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| Author | Lama, R. P., Ghale, T. R., Suwal, M. K., Ranabhat, R., Regmi, G. R. | ||||
| Title | First photographic evidence of Snow Leopard Panthera uncia (Mammalia: Carnivora: Felidae) outside current protected areas network in Nepal Himalaya | Type | Journal Article | ||
| Year | 2018 | Publication | Journal of Threatened Taxa | Abbreviated Journal | |
| Volume | Issue | Pages  |
12086-12090 | ||
| Keywords | Camera-trapping, conservation, Humla, livestock depredation, monasteries, non-timber forest products, retaliatory killing, Tibetan Buddhism. | ||||
| Abstract | The Snow Leopard Panthera uncia is a rare top predator of high-altitude ecosystems and insufficiently surveyed outside of protected areas in Nepal. We conducted a rapid camera-trapping survey to assess the presence of Snow Leopard in the Limi valley of Humla District. Three individuals were recorded in two camera locations offering the first photographic evidence of this elusive cat outside the protected area network of Nepal. In addition to Snow Leopard, the Blue Sheep Pseudois nayaur, Beech Marten Martes foina, Pika Ochotona spp. and different species of birds were also detected by camera-traps. More extensive surveys and monitoring are needed for reliably estimating the population size of Snow Leopard in the area. The most urgent needs are community-based conservation activities aimed at mitigating immediate threats of poaching, retaliatory killing, and rapid prey depletion to ensure the survival of this top predator in the Himalaya. | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rakhee @ | Serial | 1470 | ||
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| Author | Johnston, L.A.; Donoghue, A.M.; O'Brien, S.J.; Wildt, D.E. | ||||
| Title | Rescue and maturation in vitro of follicular oocytes collected from nondomestic felid species | Type | Journal Article | ||
| Year | 1991 | Publication | Biol Reprod | Abbreviated Journal | |
| Volume | 45 | Issue | 6 | Pages |
898-906 |
| Keywords | Animal; Carnivora; anatomy; histology; physiology; Cells; Cultured; Female; Fertilization; Vitro; inVitro; Male; Oocytes; cytology; growth; development; ovarian; Follicle; Spermatozoa; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 230 | ||||
| Abstract | The potential for rescuing immature oocytes from the ovaries of females of rare felid species which die or undergo medical ovariohysterectomy was evaluated. Ovaries were recovered from 13 species representing 35 individuals in good-to-poor health. Although the majority of females were 10 yr of age or older and in fair-to-poor health, a total of 846 oocytes were recovered of which 608 (71.9%) were classified as fair-to- excellent quality. One hundred of these oocytes were used for initial maturation classification and as parthogenetic controls. Overall, of the 508 fair-to-excellent quality oocytes placed in culture, 164 (32.3%) matured to metaphase II in vitro. For species in which 3 or more individuals yielded oocytes, mean oocyte maturation rates were as follows: 36.2%, tiger; 27.9% leopard; and 8.3%, cheetah. In vitro insemination of oocytes resulted in fertilization (2 polar bodies, 2 pronuclei, or cleavage) rates of 9.1% to 28.6% (leopard) using homologous fresh spermatozoa and 4.0% (lion) to 40.0% (puma) using homologous frozen-thawed spermatozoa. Inseminations using heterologous (domestic cat) spermatozoa also resulted in fertilized oocytes in the tiger, leopard, snow leopard, puma, serval, and Geoffroy's cat (range in fertilization rate, 5.0% for leopard to 46.2% for puma). Cleaved embryos resulted from the insemination of leopard oocytes with homologous sperm (n = 1 embryo) and puma oocytes with domestic cat sperm (n = 3 embryos). These results demonstrate that immature ovarian oocytes from rare felid species can be stimulated to mature in vitro despite an excision-to-culture interval as long as 36 h.(ABSTRACT TRUNCATED AT 250 WORDS) | ||||
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| ISSN | 0006-3363 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 172 | Serial | 503 | ||
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| Author | Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. | ||||
| Title | Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces | Type | Journal Article | ||
| Year | 1994 | Publication | Biol Reprod | Abbreviated Journal | |
| Volume | 51 | Issue | 4 | Pages |
776-786 |
| Keywords | Animal; Carbon; Radioisotopes; Carnivora; Cats; Chromatography; High; Pressure; Liquid; Comparative Study; Estradiol; metabolism; Estrone; feces; chemistry; Female; Ovary; physiology; Pregnancy; Progesterone; Pseudopregnancy; Support; Non-U.S.Gov't; browse; non; government; gov't; us; 170 | ||||
| Abstract | Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species. | ||||
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| ISSN | 0006-3363 | ISBN | Medium | ||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 251 | Serial | 198 | ||
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| Author | Ale, S.; Whelan, C. | ||||
| Title | Reappraisal of the role of big, fierce predators | Type | Miscellaneous | ||
| Year | 2008 | Publication | Biodiversity Conservation | Abbreviated Journal | |
| Volume | Issue | Pages |
685-690 | ||
| Keywords | Biodiversity ú Conservation ú Costs of predation ú Indirect effects ú Non-lethal effects ú Predators ú Top-down control; big; predators; predator | ||||
| Abstract | The suggestion in the early 20th century that top predators were a necessary component of ecosystems because they hold herbivore populations in check and promote biodiversity was at Wrst accepted and then largely rejected. With the advent of Evolutionary Ecology and a more full appreciation of direct and indirect effects of top predators, this role of top predators is again gaining acceptance. The previous views were predicated upon lethal effects of predators but largely overlooked their non-lethal effects. We suggest that conceptual advances coupled with an increased use of experiments have convincingly demonstrated that prey experience costs that transcend the obvious cost of death. Prey species use adaptive behaviours to avoid predators, and these behaviours are not cost-free. With predation risk, prey species greatly restrict their use of available habitats and consumption of available food resources. Effects of top predators consequently cascade down to the trophic levels below them. Top predators, the biggies, are thus both the targets of and the means for conservation at the landscape scale. |
