Abstract: Conservation practices are supposed to get refined by advancing scientific knowledge. We study this phenomenon in the context of monitoring tiger populations in India, by evaluating the ‘pugmark census method’ employed by wildlife managers for three decades. We use an analytical framework of modern animal population sampling to test the efficacy of the pugmark censuses using scientific data on tigers and our field observations. We identify three critical goals for monitoring tiger populations, in order of increasing sophistication: (1) distribution mapping, (2) tracking relative abundance, (3) estimation of absolute abundance. We demonstrate that the present census-based paradigm does not work because it ignores the first two simpler goals, and targets, but fails to achieve, the most difficult third goal. We point out the utility and ready availability of alternative monitoring paradigms that deal with the central problems of spatial sampling and observability. We propose an alternative sampling-based approach that can be tailored to meet practical needs of tiger monitoring at different levels of refinement.

Abstract: This handbook provides an introduction to snow leopard population survey techniques, followed by a detailed account of camera trapping methods.During the 2002 through 2004 winter field seasons, the Snow Leopard Conservancy experimented with infrared camera trapping techniques to define a methodology suitable for the high altitude environment.
In 2001 and 2002, much of our time was spent familiarizing ourselves with various infrared camera traps, their operation and setup, and comparing the effectiveness of different models and sensor types. We placed infrared camera traps along frequently used travel corridors at or near scent-sprayed rocks (rock scents) and scrape sites within 16 km2 sampling cells between January and March in 2003 and 2004. A total of 66 and 49 captures of snow leopards were tallied during 2003 and 2004, resulting in an overall capture success of 8.91 and 5.63 individuals per 100 trap-nights, respectively. Capture probabilities ranged from 0.33 to 0.46. Density estimates ranged from 8.49 ± 0.22 individuals per 100 km2 in 2003 to 4.45 ± 0.16 in 2004, with the disparity between years largely attributed to different trapping densities. Snow leopard abundance estimates were calculated using the computer program CAPTURE.

Abstract: Knowledge of the basic reproductive physiology of snow leopards is required urgently in order to develop a suitable management conditions under captivity. In this study, the long-term monitoring of concentrations of three steroid hormones in fecal matter of three female snow leopards was performed using enzyme immunoassays: (1) estradiol-17&#946;, (2) progesterone and (3) cortisol metabolite. Two of the female animals were housed with a male during the winter breeding season, and copulated around the day the estradiol-17&#946; metabolite peaked subsequently becoming pregnant. The other female was treated in two different ways: (1) first housed with a male in all year round and then (2) in the winter season only. She did not mate with him on the first occasion, but did so latter around when estradiol-17&#946; metabolite peaked, and became pseudopregnant. During pregnancy, progesterone metabolite concentrations increased for 92 or 94 days, with this period being approximately twice as long as in the pseudopregnant case (31, 42, 49 and 53 days). The levels of cortisol metabolite in the pseudopregnant female (1.35 µg/g) were significantly higher than in the pregnant females (0.33 and 0.24 µg/g) (P<0.05). Similarly, during the breeding season, the levels of estradiol-17&#946; metabolite in the pseudopregnant female (2.18 µg/g) were significantly higher than those in the pregnant females (0.81 and 0.85 µg/g) (P<0.05). Unlike cortisol the average levels of estradiol-17&#946; during the breeding season were independent of reproductive success.
The hormone levels may also be related to housing conditions and the resulting reproductive success in female leopards. The female housed with a male during the non-breeding season had high levels of cortisol metabolites and low levels of estradiol-17&#946; in the breeding season, and failed to become pregnant. This indicates that housing conditions in snow leopards may be an important factor for normal endocrine secretion and resulting breeding success.

Abstract: Many mammalian herbivores show a temporal diet variation between graminoid-dominated and browse dominated diets. We determined the causes of such a diet shift and its implications for conservation of a medium sized ungulate-the bharal Pseudois nayaur. Past studies show that the bharal diet is dominated by graminoids (>80%) during summer, but the contribution of graminoids declines to about 50% in winter. We tested the predictions generated by two alternative hypotheses explaining the decline: low graminoid availability during winter causes bharal to include browse in their diet; bharal include browse, with relatively higher nutritional quality, in their diet to compensate for the poor quality of graminoids during winter. We measured winter graminoid availability in areas with no livestock grazing, areas with relatively moderate livestock grazing, and those with intense livestock grazing pressures. The chemical composition of plants contributing to the bharal diet was analysed. The bharal diet was quantiWed through signs of feeding on vegetation at feeding locations. Population structures of bharal populations were recorded using a total count method. Graminoid availability was highest in areas without livestock grazing, followed by areas with moderate and intense livestock grazing. The bharal diet was dominated by graminoids (73%) in areas with highest graminoid availability. Graminoid contribution to the bharal diet declined monotonically (50, 36%) with a decline in graminoid availability. Bharal young to female ratio was 3 times higher in areas with high graminoid availability than areas with low graminoid availability. The composition of the bharal winter diet was governed predominantly by the availability of graminoids in the rangelands. Our results suggest that bharal include more browse in their diet during winter due to competition from livestock for graminoids. Since livestock grazing reduces graminoid availability, creation of livestock-free areas is necessary for the conservation of grazing species such as the bharal and its predators including the endangered snow leopard in the Trans-Himalaya.

Abstract: This summary of a four year field study by Jackson and Ahlborn begging in 1982 and concluding in 1985, discusses behaviour, trapping and tracking techniques, home range, activity patterns, prey and habitat and survey methods.