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Author |
Schmidt, A.M.; Hess, D.L.; Schmidt, M.J.; Lewis, C.R. |
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Title |
Serum concentrations of oestradiol and progesterone and frequency of sexual behaviour during the normal oestrous cycle in the snow leopard (Panthera uncia) |
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Journal Article |
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Year |
1993 |
Publication |
J Reprod Fertil |
Abbreviated Journal |
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Volume |
98 |
Issue ![sorted by Issue field, ascending order (up)](img/sort_asc.gif) |
1 |
Pages |
91-95 |
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Keywords |
Animal; Animals; zoo; physiology; Carnivora; Estradiol; Blood; estrus; Female; Progesterone; Seasons; Sex; behavior; Support; U.S.Gov't; P.H.S.; browse; us; government; gov't; 490 |
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Abstract |
Serum oestradiol and progesterone concentrations were measured at weekly intervals for six months, and correlated with daily behavioural observations in two adult female snow leopards (Panthera uncia). Three oestradiol peaks (> 21 pg ml-1; interval 3.6 weeks) were identified in a snow leopardess housed alone (two more were probably missed because of the weekly sampling schedule), and three oestradiol peaks were identified in a snow leopardess housed with a male as a breeding pair (interval 6 weeks). Daily frequencies of feline reproductive behaviour averaged 1.77 observations per observation period during weeks of high oestradiol and 0.62 during weeks of low oestradiol. Progesterone concentrations did not rise above baseline values (< 2 ng ml-1) in the isolated animal, but 6 weeks of high progesterone concentrations (4.9- 38.8 ng ml-1) was recorded in the paired snow leopardess following mating. No offspring were produced. Snow leopards were observed daily for an additional 4.5 years. Sexual behaviour peaks could be clearly identified from December through April, and average daily sexual behaviour scores were higher during these months than during the rest of the year. Intervals between sexual behaviour peaks for the isolated snow leopardess averaged 3.03 weeks. The sexual behaviour of the paired snow leopards decreased for 8-9 weeks following mating when no offspring were produced, and decreased for 13 weeks in one year when a single cub was born. |
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0022-4251 |
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Document Type: eng |
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SLN @ rana @ 204 |
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874 |
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Trepanier, L.A.; Cribb, A.E.; Spielberg, S.P.; Ray, K. |
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Title |
Deficiency of cytosolic arylamine N-acetylation in the domestic cat and wild felids caused by the presence of a single NAT1-like gene |
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Journal Article |
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Year |
1998 |
Publication |
Pharmacogenetics |
Abbreviated Journal |
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Volume |
8 |
Issue ![sorted by Issue field, ascending order (up)](img/sort_asc.gif) |
2 |
Pages |
169-179 |
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Keywords |
Acetylation; Amino; Acid; Sequence; Animal; Arylamine; N-Acetyltransferase; metabolism; Base; Blotting; Southern; Carnivora; genetics; Cats; Cytosol; enzymology; Dna; Human; Isoenzymes; Liver; Molecular; Data; Polymerase; Chain; Reaction; Rabbits; Homology; Nucleic Acid; Substrate; Specificity; Support; U.S.Gov't; P.H.S.; browse; nucleic; us; government; 130 |
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The purpose of this study was to determine the molecular basis for a relative deficiency in the cat of cytosolic arylamine N- acetyltransferase (NAT), an enzyme family that is important in the metabolism of xenobiotics and that normally consists of at least two related enzymes, NAT1 and NAT2. N-acetyltransferase in feline liver showed high affinity (mean Km = 2.1 microM) for p-aminobenzoic acid, an NAT1 selective substrate in humans and rabbits, but showed a very poor affinity (mean Km > 10 mM) for sulfamethazine, an NAT2 selective substrate in humans and rabbits. Immunoreactive N-acetyltransferase was detected in feline liver, bladder and colon using an NAT1-specific antipeptide antibody, but was not detected in any tissues using an NAT2- specific antibody. Southern blot analysis of genomic DNA demonstrated a single band in domestic cats using each of six restriction digests; single bands were also found on Southern blot analysis of six wild felids. The deduced amino acid sequence of the central portion of feline N-acetyltransferase, obtained by polymerase chain reaction amplification in both domestic cats and seven wild felids (lion, tiger, lynx, snow leopard, bobcat, Asian leopard cat and cheetah), contained three residues, Phe125, Arg127, and Tyr129, which determine NAT1-like substrate specificity in humans. These results support the conclusion that cytosolic arylamine N-acetylation activity is low in the cat because of the presence of a single N-acetyltransferase that has substrate specificity, immunogenicity and sequence characteristics similar to human NAT1, and that the unusual presence of only a single N- acetyltransferase gene appears to be a family wide trait shared by other felids. |
