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Author | Janecka; J.E.; Jackson, R.; Yuquang, Z.; Diqiang, L.; Munkhtsog, B.; Buckley-Beason, V.; Murphy, W.J. | ||||
Title | Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study | Type | Miscellaneous | ||
Year | 2008 | Publication | Animal Conservation | Abbreviated Journal | |
Volume | 11 | Issue | Pages | 401-411 | |
Keywords | snow leopard; genetics; scat; noninvasive; survey. | ||||
Abstract | The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards. | ||||
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Supplemental information may be found at http:snowleopardnetwork.org/bibliography/JaneckaSupplemental2008.pdf | Approved | no | ||
Call Number | SLN @ rana @ 883 | Serial | 488 | ||
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Author | Anwar, M., Jackson, R., Nadeem, M., Janecka, J., Hussain, S., Beg, M., Muhammad, G., and Qayyum, M. | ||||
Title | Food habits of the snow leopard Panthera uncia (Schreber, 1775) in Baltistan, Northern Pakistan | Type | Journal Article | ||
Year | 2011 | Publication | European Journal of Wildlife Research | Abbreviated Journal | |
Volume | Issue | 3 March | Pages | 1-7 | |
Keywords | Himalayas, Karakoram, Scat, Diet, Hair, Livestock, Biomass | ||||
Abstract | The snow leopard (Panthera uncia) inhabits the high, remote mountains of Pakistan from where very little information is available on prey use of this species. Our study describes the food habits of the snow leopard in the Himalayas and Karakoram mountain ranges in Baltistan, Pakistan. Ninety-five putrid snow leopard scats were collected from four sites in Baltistan. Of these, 49 scats were genetically confirmed to have originated from snow leopards. The consumed prey was identified on the basis of morphological characteristics of hairs recovered from the scats. It was found that most of the biomass consumed (70%) was due to domestic livestock viz. sheep (23%), goat (16%), cattle (10%), yak (7%), and cattle–yak hybrids (14%). Only 30% of the biomass was due to wild species, namely Siberian ibex (21%), markhor (7%), and birds (2%). Heavy predation on domestic livestock appeared to be the likely cause of conflict with the local inhabitants. Conservation initiatives should focus on mitigating this conflict by minimizing livestock losses. | ||||
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Publisher | Springer Berlin / Heidelberg | Place of Publication | Editor | ||
Language | English | Summary Language | Original Title | ||
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ISSN | 1612-4642 | ISBN | Medium | ||
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Approved | no | |||
Call Number | SLN @ rana @ | Serial | 1304 | ||
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Author | Rosen, T. Hussain, S. Mohammad, G. Jackson, R. Janecka, J, E. Michel, S. | ||||
Title | Reconciling Sustainable Development of Mountain Communities With Large Carnivore Conservation | Type | Journal Article | ||
Year | 2012 | Publication | Mountain Research and Development | Abbreviated Journal | |
Volume | Issue | 32(3) | Pages | 286-293 | |
Keywords | Carnivores; conflict; conservation; incentives; livestock; insurance scheme; community empowerment; Pakistan | ||||
Abstract | While the world is becoming increasingly interconnected and interdependent, physically and culturally, the wildlife of remote mountain regions is being affected both positively and negatively by such interconnectedness. In the case of snow leopards, the conservation impact has been largely, and rather unexpectedly, positive: Species-focused conservation projects, such as Project Snow Leopard (PSL) in Gilgit-Baltistan, remain mainly externally driven initiatives. PSL, initiated as a small pilot project in 1998, has relied on an approach that includes the use of an insurance scheme, the deployment of mitigation measures, and the empowerment of local governance. This approach has been successful in reducing the conflict with snow leopards and has built greater tolerance toward them. PSL is managed by local communities and cofinanced by them. PSL communities throughout the region are bearing the burden of carnivore conservation, and they are unwittingly subsidizing their populations by ‘‘feeding’’ them their livestock even though they are an economic threat to them. In this article, we argue that external intervention in the form of efforts that help alleviate the consequences of conflict through local