Abstract: Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species.

Abstract: Observed a total of 109 copulations in 6 male-female pairs from 4 species of large Felidae. The mean intromission durations were 3.0 sec for Asian leopards (Panthera pardus), 3.3 sec for African leopards (P. pardus), 12.9 sec for snow leopards (Uncia uncia), 2.3 sec for spotted jaguars (P. onca), 3.3 sec for black jaguars (P. onca), and 12.4 sec for Siberian tigers (P. tigris). Behavioral patterns were qualitatively similar across species; all displayed a copulatory pattern with no lock, no intravaginal thrusting, ejaculation on a single insertion, and multiple ejaculations. Whereas domestic cats are reported to assume a neck grip and to tread prior to insertion, these larger Felidae generally did so after intromission had been achieved. After copulation, females of some pairs swiped at the male and displayed a rolling after-reaction. (18 ref) (PsycINFO Database Record (c) 2000 APA, all rights reserved)(unassigned)

Abstract: Anecdotal account with some general research results of a four year tracking study of the snow leopard in Nepal's Langu valley

Abstract: Large carnivores are of vital importance to the stability and integrity of most ecosystems, but recent declines in free-ranging populations have highlighted the potentially devastating effect of infectious diseases on their conservation. We reviewed the literature on infectious diseases of 34 large (maximum body mass of adults >20 kg) terrestrial carnivore species, 18 of which are considered to be threatened in the wild, and examined reports of antibody prevalence (seroprevalence) and cases of infection, mortality and population decline. Of 52 diseases examined, 44% were viral, 31% bacterial and the remainder were protozoal or fungal. Many infections were endemic in carnivores and/or infected multiple taxonomic families, with the majority probably occurring via inhalation or ingestion. Most disease studies consisted of serological surveys for disease antibodies, and antibody detection tended to be widespread implying that exposure to micro-organisms was common. Seroprevalence was higher in tropical than temperate areas, and marginally higher for infections known to occur in multiple carnivore groups. Confirmation of active infection via micro-organism recovery was less common for ursids than other taxonomic groups. Published descriptions of disease-induced population decline or extinction were rare, and most outbreaks were allegedly the result of direct transmission of rabies or canine distemper virus (CDV) from abundant carnivore species to less-common large carnivores. We conclude that the threat of disease epidemics in large carnivores may be serious if otherwise lethal infections are endemic in reservoir hosts and transmitted horizontally among taxa. To prevent or mitigate future population declines, research efforts should be aimed at identifying both the diseases of potential importance to large carnivores and the ecological conditions associated with their spread and severity.

Abstract: The author offers advice on how a captive-raised snow leopard cub could be acclimated to humans so it could be used as a zoo “ambassador”. The cub had negative experiences with humans and lacked socialization with other animals and conspecifics. Methods of avoiding and redirecting the cub's aggressive behavior are suggested. lgh.

Abstract: relationships among extant felid taxa are controversial. A historical appraisal addresses component congruence among statements on felid phylogenetic relationships, and monophyly of generic ranks proposed for felids is discussed. Felid cranial morphology (especially the masticatory apparatus, basicranium, and rostral regions) is examined, and 44 characters are postulated for 39 taxa. Internal congruence for these characters is evaluated and 27 components are suggested. Parsimony analysis, using the successive weighting option of Hennig86, of the 44 cranial characters plus 13 other morphological features yields 29 components in a “modified Nelson” consensus cladogram. Two basal, well resolved clades are hypothesized in the total morphology analysis; under parenthetical notation the first is: (Hepailurus yagouaroundi (Puma concolor (Acinonyx jubatus (Uncia uncia (Neofelis nebulosa (Panthera tigris (P. onca, P. leo, and P. pardus)))))). The second clade is: Profelis temmincki (P. badia (Pardofelis marmorata ((Caracal caracal (Lynx rufus (L. lynx (L. pardina (L. canadensis)))) (Felis chaus (F. lybica (L. cafra (L. silvestris (F. bieti (F. nigripes (F. margarita (Octocolobus manul)))))))). Prionailurus planiceps and P. viverrina formed another group which is suggested as the basal branch of the felid phylogeny. The results in this study do not support monophyly of Leopardus Gray, 1841; Profelis Severtzon, 1858; and Prionailurus Severtzon, 1858. A better supported, more highly resolved, felid phylogenetic tree is needed.

