Abstract: Under this grant awarded by The Leonard X. Bosack and Bette M. Kruger Foundation through the auspices of Cat Action Treasury, SLC set out to accomplish the following outcomes in our Stewardship Program:
As of July 1, 2003 we completed the following major activities:
* Tested and compared different remote sensor and camera configurations to determine which is most reliable at “capturing” passing snow leopards;
* Investigated sampling strategies and camera trap placement with respect to snow leopard travel patterns and marking behavior;
* Compared different survey methods: direct (non-invasive capture of photos and DNA material contained in hairs), and indirect (sign transects and presence/absence surveys under the standard SLIMS protocol);
* Assessed the attitude of local people toward snow leopards, wolves and other wildlife as well as their perceptions of benefits and costs associated with the Hemis National Park, in order to craft more effective conservation and park management measures.
These activities mesh with SLC’s ongoing program of predator-proofing livestock corrals in settlements of the Hemis National Park, as well as outside protected areas (including Zanskar, Lingshed and Kanji. For each village’s corrals that are improved, we estimate that five or more snow leopards are saved from retaliatory killing by shepherds who lose valuable livestock.
Our program in assisting villagers to gain supplementary income from tourism-related activities is gaining strength, with trainings in 10 settlements this spring. SLC brought staff of KCC, the Khangchenjunga Conservation Committee, a local NGO based in Sikkim to assist in the skills training and to exchange ideas and experiences from other areas.
Following on from the groundwork laid during the first Bosack-Kruger grant to SLC, we launched a major initiative in collaboration with the Mountain Institute, the Himalayan Homestay program. Funding for this is being provided by UNESCO.
For further information on these and other snow leopard conservation efforts, see our newly designed web-page.
The following paragraphs summarize our accomplishments to date, supported by this grant. For detailed information, please consult the following reports, which are being mailed under separate cover:
1) “Local People’s Attitudes toward Wildlife Conservation in Hemis National Park with Special Reference to the Conservation of Large Predators” (prepared by Rodney Jackson, Rinchen Wangchuk and Jigmet Dadul)
2) “Non-Invasive Monitoring to Support Stewardship of Snow Leopards and Their Prey:
Evaluation of Remote Camera Traps for Censusing Snow Leopards” (prepared by Rodney Jackson and Jerry Roe).

Abstract: Molecular markers that can effectively identify noninvasively collected samples and provide genetic
information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many
noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the
domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard
populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and
population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers
facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.

Abstract: The snow leopard (Panthera uncia) is an apex predator on the Tibetan Plateau and in the surrounding mountain ranges. It is listed as Vulnerable in the IUCN's Red List. The large home range and low population densities of this species mandate range-wide conservation prioritization. Two efforts for range-wide snow leopard conservation planning have been conducted based on expert opinion, but both were constrained by limited knowledge and the difficulty of evaluating complex processes, such as connectivity across large landscapes. Here, we compile > 6000 snow leopard occurrence records from across its range and corresponding environmental covariates to build a model of global snow leopard habitat suitability. Using spatial prioritization tools, we identi!ed seven large continuous habitat patches as global snow leopard Landscape Conservation Units (LCUs). Each LCU faces differing threat levels from poaching, anthropogenic development, and climate change. We identi!ed ten po- tential inter-LCU linkages, and centrality analysis indicated that Tianshan-Pamir-Hindu Kush-Karakorum, Altai, and the linkage between them play a critical role in maintaining the global snow leopard habitat connectivity.

