Abstract: Ovaries of the seasonally-breeding snow leopard (Uncia uncia) were examined to determine whether they were responsive to exogenous gonadotrophins throughout the year. The potential of laparoscopic artificial insemination (AI) also was assessed for producing offspring. During the non-breeding, pre-breeding, breeding and post-breeding seasons, females (n = 20) were treated with a standardized, dual- hormone regimen given intramuscularly (600 I.U. of equine chorionic gonadotrophin followed 80-84 h later with 300 I.U. of human chorionic gonadotrophin (hCG)). Laparoscopy was performed 45-50 h after administration of hCG, and all ovarian structures were described. Females with fresh corpora lutea (CL) were inseminated, and anovulatory females were subjected to follicular aspiration to examine oocyte quality. Snow leopards responded to exogenous gonadotrophins throughout the year. Mean number of total ovarian structures (distinct follicles mature in appearance plus CL) did not differ (P > or = 0.05) with season, but the proportion of CL: total ovarian structures was greater (P < 0.01) for the breeding season compared with all other seasons. The proportion of females ovulating was greater (P < 0.05) during the breeding and post-breeding seasons than during the pre-breeding and non- breeding seasons respectively. No Grade-1 quality oocytes were recovered from follicles of anovulatory females. Serum concentrations of oestradiol-17 beta appeared elevated in all females, and neither oestradiol-17 beta concentrations nor progesterone concentrations differed (P > or = 0.05) among seasons. Of 15 females artificially inseminated, the only one that was inseminated in the non-breeding season became pregnant and delivered a single cub. This is the first successful pregnancy resulting from AI in this endangered species.

Abstract: In this study, we investigated the cross-reactivity pattern of IgE and IgG4 antibodies to the major feline allergen, Fel d I. We studied the IgE and IgG4 response of 11 cat-allergic patients against Fel d I-like structures in eight members of the Felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. Hair from these “big cats” was collected, extracted, and used in a RAST system and histamine-release test. By means of a RAST-inhibition assay with affinity-purified Fel d I from cat dander, it was established that, in the Felidae species, a Fel d I equivalent is present that reacts with IgE and IgG4 antibodies. We found that all patients had cross-reacting IgE antibodies to seven of the Felidae tested; no IgE antibodies reactive with the caracal were found. Eight of 10 patients with IgG4 antibodies directed to cat dander also had IgG4 antibodies directed to several Felidae species, including the caracal. However, the correlation between the IgE and the IgG4 antibody specificity was low, indicating that, in the case of Fel d I IgE and IgG4, antibodies do not necessarily have the same specificity.

Abstract: Among large predators, snow leopards (Panthera uncia) and co-predators (e.g., wolves
Canis lupus, lynx Lynx lynx) often cause economic losses, engendering animosity from
local communities in the mountain ecosystem across south and central Asia (Din et al.,
2017; Jackson & Lama, 2016; Maheshwari, Takpa, Kujur, & Shawl, 2010; Schaller, 2012).
These economic losses range from around US $50 to nearly $300 per household,
a significant sum given per capita annual incomes of $250 – $400 (Jackson & Wangchuk,
2004; Mishra, 1997). Recent efforts such as improved livestock husbandry practices
(predator-proof livestock corrals – closed night shelters with covered roof with wiremesh
and a closely fitting iron or wooden door that can be securely locked at night) and
community-based ecotourism (e.g., home stays, guides, porters, pack animals, campsites)
are providing alternative livelihood opportunities and mitigating large carnivores – human
conflict in the snow leopard habitats (Hanson, Schutgens, & Baral, 2018; Jackson, 2015;
Jackson & Lama, 2016; Vannelli, Hampton, Namgail, & Black, 2019). Snow leopard-based
ecotourism provides an opportunity to secure livelihoods and reduce poverty of the
communities living in ecotourism sites across Ladakh (Chandola, 2012; Jackson, 2015).
To understand the role of snow leopard-based ecotourism in uplifting the financial profile
of local communities, mitigating large carnivore – human conflict and eventually changing
attitudes towards large carnivores in Hemis National Park, Ladakh, India, we compared
the estimated financial gains of a snow leopard-based ecotourism to stated livestock
predation losses by snow leopards and wolves.

Abstract: The threat posed by large carnivores to livestock and humans makes peaceful coexistence between
them difficult. Effective implementation of conservation laws and policies depends on the attitudes of local
residents toward the target species. There are many known correlates of human attitudes toward carnivores,
but they have only been assessed at the scale of the individual. Because human societies are organized hierarchically, attitudes are presumably influenced by different factors at different scales of social organization, but this scale dependence has not been examined.We used structured interview surveys to quantitatively assess the attitudes of a Buddhist pastoral community toward snow leopards (Panthera uncia) and wolves (Canis lupus).
We interviewed 381 individuals from 24 villages within 6 study sites across the high-elevation Spiti Valley in
the Indian Trans-Himalaya. We gathered information on key explanatory variables that together captured
variation in individual and village-level socioeconomic factors.We used hierarchical linear models to examine how the effect of these factors on human attitudes changed with the scale of analysis from the individual to the community. Factors significant at the individual level were gender, education, and age of the respondent (for wolves and snow leopards), number of income sources in the family (wolves), agricultural production, and large-bodied livestock holdings (snow leopards). At the community level, the significant factors included the number of smaller-bodied herded livestock killed by wolves and mean agricultural production (wolves) and village size and large livestock holdings (snow leopards). Our results show that scaling up from the individual to higher levels of social organization can highlight important factors that influence attitudes of people toward wildlife and toward formal conservation efforts in general. Such scale-specific information can help managers apply conservation measures at appropriate scales. Our results reiterate the need for conflict management programs to be multipronged.