cats in Afghanistan it needs both, a better implementation of the existing legislation as well as an awareness campaign among potential clients, i. e. foreigners living in Afghanistan.

The second part of this thesis deals with the question of subspecies of leopards in Afghanistan. Out of the 27 subspecies described four are believed to exist in Afghanistan. However, according to a molecularbiological revision of the species there occurs only one subspecies in Afghanistan, Panthera pardus saxicolor. To clarify the subspecies question various measures of furs had been taken in the bazars. The results revealed that the leopards in Afghanistan are the biggest of its species. However a further differentiation according to the area of origin within the country was not possible. Also the traditional differentiation on the basis of colours and patterns on the furs was not possible.

In contrast to the molecularbiological investigations published not only samples of zoo animals were available in this study but also samples from the wild. The own results confim that almost all leopards from Afghanistan and Iran belong to one and the same subspecies, P. p. saxicolor. Only in the most eastern part of Afghanistan, the Indian leopard, Panthera pardus fusca, can be found. The International Studbook for the Persian Leopard was analysed. The whole population derives from a few founder animals, which were imported in the midth fifties from Iran and in the late sixties from Afghanistan. To avoid inbreeding later on the Iranian and the Afghan lines were mixed. A female imported in 1968 from Kabul to Cologne is represented in each of the more than 100 today living animals.Mixing the two lines subsequently is justified by the genetic results of this study. Recently acquired animals from the Caucasus, however, should be tested genetically before integrating them into the zoo population.
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Publisher University of Köln Place of Publication Germany Editor
Language German Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rana @ Serial (up) 1123
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Author McCarthy, T.; Murray, K.; Sharma, K.; Johansson, O.
Title Preliminary results of a long-term study of snow leopards in South Gobi, Mongolia Type Journal Article
Year 2010 Publication Cat News Abbreviated Journal
Volume Autumn Issue 53 Pages 15-19
Keywords snow leopard, Mongolia, monitor, population, Panthera, Snow Leopard Trust, Snow Leopard Conservation Fund, South Gobi, ecology, radio collar, GPS-satellite collar, home range, camera trapping, fecal genetics, occupancy modeling
Abstract Snow leopards Panthera uncia are under threat across their range and require urgent conservation actions based on sound science. However, their remote habitat and cryptic nature make them inherently difficult to study and past attempts have provided insufficient information upon which to base effective conservation. Further, there has been no statistically-reliable and cost-effective method available to monitor snow leopard populations, focus conservation effort on key populations, or assess conservation impacts. To address these multiple information needs, Panthera, Snow Leopard Trust, and Snow Leopard Conservation Fund, launched an ambitious long-term study in Mongolia’s South Gobi province in 2008. To date, 10 snow leo-pards have been fitted with GPS-satellite collars to provide information on basic snow leopard ecology. Using 2,443 locations we calculated MCP home ranges of 150 – 938 km2, with substantial overlap between individuals. Exploratory movements outside typical snow leopard habitat have been observed. Trials of camera trapping, fecal genetics, and occupancy modeling, have been completed. Each method ex-hibits promise, and limitations, as potential monitoring tools for this elusive species.
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Publisher Place of Publication Editor
Language English Summary Language Original Title
Series Editor Series Title Abbreviated Series Title
Series Volume Series Issue Edition
ISSN ISBN Medium
Area Expedition Conference
Notes Approved no
Call Number SLN @ rana @ Serial (up) 1151
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Author Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J.
Title Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples Type Journal Article
Year 2014 Publication Conservation Genetic Resource Abbreviated Journal
Volume 6 Issue 2 Pages 369:373
Keywords Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification
Abstract Molecular markers that can effectively identify noninvasively collected samples and provide genetic

information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many

noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the

domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard

populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and

population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers

facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.

endangered species
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Publisher Place of Publication Editor
Language Summary Language Original Title
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Notes Approved no
Call Number SLN @ rakhee @ Serial (up) 1427
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