Abstract: This study was undertaken in spring, 2019 to assess the applicability of the double-observer survey method for estimating blue sheep Pseudois nayaur abundance in Nar-Phu valley of Manang District located in Annapurna Conservation Area of northern Nepal. Since counting large mammals in rugged mountain habitat poses a special challenge, we tested the efficacy of the double observer method for generating robust population estimates for this important protected area. The overall detection probability for observers (O1 and O2) was 0.94 and 0.91 for a total of 106 groups comprised of 2059 individual blue sheep. We estimated the area’s blue sheep population at 2070 (SE ± 168.77; 95% CI 2059–2405) for the 246.2 km2 of sampled habitat. We determined blue sheep to be widely distributed within the study area with a mean density of 8.4 individuals per km2 based on a total study area of 246.2 km2. We discuss demographic population structure and identify limitations when applying the double observer approach, along with recommending viewshed mapping for ensuring more robust density estimates of mountain-dwelling ungulates like blue sheep or ibex that inhabit extremely heterogeneous terrain which strongly influences sighting distances and overall animal detection rates.

Abstract: Understanding landscape connectivity and population genetic parameters is imperative for threatened species management. However, such information is lacking for the snow leopard (Panthera uncia). This study sought to explore hierarchical snow leopard gene flow patterns and drivers of genetic structure in Mongolia and China. A total of 97 individuals from across Mongolia and from the north-eastern edge of the Qinghai-Tibetan Plateau in Gansu Province to the middle of Qinghai Province in China were genotyped across 24 microsatellite loci. Distance-based frameworks were used to determine a landscape scenario best explaining observed genetic structure. Spatial and non-spatial methods were used to investigate fine-scale autocorrelation and similarity patterns as well as genetic structure and admixture. A genetic macro-division between populations in China and Mongolia was observed, suggesting that the Gobi Desert is a substantial barrier to gene flow. However, admixture and support for a resistance-based mode of isolation suggests connective routes that could facilitate movement. Populations in Mongolia had greater connectivity, indicative of more continuous habitat. Drivers of genetic structure in China were difficult to discern, and fine-scale sampling is needed. This study elucidates snow leopard landscape connectivity and helps to prioritize conservation areas. Although contact zones may have existed and occasional crossings can occur, establishing corridors to connect these areas should not be a priority. Focus should be placed on maintaining the relatively high connectivity for snow leopard populations within Mongolia and increasing research efforts in China.

Abstract: In the Nepalese Himalaya, conflict with rural communities due to livestock predation to large carnivores like snow leopard, common leopard, wolf and wild dog has risen sharply in recent years. This increase is attributed to a number of factors, including implementation and enforcement of wildlife protection laws (which have permitted a recovery in carnivore numbers), the creation of protected areas (which serve as refuges from which predators can populate the surrounding area), the depletion of natural prey due to poaching and loss of habitat, and lax livestock herding practices. However, little information is presently available upon which to design remedial programs. U.S. AID provided research funding for an in-depth assessment of snow leopard predation in the Annapurna Conservation Area (ACAP), an new innovative approach to nature conservation. Baseline information on livestock numbers and mortality were gathered during household interviews, followed by field surveys to assess animal husbandry systems, map pastures, establish periods of use and estimate stocking rates, and to characterize habitat using randomly located plots. Data substantiate the existence of depredation “hotspots”, where high loss occurs, in some cases exceeding 14% to 20% of the livestock population over a short period. Losses varied seasonally, and from year to year. Small-bodied stock like goat and sheep were more vulnerable than large-bodied stock like yak, although horses were especially vulnerable. Factors most closely associated with predation included lack of guarding (or very lax supervision), especially during the daytime, and repeated use of pastures where livestock depredators were known to be actively hunting. Herders usually reacted to repeated depredation incidents by attempting to trap or shoot the suspected culprit until losses declined to an acceptable level. As large carnivore populations become increasingly fragmented and genetically isolated, new management strategies are urgently needed, especially within the buffer zones and intervening corridors between separated parks and reserves. People reside within nearly all Himalayan protected areas, and such issues as loss of livestock and competition between wildlife and livestock cannot be avoided. A plan is offered for alleviating livestock loss in the Annapurna Conservation Area that involves local institutions in decision-making, rewards sound husbandry practices, strengthens indigenous institutions, without further eroding ACAP’s unique biological diversity and diverse carnivore population. The authors believe these measures and ideas could be fruitfully extended to other parts of the Himalaya.