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| Language | English | Summary Language | Original Title | ||
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ 885 | Serial | 52 | ||
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| Author | Roth, T.L.; Howard, J.G.; Donoghue, A.M.; Swanson, W.F.; Wildt, D.E. | ||||
| Title | Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro | Type | Journal Article | ||
| Year | 1994 | Publication | J Reprod Fertil | Abbreviated Journal | |
| Volume | 101 | Issue | 3 | Pages |
563-569 |
| Keywords | Animal; Carnivora; physiology; Cell; survival; Cells; Cultured; Comparative; study; Culture; Media; Female; Male; Pentoxifylline; Sperm; Motility; Ovum; Interactions; Spermatozoa; cytology; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 190 | ||||
| Abstract | Electroejaculates from eight snow leopards were used to determine how the motility of spermatozoa was influenced by (i) type of media (Ham's F10, PBS, human tubal fluid or RPMI-1640); (ii) holding temperature (23 degrees C versus 37 degrees C); (iii) washing of spermatozoa and (iv) a sperm metabolic enhancer, pentoxifylline. The duration of sperm motility was assessed by evaluating samples in each treatment every hour for 6 h and a sperm motility index (a value combining percentage sperm motility and rate of forward progression) calculated. Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. During the 6 h co-incubation, the sperm motility index in PBS with pentoxifylline was greater (P < 0.05) than in PBS alone which, in turn, was greater (P < 0.05) than in the other three test media. Washing the spermatozoa enhanced (P < 0.05) motility in both PBS and PBS plus pentoxifylline relative to unwashed samples, but there was no effect (P > 0.05) of holding temperature. Pentoxifylline supplementation enhanced (P < 0.05) the proportion of cat eggs with bound, but not penetrated, snow leopard spermatozoa in the inner layer of the zona pellucida, and there were no spermatozoa in the perivitelline space.(ABSTRACT TRUNCATED AT 250 WORDS) | ||||
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| ISSN | 0022-4251 | ISBN | Medium | ||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 253 | Serial | 828 | ||
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| Author | Janecka; J.E.; Jackson, R.; Yuquang, Z.; Diqiang, L.; Munkhtsog, B.; Buckley-Beason, V.; Murphy, W.J. | ||||
| Title | Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study | Type | Miscellaneous | ||
| Year | 2008 | Publication | Animal Conservation | Abbreviated Journal | |
| Volume | 11 | Issue | Pages |
401-411 | |
| Keywords | snow leopard; genetics; scat; noninvasive; survey. | ||||
| Abstract | The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards. | ||||
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| Notes | Supplemental information may be found at http:snowleopardnetwork.org/bibliography/JaneckaSupplemental2008.pdf | Approved | no | ||
| Call Number | SLN @ rana @ 883 | Serial | 488 | ||
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| Author | Darehshuri, B.F. | ||||
| Title | Threatened cats of Asia | Type | Journal Article | ||
| Year | 1978 | Publication | Wildlife | Abbreviated Journal | |
| Volume | 20 | Issue | 9 | Pages |
396-400 |
| Keywords | Asia, Acinonyx jubatus, Asiatic cheetah, cheetah, Siberian tiger, snow leopard | ||||
| Abstract | Man's hand is turned against the wild cats wherever they occur, often due to the value of their fur, but also because of the danger they sometimes pose to domestic stock and even human beings. All the larger Asian cats are threatened, and on this and the following pages we look at three of them – the Asiatic cheetah, the Siberian tiger, and the snow leopard. | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ | Serial | 1116 | ||
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| Author | Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. | ||||
| Title | Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples | Type | Journal Article | ||
| Year | 2014 | Publication | Conservation Genetic Resource | Abbreviated Journal | |
| Volume | 6 | Issue | 2 | Pages |
369:373 |
| Keywords | Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification | ||||
| Abstract | Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. |
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| Notes | Approved | no | |||
| Call Number | SLN @ rakhee @ | Serial | 1428 | ||
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| Author | Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. | ||||
| Title | Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples | Type | Journal Article | ||
| Year | 2014 | Publication | Conservation Genetic Resource | Abbreviated Journal | |
| Volume | 6 | Issue | 2 | Pages |
369:373 |
| Keywords | Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification | ||||
| Abstract | Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. endangered species |
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| Notes | Approved | no | |||
| Call Number | SLN @ rakhee @ | Serial | 1427 | ||
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| Author | Chapron, G. | ||||
| Title | Re-wilding: other projects help carnivores stay wild | Type | Journal Article | ||
| Year | 2005 | Publication | Nature | Abbreviated Journal | |
| Volume | 437 | Issue | Pages |
318 | |
| Keywords | Acinonyx jubatus, carnivore, coexistence, conservation, damage prevention, Panthera leo, snow leopard, survival, Uncia uncia | ||||
| Abstract | Letter to Nature Editor, in response to: In their plea for bringing Pleistocene wildlife to the New World (“Re-wilding North America” Nature 436, 913–914; 2005), Josh Donlan and colleagues do not discuss successful efforts to ensure long-term survival of large carnivores in Africa and Asia. A few examples are given. | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ | Serial | 1114 | ||
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