Abstract: This paper deals with the geographical distribution of ounces (Panthera uncia) in Qinghai Province. Ounces are distributed in 20 counties- Guide, Huzhu, Menyuan, Qilian, Tianjun, Dulan, Golmud, Guinan, Xinghai, Zhidoi, Zadoi, Nangqen, Yushu, Chindu, Qumarleb, Madio, Maqen, Jigzhi, Baima, Darlag. Among them, there fore 4 counties- Qilian, Tianjun, Dulan, Zadoi, in which the number of ounces are bigger. The number of ounces are shown in table 2. There are altogether 73 ounces (40 male, 33 female) which is supported to every park of China for ornamental, they were captured by fellow-villagers, and 44 ounces (23 male, 21 female) of them are below 6 months old, 9 ounces (6 male, 3 female) of them are 1 year old, 2 ounces (male) are 2 years old, and 18 ounces (9 male, 9 female) are adults.
Ounces live at an altitude of 3000-4100 metres above the sea, and prefer to eat Bharal (Pseudois noyour). Its breeding period goes from April to June, the number of embryos being 2-3.
A female ounce was successfully reproduced for the first time at Xining People's Park of China, in Spetember, 1984, and she gave birth to 3 young ounces.

Abstract: The Snow leopard (Panthera uncia) is one of the most threatened wild big cats within its range of distribution, however, the question of its systematic status is a matter of debate. Is it a member of genus Panthera, or is it in its own genus (Uncia)? The analysis of genetic difference at the DNA level may provide useful data to clarify the issue. In the present study, ten hexanucleotide-specific restriction endonucleases were used to evaluate the patterns of mitochondrial DNA variation between the Snow leopard and leopard (P. pardus). The molecular size of mtDNA from the two species was about 16.5 kb. Ten enzymes surveyed 32-34 restriction sites, which corresponded to 192 apprx 204 base pairs, or 1.16% apprx 1.24% of the total mtDNA molecule. A total of 45 restriction sites were mapped; of these sites, twenty-four, which correspond to 53.3% of the total sites, were variable. The sequence divergence between them was 0.075 33, which was undoubtedly in the species-level distinction but did not reach the genus level. Therefore, the Snow leopard should be placed in the genus Panthera rather than in its own ganus. It also seems reasonable to recognize Uncia as a valid subgenus. This conclusion not only support but also supplement the viewpoint of Simpson who treated Uncia as a subgenus within Panthera.

Abstract: Snow leopards occur near the snow line in northern Pakistan in the districts of Swat, Dir and Chitral of the Northwest Frontier Province (NWFP), Muzaffarabad district in Azad Kashmir and Gilgit and Baltistan districts in the Northern Areas. Although a number of protected areas are present in the form of national parks, wildlife sanctuaries and game reserves (Table 1) where legal protection is available to all wildlife species, including snow leopards, the status of this endangered species is not improving satisfactorily. The reasons are many and range from direct persecution by livestock owners to the less than strict management of protected areas.
Because of remote and inaccessible locations and lack of proper communication with local communities, government officials and nongovernmental organizations (NGOs) concerned with conservation find it difficult to obtain statistics on mortality of snow leopards. However, the killing of snow leopards is not uncommon. Because of the close and long-term association between local villagers and snow leopards, it is only through the support and cooperation of these peoples that protection of this endangered species can be assured against most of the existing threats. The effects of such cooperation has been clearly shown through some of the conservation projects of World Wildlife Fund (WWF) – Pakistan. Details of such projects and certain lessons that can be learned from these and similar projects are discussed in this paper.