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Author | Rodgers, T. W.,Janecka, J. E. | ||||
Title | Applications and techniques for non-invasive faecal genetics research in felid conservation | Type | Journal Article | ||
Year | 2013 | Publication | Abbreviated Journal | ||
Volume | Issue | Pages | |||
Keywords | Conservation . Elusive species . Faecal DNA . Felidae . Non-invasive genetics | ||||
Abstract | Non-invasive genetic techniques utilising DNA extracted from faeces hold great promise for felid conservation research. These methods can be used to establish species distributions, model habitat requirements, analyse diet, estimate abundance and population density, and form the basis for population, landscape and conservation genetic analyses. Due to the elusive nature of most felid species, non-invasive genetic methods have the potential to provide valuable data that cannot be obtained with traditional observational or capture techniques. Thus, these methods are particularly valuable for research and conservation of endangered felid species. Here, we review recent studies that use non-invasive faecal genetic techniques to survey or study wild felids; provide an overview of field, laboratory and analysis techniques; and offer suggestions on how future non-invasive genetic studies can be expanded or improved to more effectively support conservation. |
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Notes | Approved | no | |||
Call Number | SLN @ rakhee @ | Serial | 1430 | ||
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Author | Johnson, W.E.; Eizirik, E.; Pecon-Slattery, J.; Murphy, W.J.; Antunes, A.; Teeling, E.; O'Brien, S.J. | ||||
Title | The Late Miocene Radiation of Modern Felidae: A Genetic Assessment | Type | Miscellaneous | ||
Year | 2006 | Publication | Science | Abbreviated Journal | |
Volume | 311 | Issue | Pages | 74-77 | |
Keywords | carnivore; classification; divergence; Dna; Felidae; fossil; lineages; Miocene; mitochondrial; Molecular; phylogeny; radiation; taxonomic | ||||
Abstract | Modern felid species descend from relatively recent (G11 million years ago) divergence and speciation events that produced successful predatory carnivores worldwide but that have confounded taxonomic classifications. A highly resolved molecular phylogeny with divergence dates for all living cat species, derived from autosomal, X-linked, Y-linked, and mitochondrial gene segments (22,789 base pairs) and 16 fossil calibrations define eight principal lineages produced through at least 10 intercontinental migrations facilitated by sea-level fluctuations. A ghost lineage analysis indicates that available felid fossils underestimate (i.e., unrepresented basal branch length) first occurrence by an average of 76%, revealing a low representation of felid lineages in paleontological remains. The phylogenetic performance of distinct gene classes showed that Y-chromosome segments are appreciably more informative than mitochondrial DNA, X-linked, or autosomal genes in resolving the rapid Felidae species radiation. | ||||
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Notes | Approved | no | |||
Call Number | SLN @ rana @ 908 | Serial | 502 | ||
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Author | Zhang, F.; Jiang, Z.; Zeng, Y.; McCarthy, T. | ||||
Title | Development of primers to characterize the mitochondrial control region of the snow leopard (Uncia uncia) | Type | Miscellaneous | ||
Year | 2007 | Publication | Molecular Ecology Notes | Abbreviated Journal | |
Volume | 7 | Issue | Pages | 1196-1198 | |
Keywords | control region,Felidae,mitochondrial DNA,snow leopard,species-specific primers; genetics; development; mitochondrial; control; region; snow; snow leopard; snow-leopard; leopard; uncia | ||||
Abstract | The snow leopard (Uncia uncia) is a rare carnivore living above the snow line in central Asia. Using universal primers for the mitochondrial genome control region hypervariable region 1 (HVR1), we isolated a 411-bp fragment of HVR1 and then designed specific primers near each end of this sequence in the conserved regions. These primers were shown to yield good polymerase chain reaction products and to be species specific. Of the 12 snow leopards studied, there were 11 segregating sites and six haplotypes. An identification case of snow leopard carcass (confiscated by the police) proved the primers to be a useful tool for forensic diagnosis in field and population genetics studies. |
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Notes | Approved | no | |||
Call Number | SLN @ rana @ 911 | Serial | 1073 | ||
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