empowerment have had a positive impact on the local mountain societies. We also show that such interventions have resulted in tangible conservation results, with the number of snow leopards staying at least stable. Our experience also shows that while the incentive component is critical, it is also part of a larger approach—one that includes developing and supporting local governance structures, improving access to education, and offering a range of tools to reduce the conflict that can be implemented locally. Finally, we suggest that investing in this approach— one that recognizes the species and local-context complexities surrounding the implementation of conservation incentives—can continue to inform international practices and guidelines for reducing human–wildlife conflicts worldwide. |
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Approved | no | |||
Call Number | SLN @ rakhee @ | Serial | 1387 | ||
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Author | Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. | ||||
Title | Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples | Type | Journal Article | ||
Year | 2014 | Publication | Conservation Genetic Resource | Abbreviated Journal | |
Volume | 6 | Issue | 2 | Pages | 369:373 |
Keywords | Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification | ||||
Abstract | Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. endangered species |
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Approved | no | |||
Call Number | SLN @ rakhee @ | Serial | 1427 | ||
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Author | Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. | ||||
Title | Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples | Type | Journal Article | ||
Year | 2014 | Publication | Conservation Genetic Resource | Abbreviated Journal | |
Volume | 6 | Issue | 2 | Pages | 369:373 |
Keywords | Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification | ||||
Abstract | Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. |
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Approved | no | |||
Call Number | SLN @ rakhee @ | Serial | 1428 | ||
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Author | Janecka, J. E., Alves, P., Karmacharya, D., Samsel, N., Cheng, E., Tallmom, D., Schwartz, M. | ||||
Title | Wildlife Genetics in Mountainous Rugged Asian Landscapes: Methods, Applications and Examples | Type | Book Chapter | ||
Year | 2013 | Publication | Wildlife Research Techniques in rugged Mountainous Asian Landscapes | Abbreviated Journal | |
Volume | Issue | Pages | 44-91 | ||
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Approved | no | |||
Call Number | SLN @ rakhee @ | Serial | 1429 | ||
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Author | Rodgers, T. W.,Janecka, J. E. | ||||
Title | Applications and techniques for non-invasive faecal genetics research in felid conservation | Type | Journal Article | ||
Year | 2013 | Publication | Abbreviated Journal | ||
Volume | Issue | Pages | |||
Keywords | Conservation . Elusive species . Faecal DNA . Felidae . Non-invasive genetics | ||||
Abstract | Non-invasive genetic techniques utilising DNA extracted from faeces hold great promise for felid conservation research. These methods can be used to establish species distributions, model habitat requirements, analyse diet, estimate abundance and population density, and form the basis for population, landscape and conservation genetic analyses. Due to the elusive nature of most felid species, non-invasive genetic methods have the potential to provide valuable data that cannot be obtained with traditional observational or capture techniques. Thus, these methods are particularly valuable for research and conservation of endangered felid species. Here, we review recent studies that use non-invasive faecal genetic techniques to survey or study wild felids; provide an overview of field, laboratory and analysis techniques; and offer suggestions on how future non-invasive genetic studies can be expanded or improved to more effectively support conservation. |
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Approved | no | |||
Call Number | SLN @ rakhee @ | Serial | 1430 | ||
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Author | Hacker, C., Atzeni, L., Munkhtsog, B., Munkhtsog, B., Galsandorj, N., Zhang, Y., Liu, Y., Buyanaa, C., Bayandonoi, G., Ochirjav, M., Farrington, J. D., Jevit, M., Zhang, Y., Wu, L. Cong, W., Li, D., Gavette, C., Jackson, R., Janecka, J. E. | ||||
