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Jackson, R., & Fox, J. L. (1997). Snow Leopard Conservation: Accomplishments and Research Priorities. In R.Jackson, & A.Ahmad (Eds.), (pp. 128–144). Pakistan: Islt.
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Wharton, D., & Freeman, H. (1988). The Snow Leopard in North America: Captive Breeding Under the Species Survival PLan. In H.Freeman (Ed.), (pp. 131–136). India: International Snow Leoaprd Trust and WIldlife Institute of India.
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Bangjie, T., & Bingxing, Q. (1994). The Status and Problems of Snow Leopards in Captivity in China. In J.L.Fox, & D.Jizeng (Eds.), (pp. 149–156). Usa: Islt.
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Bangjie, T., & Yanfa, L. (1988). The Status of Captive Snow Leopards in China. In H.Freeman (Ed.), (pp. 151–166). India: International Snow Leopard Trust and Wildlife Institute of India.
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Trepanier, L. A., Cribb, A. E., Spielberg, S. P., & Ray, K. (1998). Deficiency of cytosolic arylamine N-acetylation in the domestic cat and wild felids caused by the presence of a single NAT1-like gene. Pharmacogenetics, 8(2), 169–179.
Abstract: The purpose of this study was to determine the molecular basis for a relative deficiency in the cat of cytosolic arylamine N- acetyltransferase (NAT), an enzyme family that is important in the metabolism of xenobiotics and that normally consists of at least two related enzymes, NAT1 and NAT2. N-acetyltransferase in feline liver showed high affinity (mean Km = 2.1 microM) for p-aminobenzoic acid, an NAT1 selective substrate in humans and rabbits, but showed a very poor affinity (mean Km > 10 mM) for sulfamethazine, an NAT2 selective substrate in humans and rabbits. Immunoreactive N-acetyltransferase was detected in feline liver, bladder and colon using an NAT1-specific antipeptide antibody, but was not detected in any tissues using an NAT2- specific antibody. Southern blot analysis of genomic DNA demonstrated a single band in domestic cats using each of six restriction digests; single bands were also found on Southern blot analysis of six wild felids. The deduced amino acid sequence of the central portion of feline N-acetyltransferase, obtained by polymerase chain reaction amplification in both domestic cats and seven wild felids (lion, tiger, lynx, snow leopard, bobcat, Asian leopard cat and cheetah), contained three residues, Phe125, Arg127, and Tyr129, which determine NAT1-like substrate specificity in humans. These results support the conclusion that cytosolic arylamine N-acetylation activity is low in the cat because of the presence of a single N-acetyltransferase that has substrate specificity, immunogenicity and sequence characteristics similar to human NAT1, and that the unusual presence of only a single N- acetyltransferase gene appears to be a family wide trait shared by other felids.
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Blomqvist, L. (1995). Three decades of Snow Leopards Panthera uncia in Captivity. Int.Zoo Yearbook, 34, 178–185.
Abstract: The author reports the status of the captive population of snow leopards over the last three decades. Genetic and demographic information is also provided. The captive population as of 1992 was 541 leopards. klf. I
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Kuzminikh, I. (1994). Notes on the status of captive snow leopards in regions of the former Soviet Union. In J.L.Fox, & D.Jizeng (Eds.), (199). Usa: Islt.
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Graham, L. H., Goodrowe, K. L., Raeside, J. I., & Liptrap, R. M. (1995). Non-invasive monitoring of ovarian function in several felid species by measurement of fecal estradiol-17-beta and progestins. Zoo Biology, 14(3), 223–237.
Abstract: An extraction and assay procedure to measure fecal estradiol-17-beta and progestin concentrations in several cat species was developed and validated for use for noninvasive monitoring of ovarian function. Fecal samples were collected over a range of 3-20 months from female tigers (three), lions (three), snow leopards (three), cheetahs (two), caracals (two), and domestic cats (five). Samples were extracted with 90% methanol, lipids removed with petroleum ether, and the estradiol and progestins in the methanol measured by radioimmunoassay (RIA). High Performance Liquid Chromatography (HPLC) fractionation and subsequent RIA of the fractions indicated that the estradiol-17-beta antiserum cross-reacted primarily with estradiol-17-beta in the feces of lions and tigers and was assumed to be specific for estradiol-17-beta in the feces of other species as well. However, there were several immunoreactive compounds, presumably progesterone metabolites, excreted in the feces which varied both quantitatively and qualitatively among species. The behavior of tigers, lions, cheetahs, and caracals was visually monitored during the collection period and frequency of sexual behaviors was positively correlated with increases in fecal estradiol in all species observed. The mean fecal estradiol-17-beta peaks were as follows: tigers, 128.0 +- 13.1; lions, 186.0 +- 14.8; snow leopards, 136.7 +- 15.9; cheetahs, 140.9 +- 9.0; caracals, 24.5 +- 4.0; and domestic cats 158.9 +- 19.3 ng/gm. Fecal progestin concentrations rose significantly (P lt 0,001) only after breeding or during pregnancy and were as follows: tigers, 5.6 +- 0.6; lions, 1.9 +- 0.1; cheetahs, 8.4 +- 1.1; and caracals, 2.4 +- 0.4 mu-g/gm. Fecal progestins were elevated for one-half to two-thirds of the gestation length during presumed pseudopregnancy but remained elevated throughout successful pregnancies. These results suggest that ovarian function can be monitored noninvasively in the family Felidae by the measurement of fecal estradiol-17-beta and progestin concentrations.
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Roth, T. L., Swanson, W. F., & Wildt, D. E. (1995). Snow leopard (Panthera unica) sperm longevity in vitro is not influenced by protein or energy source supplements but is affected by buffer source. Theriogenology, 43(1), 309.
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Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. (2014). Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples. Conservation Genetic Resource, 6(2), 369:373.
Abstract: Molecular markers that can effectively identify noninvasively collected samples and provide genetic
information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many
noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the
domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard
populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and
population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers
facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.
endangered species
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