Abstract: Livestock depredation by large carnivores is a global conservation challenge, and mitigation measures to reduce livestock losses are crucial for the coexistence of large carnivores and people. Various measures are employed to reduce livestock depredation but their effectiveness has rarely been tested. In this study, we tested the effectiveness of tall fences to reduce livestock losses to snow leopards Panthera uncia and wolves Canis lupus at night-time corrals at the winter camps of livestock herders in the Tost Mountains in southern Mongolia. Self-reported livestock losses at the fenced corrals were reduced from a mean loss of 3.9 goats and sheep per family and winter prior to the study to zero losses in the two winters of the study. In contrast, self-reported livestock losses in winter pastures, and during the rest of the year, when herders used different camps, remained high, which indicates that livestock losses were reduced because of the fences, not because of temporal variation in predation pressure. Herder attitudes towards snow leopards were positive and remained positive during the study, whereas attitudes towards wolves, which attacked livestock also in summer when herders moved out on the steppes, were negative and worsened during the study. This study showed that tall fences can be very effective at reducing night-time losses at corrals and we conclude that fences can be an important tool for snow leopard conservation and for facilitating the coexistence of snow leopards and people.

Abstract: The simultaneous development of technology (e.g. camera traps) and statistical methods, particularly spatially capture–recapture (SCR), has improved monitoring of large mammals in recent years. SCR estimates are known to be sensitive to sampling design, yet existing recommendations about trap spacing and coverage are often not achieved, particularly for sampling wide-ranging and rare species in landscapes that allow for limited accessibility. Consequently, most camera trap studies on large wide-ranging carnivores relies on convenience or judgmental sampling, and often yields compromised results. This study attempts to highlight the importance of carefully considered sampling design for large carnivores that, because of low densities and elusive behavior, are challenging to monitor. As a motivating example, we use two years of snow leopard camera trapping data from the same areas in the high mountains of Pakistan but with vastly different camera configurations, to demonstrate that estimates of density and space use are indeed sensitive to the trapping array. A compact design, one in which cameras were placed much closer together than generally recommended and therefore have lower spatial coverage, resulted in fewer individuals observed, but more recaptures, and estimates of density and space use were inconsistent with expectations for the region. In contrast, a diffuse design, one with larger spacing and spatial coverage and more consistent with general recommendations, detected more individuals, had fewer recaptures, but generated estimates of density and space use that were in line with expectations. Researchers often opt for compact camera configurations while monitoring wide-ranging and rare species, in an attempt to maximize the encounter probabilities. We empirically demonstrate the potential for biases when sampling a small area approximately the size of a single home range—this arises from exposing fewer individuals than deemed sufficient for estimation. The smaller trapping array may also underestimate density by significantly inflating ?. On the other hand, larger trapping array with fewer detectors and poor design induces uncertainties in the estimates. We conclude that existing design recommendations have limited utility on practical grounds for devising feasible sampling designs for large ranging species, and more research on SCR designs is required that allows for integrating biological and habitat traits of large carnivores in sampling framework. We also suggest that caution should be exercised when there is a reliance on convenience sampling.

Abstract: In countries such as Mongolia, where globalization of the cashmere market has spurred herders to massively increase their livestock numbers, an important conservation concern is the effect of livestock encroachment on wildlife. This is especially important inside protected areas (PAs), which often represent the last refugia for threatened large mammals. We used camera-traps to sample four areas with different protection status across the Mongolian Altai Mountains, and targeted a predator-prey system composed of livestock, one large herbivore, the Siberian ibex, and two large carnivores, the snow leopard and the wolf. To determine the effect of livestock on habitat use by the wild species and their spatio-temporal co-occurrence we applied an occupancy framework explicitly developed for modelling interacting species. We recorded a widespread presence of domestic animals in the PAs, and observed avoidance of sites used by livestock by snow leopard and ibex, while wolves tended to co-occur with it. Snow leopard and ibex showed clear mutual co-occurrence, indicating a tight predator-prey relationship. Results provide evidence that, at the scale of sites sampled primarily to maximise snow leopard detections, grazing livestock interferes with wild species by inducing avoidance in snow leopards, and attraction in wolves. We suggest that (1) PAs management should enforce real grazing limitations on the ground, especially in the core areas of the parks; (2) new policies incorporating wildlife conservation into government subsidies to pastoralists should be envisaged, to prevent increasing displacement of snow leopards and ibex; (3) as wolves co- occurred with livestock, with the potential for human-wildlife conflicts, we encourage the use of a set of prevention techniques to mitigate livestock depredation.

Abstract: Understanding the spatial structure of genetic diversity provides insights into a populations’ genetic status and enables assessment of its capacity to counteract the effects of genetic drift. Such knowledge is particularly scarce for the snow leopard, a conservation flagship species of Central Asia mountains. Focusing on a snow leopard population in the Qilian mountains of Gansu Province, China, we characterised the spatial genetic patterns by incorporating spatially explicit indices of diversity and multivariate analyses, based on different inertia levels of Principal Component Analysis (PCA). We compared two datasets differing in the number of loci and individuals. We found that genetic patterns were significantly spatially structured and were characterised by a broad geographical division coupled with a fine-scale cline of differentiation. Genetic admixture was detected in two adjoining core areas characterised by higher effective population size and allelic diversity, compared to peripheral localities. The power to detect significant spatial relationships depended primarily on the number of loci, and secondarily on the number of PCA axes. Spatial patterns and indices of diversity highlighted the cryptic structure of snow leopard genetic diversity, likely driven by its ability to disperse over large distances. In combination, the species’ low allelic richness and large dispersal ability result in weak genetic differentiation related to major geographical features and isolation by distance. This study illustrates how cryptic genetic patterns can be investigated and analysed at a fine spatial scale, providing insights into the spatially variable isolation effects of both geographic distance and landscape resistance.