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0960-314x |
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Document Type: eng |
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Call Number |
SLN @ rana @ 345 |
Serial |
968 |
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Roth, T.L.; Howard, J.G.; Donoghue, A.M.; Swanson, W.F.; Wildt, D.E. |
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Title |
Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro |
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Journal Article |
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Year |
1994 |
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J Reprod Fertil |
Abbreviated Journal |
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101 |
Issue ![sorted by Issue field, ascending order (up)](img/sort_asc.gif) |
3 |
Pages |
563-569 |
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Animal; Carnivora; physiology; Cell; survival; Cells; Cultured; Comparative; study; Culture; Media; Female; Male; Pentoxifylline; Sperm; Motility; Ovum; Interactions; Spermatozoa; cytology; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 190 |
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Electroejaculates from eight snow leopards were used to determine how the motility of spermatozoa was influenced by (i) type of media (Ham's F10, PBS, human tubal fluid or RPMI-1640); (ii) holding temperature (23 degrees C versus 37 degrees C); (iii) washing of spermatozoa and (iv) a sperm metabolic enhancer, pentoxifylline. The duration of sperm motility was assessed by evaluating samples in each treatment every hour for 6 h and a sperm motility index (a value combining percentage sperm motility and rate of forward progression) calculated. Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. During the 6 h co-incubation, the sperm motility index in PBS with pentoxifylline was greater (P < 0.05) than in PBS alone which, in turn, was greater (P < 0.05) than in the other three test media. Washing the spermatozoa enhanced (P < 0.05) motility in both PBS and PBS plus pentoxifylline relative to unwashed samples, but there was no effect (P > 0.05) of holding temperature. Pentoxifylline supplementation enhanced (P < 0.05) the proportion of cat eggs with bound, but not penetrated, snow leopard spermatozoa in the inner layer of the zona pellucida, and there were no spermatozoa in the perivitelline space.(ABSTRACT TRUNCATED AT 250 WORDS) |
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0022-4251 |
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Document Type: eng |
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Call Number |
SLN @ rana @ 253 |
Serial |
828 |
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Author |
Roth, T.L.; Armstrong, D.L.; Barrie, M.T.; Wildt, D.E. |
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Title |
Seasonal effects on ovarian responsiveness to exogenous gonadotrophins and successful artificial insemination in the snow leopard (Uncia uncia) |
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Journal Article |
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Year |
1997 |
Publication |
Reprod Fertil Dev |
Abbreviated Journal |
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Volume |
9 |
Issue ![sorted by Issue field, ascending order (up)](img/sort_asc.gif) |
3 |
Pages |
285-295 |
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Keywords |
Animal; Carnivora; anatomy; histology; Blood; physiology; Estradiol; Female; Gonadotropins; administration; dosage; pharmacology; Chorionic; Equine; Human; Insemination; artificial; methods; veterinary; Laparoscopy; Male; Oocytes; cytology; Ovary; drug; effects; Ovulation; Induction; Pregnancy; Progesterone; Seasons; Support; Non-U.S.Gov't; browse; non; us; gov't; government; 400 |
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Ovaries of the seasonally-breeding snow leopard (Uncia uncia) were examined to determine whether they were responsive to exogenous gonadotrophins throughout the year. The potential of laparoscopic artificial insemination (AI) also was assessed for producing offspring. During the non-breeding, pre-breeding, breeding and post-breeding seasons, females (n = 20) were treated with a standardized, dual- hormone regimen given intramuscularly (600 I.U. of equine chorionic gonadotrophin followed 80-84 h later with 300 I.U. of human chorionic gonadotrophin (hCG)). Laparoscopy was performed 45-50 h after administration of hCG, and all ovarian structures were described. Females with fresh corpora lutea (CL) were inseminated, and anovulatory females were subjected to follicular aspiration to examine oocyte quality. Snow leopards responded to exogenous gonadotrophins throughout the year. Mean number of total ovarian structures (distinct follicles mature in appearance plus CL) did not differ (P > or = 0.05) with season, but the proportion of CL: total ovarian structures was greater (P < 0.01) for the breeding season compared with all other seasons. The proportion of females ovulating was greater (P < 0.05) during the breeding and post-breeding seasons than during the pre-breeding and non- breeding seasons respectively. No Grade-1 quality oocytes were recovered from follicles of anovulatory females. Serum concentrations of oestradiol-17 beta appeared elevated in all females, and neither oestradiol-17 beta concentrations nor progesterone concentrations differed (P > or = 0.05) among seasons. Of 15 females artificially inseminated, the only one that was inseminated in the non-breeding season became pregnant and delivered a single cub. This is the first successful pregnancy resulting from AI in this endangered species. |
