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Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. |
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Title |
Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
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Journal Article |
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Year |
2014 |
Publication |
Conservation Genetic Resource |
Abbreviated Journal |
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6 |
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2 |
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369:373 |
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Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification |
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Abstract |
Molecular markers that can effectively identify noninvasively collected samples and provide genetic
information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many
noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the
domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard
populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and
population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers
facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. |
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SLN @ rakhee @ |
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1428 |
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Author |
Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. |
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Title |
Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples |
Type |
Journal Article |
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Year |
2014 |
Publication |
Conservation Genetic Resource |
Abbreviated Journal |
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Volume |
6 |
Issue |
2 |
Pages |
369:373 |
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Keywords |
Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification |
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Abstract |
Molecular markers that can effectively identify noninvasively collected samples and provide genetic
information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many
noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the
domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard
populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and
population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers
facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.
endangered species |
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SLN @ rakhee @ |
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1427 |
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Author |
Darehshuri, B.F. |
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Title |
Threatened cats of Asia |
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Journal Article |
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Year |
1978 |
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Wildlife |
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20 |
Issue |
9 |
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396-400 |
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Keywords |
Asia, Acinonyx jubatus, Asiatic cheetah, cheetah, Siberian tiger, snow leopard |
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Abstract |
Man's hand is turned against the wild cats wherever they occur, often due to the value of their fur, but also because of the danger they sometimes pose to domestic stock and even human beings. All the larger Asian cats are threatened, and on this and the following pages we look at three of them – the Asiatic cheetah, the Siberian tiger, and the snow leopard. |
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SLN @ rana @ |
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1116 |
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Author |
Chapron, G. |
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Title |
Re-wilding: other projects help carnivores stay wild |
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Journal Article |
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2005 |
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Nature |
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437 |
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318 |
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Keywords |
Acinonyx jubatus, carnivore, coexistence, conservation, damage prevention, Panthera leo, snow leopard, survival, Uncia uncia |
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Abstract |
Letter to Nature Editor, in response to: In their plea for bringing Pleistocene wildlife to the New World (“Re-wilding North America” Nature 436, 913–914; 2005), Josh Donlan and colleagues do not discuss successful efforts to ensure long-term survival of large carnivores in Africa and Asia. A few examples are given. |
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SLN @ rana @ |
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1114 |
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Author |
Ale, S.; Whelan, C. |
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Title |
Reappraisal of the role of big, fierce predators |
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Miscellaneous |
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2008 |
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Biodiversity Conservation |
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685-690 |
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Biodiversity ú Conservation ú Costs of predation ú Indirect effects ú Non-lethal effects ú Predators ú Top-down control; big; predators; predator |
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Abstract |
The suggestion in the early 20th century that top predators were a necessary component of ecosystems because they hold herbivore populations in check and promote biodiversity was at Wrst accepted and then largely rejected. With the advent of Evolutionary Ecology and a more full appreciation of direct and indirect effects of top predators, this role of top predators is again gaining acceptance. The previous views were predicated upon lethal effects of predators but largely overlooked their non-lethal effects. We suggest that
conceptual advances coupled with an increased use of experiments have convincingly demonstrated that prey experience costs that transcend the obvious cost of death. Prey species use adaptive behaviours to avoid predators, and these behaviours are not cost-free. With predation risk, prey species greatly restrict their use of available habitats and consumption of available food resources. Effects of top predators consequently cascade down to the trophic levels below them. Top predators, the biggies, are thus both the targets of and the means for conservation at the landscape scale. |
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English |
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SLN @ rana @ 885 |
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52 |
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Author |
Meiers, S.T. |
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Title |
Habitat use by captive puma (Felis concolor) and snow leopards (Pathera uncia) at the Lincoln Park Zoo, Chicago, Illinois |
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Book Whole |
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1992 |
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1-74 |
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habitat; habitat use; use; captive; felis; Felis-concolor; concolor; snow; snow leopards; snow leopard; snow-leopards; snow-leopard; leopards; leopard; uncia; park; zoo; 1990; observations; panthera; panthera uncia; Panthera-uncia; zoological; zoological gardens; zoological-gardens; gardens; behavior; species; Time budget; dens; Identity; Animals; Animal; non; Cats; cat; location; relief |
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Between May 1990 and January 1991, behavioral observations were made of two captive pumas (Felis concolor Linnaeus), and two captive snow leopards (Panthera uncia Schreber) in their outdoor exhibits at the Lincoln Park Zoological Gardens, Chicago, Illinois. Behaviors compared within and between species included: 1) time spend in the different habitat types; 2) time budgets for the different behaviors: laying, moving, sitting, standing, crouching, in the tree, drinking, urinating, defecating, within their inside dens, and “behavior not determined” when the identity or behavior of the individuals could not be determined; and 3) mobility of the animals within their exhibits. Also examined were: 4) preferences for different habitat types; 5) recommendations for future exhibit designs. Both species located themselves within their exhibits in a non-random manner. The majority of cats' time was spent in elevated locations (i.e., gunite ledges approximately 1-5.5 m above ground-level). Snow leopards exhibited this tendency to a greater extent than did the pumas. Both species also spent the majority of their time in the lying-down behavior; again snow leopards displayed this tendency significantly more than the pumas. Pumas were highly mobile and changed locations and behaviors in their exhibit significantly more than the snow leopards. No significant differences were noted between conspecifics in regard to habitat type preference, or mobility within the exhibit. Suggestions for future exhibit design include elevated locations for the cats to lay and look around within and outside their exhibits, caves for access to shade or relief from inclement weather, and ground surfaces to move about on. Features for exhibit design should take into consideration the natural habitat of the cat to occupy the exhibit. |