Title | Genetic diversity and spatial structures of snow leopards (Panthera uncia) reveal proxies of connectivity across Mongolia and northwestern China | Type | Journal Article | ||
Year | 2022 | Publication | Landscape Ecology | Abbreviated Journal | |
Volume | Issue | Pages | 1-19 | ||
Keywords | Admixture · Central Asia · Connectivity · Habitat Modeling · Landscape Genetics · Noninvasive Genetics · MEM · Spatial Structures | ||||
Abstract | Understanding landscape connectivity and population genetic parameters is imperative for threatened species management. However, such information is lacking for the snow leopard (Panthera uncia). This study sought to explore hierarchical snow leopard gene flow patterns and drivers of genetic structure in Mongolia and China. A total of 97 individuals from across Mongolia and from the north-eastern edge of the Qinghai-Tibetan Plateau in Gansu Province to the middle of Qinghai Province in China were genotyped across 24 microsatellite loci. Distance-based frameworks were used to determine a landscape scenario best explaining observed genetic structure. Spatial and non-spatial methods were used to investigate fine-scale autocorrelation and similarity patterns as well as genetic structure and admixture. A genetic macro-division between populations in China and Mongolia was observed, suggesting that the Gobi Desert is a substantial barrier to gene flow. However, admixture and support for a resistance-based mode of isolation suggests connective routes that could facilitate movement. Populations in Mongolia had greater connectivity, indicative of more continuous habitat. Drivers of genetic structure in China were difficult to discern, and fine-scale sampling is needed. This study elucidates snow leopard landscape connectivity and helps to prioritize conservation areas. Although contact zones may have existed and occasional crossings can occur, establishing corridors to connect these areas should not be a priority. Focus should be placed on maintaining the relatively high connectivity for snow leopard populations within Mongolia and increasing research efforts in China. | ||||
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Call Number | SLN @ rakhee @ | Serial | 1717 | ||
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Author | Robinson, J. J., Crichlow, A. D., Hacker, C. E., Munkhtsog, B., Munkhtsog, B., Zhang, Y., Swanson, W. F., Lyons, L. A., Janecka, J. E. | ||||
Title | Genetic Variation in the Pallas’s Cat (Otocolobus manul) in Zoo-Managed and Wild Populations | Type | Journal Article | ||
Year | 2024 | Publication | Diversity | Abbreviated Journal | |
Volume | 16 | Issue | 228 | Pages | 1-13 |
Keywords | Otocolobus manul; microsatellites; zoo-managed population; mitochondrial 12S ribosomal RNA; endothelial PAS domain protein 1 | ||||
Abstract | The Pallas’s cat (Otocolobus manul) is one of the most understudied taxa in the Felidae family. The species is currently assessed as being of “Least Concern” in the IUCN Red List, but this assessment is based on incomplete data. Additional ecological and genetic information is necessary for the long-term in situ and ex situ conservation of this species. We identified 29 microsatellite loci with sufficient diversity to enable studies into the individual identification, population structure, and phylogeography of Pallas’s cats. These microsatellites were genotyped on six wild Pallas’s cats from the Tibet Autonomous Region and Mongolia and ten cats from a United States zoo-managed population that originated in Russia and Mongolia. Additionally, we examined diversity in a 91 bp segment of the mitochondrial 12S ribosomal RNA (MT-RNR1) locus and a hypoxia-related gene, endothelial PAS domain protein 1 (EPAS1). Based on the microsatellite and MT-RNR1 loci, we established that the Pallas’s cat displays moderate genetic diversity. Intriguingly, we found that the Pallas’s cats had one unique nonsynonymous substitution in EPAS1 not present in snow leopards (Panthera uncia) or domestic cats (Felis catus). The analysis of the zoo-managed population indicated reduced genetic diversity compared to wild individuals. The genetic information from this study is a valuable resource for future research into and the conservation of the Pallas’s cat. | ||||
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Approved | no | |||
Call Number | SLN @ rakhee @ | Serial | 1749 | ||
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