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1031-3613 |
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Document Type: eng |
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Call Number |
SLN @ rana @ 325 |
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830 |
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Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. |
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Title |
Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces |
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Journal Article |
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Year |
1994 |
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Biol Reprod |
Abbreviated Journal |
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51 |
Issue ![sorted by Issue field, ascending order (up)](img/sort_asc.gif) |
4 |
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776-786 |
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Animal; Carbon; Radioisotopes; Carnivora; Cats; Chromatography; High; Pressure; Liquid; Comparative Study; Estradiol; metabolism; Estrone; feces; chemistry; Female; Ovary; physiology; Pregnancy; Progesterone; Pseudopregnancy; Support; Non-U.S.Gov't; browse; non; government; gov't; us; 170 |
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Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species. |
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0006-3363 |
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Document Type: eng |
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SLN @ rana @ 251 |
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198 |
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Johnston, L.A.; Donoghue, A.M.; O'Brien, S.J.; Wildt, D.E. |
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Title |
Rescue and maturation in vitro of follicular oocytes collected from nondomestic felid species |
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Journal Article |
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Year |
1991 |
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Biol Reprod |
Abbreviated Journal |
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45 |
Issue ![sorted by Issue field, ascending order (up)](img/sort_asc.gif) |
6 |
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898-906 |
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Animal; Carnivora; anatomy; histology; physiology; Cells; Cultured; Female; Fertilization; Vitro; inVitro; Male; Oocytes; cytology; growth; development; ovarian; Follicle; Spermatozoa; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 230 |
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The potential for rescuing immature oocytes from the ovaries of females of rare felid species which die or undergo medical ovariohysterectomy was evaluated. Ovaries were recovered from 13 species representing 35 individuals in good-to-poor health. Although the majority of females were 10 yr of age or older and in fair-to-poor health, a total of 846 oocytes were recovered of which 608 (71.9%) were classified as fair-to- excellent quality. One hundred of these oocytes were used for initial maturation classification and as parthogenetic controls. Overall, of the 508 fair-to-excellent quality oocytes placed in culture, 164 (32.3%) matured to metaphase II in vitro. For species in which 3 or more individuals yielded oocytes, mean oocyte maturation rates were as follows: 36.2%, tiger; 27.9% leopard; and 8.3%, cheetah. In vitro insemination of oocytes resulted in fertilization (2 polar bodies, 2 pronuclei, or cleavage) rates of 9.1% to 28.6% (leopard) using homologous fresh spermatozoa and 4.0% (lion) to 40.0% (puma) using homologous frozen-thawed spermatozoa. Inseminations using heterologous (domestic cat) spermatozoa also resulted in fertilized oocytes in the tiger, leopard, snow leopard, puma, serval, and Geoffroy's cat (range in fertilization rate, 5.0% for leopard to 46.2% for puma). Cleaved embryos resulted from the insemination of leopard oocytes with homologous sperm (n = 1 embryo) and puma oocytes with domestic cat sperm (n = 3 embryos). These results demonstrate that immature ovarian oocytes from rare felid species can be stimulated to mature in vitro despite an excision-to-culture interval as long as 36 h.(ABSTRACT TRUNCATED AT 250 WORDS) |
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0006-3363 |
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Document Type: eng |
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SLN @ rana @ 172 |
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503 |
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