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Ph.D. thesis |
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DePaul University |
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A thesis submitted to the faculty of the Department of Biological Sciences in partial fulfillment of the degree requirements for the degree of Master of Science at DePaul University. |
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Call Number |
SLN @ rana @ 1008 |
Serial |
673 |
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Author |
Jackson, R.; Roe, J. |
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Title |
Preliminary Observations On Non-Invasive Techniques for Identifying Individual Snow Leopards and Monitoring Populations |
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Conference Article |
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2002 |
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snow; leopard; monitoring; population; uncia; non; invasive; photo; camera; trap; traps; 4970 |
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Islt |
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Abastract OnlyTitle, Monographic: Proceedings of the Snow Leopard Survival SummitPlace of Meeting: Seattle,WA |
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no |
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Call Number |
SLN @ rana @ 483 |
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469 |
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Author |
Macdonald, A.A.; Johnstone, M. |
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Title |
Comparative anatomy of the cardiac foramen ovale in cats (Felidae), dogs (Canidae), bears (Ursidae) and hyaenas (Hyaenidae) |
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Journal Article |
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1995 |
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J Anat |
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186 ( Pt 2) |
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235-243 |
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Animal; Newborn; Bears: anatomy & histology; Carnivora: anatomy & histology: embryology; Cats: anatomy & histology; Dogs: anatomy & histology; Heart Septum: anatomy & histology: embryology; Support,Non-U.S.Gov't; bear; anatomy; histology; Carnivora; embryology; Cats; cat; bears; Dog; Dogs; heart; septum; browse; non; gov't; us; government; 160 |
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The structure of the foramen ovale from 16 species representing 4 carnivore families, the Felidae, Canidae, Ursidae and Hyaenidae, was studied using the scanning electron microscope. The Felidae were represented by 9 domestic cat fetuses (Felis catus), 2 snow leopard neonates (Uncia uncia), an ocelot neonate (Leopardus pardalis), 2 lion neonates (Panthera leo), a panther neonate (Panthera pardus) and 3 tigers (Neofelis tigris), comprising 2 fetuses and a neonate. The Canidae were represented by a golden jackal neonate (Canis aureus), a newborn wolf (Canis lupus), 8 domestic dog fetuses (Canis familiaris), 3 red fox neonates (Vulpes vulpes) and a dhole neonate (Cuon alpinus). The Ursidae were represented by a brown bear neonate (Ursus arctos), a day-old grizzly bear cub (Ursus arctos horribilis), a polar bear neonate (Ursus maritimus), and 2 additional bear fetuses (species unknown). The Hyaenidae were represented by a striped hyaena neonate (Hyaena hyaena). In each species, the foramen ovale, when viewed from the terminal part of the caudal vena cava, had the appearance of a short tunnel. A thin fold of tissue, the developed remains of the embryonic septum primum, extended from the distal end of the caudal vena cava for a variable distance into the lumen of the left atrium and contributed towards the 'tunnel' appearance in all specimens. It constituted a large proportion of the tube, and its distal end was straight-edged. There was fibrous material underlying the endothelium of the flap, the apparent morphology of which suggested that it comprised cardiac muscle.(ABSTRACT TRUNCATED AT 250 WORDS) |
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0021-8782 |
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Document Type: eng |
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SLN @ rana @ 269 |
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632 |
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Author |
Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. |
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Title |
Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces |
Type |
Journal Article |
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Year |
1994 |
Publication |
Biol Reprod |
Abbreviated Journal |
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Volume |
51 |
Issue |
4 |
Pages |
776-786 |
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Keywords |
Animal; Carbon; Radioisotopes; Carnivora; Cats; Chromatography; High; Pressure; Liquid; Comparative Study; Estradiol; metabolism; Estrone; feces; chemistry; Female; Ovary; physiology; Pregnancy; Progesterone; Pseudopregnancy; Support; Non-U.S.Gov't; browse; non; government; gov't; us; 170 |
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Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species. |
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0006-3363 |
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Document Type: eng |
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SLN @ rana @ 251 |
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198 |
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De Groot, H.; Van Swieten, P.; Aalberse, R.C. |
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Title |
Evidence for a Fel d I-like molecule in the “big cats” (Felidae species) |
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Journal Article |
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Year |
1990 |
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J Allergy Clin Immunol |
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86 |
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1 |
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107-116 |
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Adolescence; Adult; Allergens; immunology; Animal; Antibodies; Monoclonal; diagnostic; use; Antibody; Specificity; Carnivora; Cats; Comparative; study; Cross; Reactions; Hair; Histamine; Release; Human; IgE; analysis; IgG; Middle; Age; Radioallergosorbent; Test; methods; Support; Non-U.S.Gov't; browse; us; government; gov't; 240 |
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Abstract |
In this study, we investigated the cross-reactivity pattern of IgE and IgG4 antibodies to the major feline allergen, Fel d I. We studied the IgE and IgG4 response of 11 cat-allergic patients against Fel d I-like structures in eight members of the Felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. Hair from these “big cats” was collected, extracted, and used in a RAST system and histamine-release test. By means of a RAST-inhibition assay with affinity-purified Fel d I from cat dander, it was established that, in the Felidae species, a Fel d I equivalent is present that reacts with IgE and IgG4 antibodies. We found that all patients had cross-reacting IgE antibodies to seven of the Felidae tested; no IgE antibodies reactive with the caracal were found. Eight of 10 patients with IgG4 antibodies directed to cat dander also had IgG4 antibodies directed to several Felidae species, including the caracal. However, the correlation between the IgE and the IgG4 antibody specificity was low, indicating that, in the case of Fel d I IgE and IgG4, antibodies do not necessarily have the same specificity. |
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0091-6749 |
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Document Type: eng |
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SLN @ rana @ 157 |
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233 |
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