Mukherjee, S., Ashalakshmi, C. N., Home, C., Ramakrishnan, U. (2010). SAhonrt Reepvoartluation of the PCR-RFLP technique to aid molecular-based monitoring of felids and canids in India. BMC Research Notes, 3, 159–166.
Abstract: Background: The order Carnivora is well represented in India, with 58 of the 250 species found globally, occurring here. However, small carnivores figure very poorly in research and conservation policies in India. This is mainly due to
the dearth of tested and standardized techniques that are both cost effective and conducive to small carnivore studies in the field. In this paper we present a non-invasive genetic technique standardized for the study of Indian felids and canids with the use of PCR amplification and restriction enzyme digestion of scat collected in the field.
Findings: Using existing sequences of felids and canids from GenBank, we designed primers from the 16S rRNA region of the mitochondrial genome and tested these on ten species of felids and five canids. We selected restriction enzymes that would cut the selected region differentially for various species within each family. We produced a restriction digestion profile for the potential differentiation of species based on fragment patterns. To test our technique, we used felid PCR primers on scats collected from various habitats in India, representing varied environmental conditions. Amplification success with field collected scats was 52%, while 86% of the products used for restriction digestion could be accurately assigned to species. We verified this through sequencing. A comparison of costs across the various techniques currently used for scat assignment showed that this technique was the most practical and cost effective.
Conclusions: The species-specific key developed in this paper provides a means for detailed investigations in the future that focus on elusive carnivores in India and this approach provides a model for other studies in areas of Asia
where many small carnivores co-occur.
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Berg L.S. (1938). Fauna.
Abstract: It provides description of fauna of the Central Asia mountains. Ibex (Capra sibirica) was noticed to keep to the alpine and sub-alpine zone and never descends bellow 2,500 m. Hunting for ibex and wild sheep, snow leopard (Leopardus uncia) keeps at the same elevation.
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Jackson, R., & Ahlborn, G. (1989). Snow leopards (Panthera- uncia) in Nepal – home range and movements. National Geographic Research, 5(2), 161–175.
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Jackson, R., & Ahlborn, G. (1989). Snow Leopards in Nepal-home range and movements. National Geographic Res., 5, 161–175.
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Kuznetsov B.A. (1975). The cats – Felidae (Vol. Part 3.).
Abstract: An identification table for genus and species of Felidae family of USSR is given. Snow leopard Panthera uncia (Schreher) described for Pamir, Tien Shan, Altai and mountains of Tuva.
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Yazan Yu.P. (1980). The Almaty state nature reserve.
Abstract: History of the Alma-Ata nature reserve establishment, its location physical and geographic data, climate, and vegetation zones are described. Fauna of the protected area is represented by 64 mammal species, and about 200 bird species. Briefly described are behavioural patterns of roe deer, moral, wild boar, Siberian ibex, snow leopard, lynx, brown bear, and marten, and other mammals of the mountainous area.
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Van Bree, P. J. H. (1965). Zum habitus neugeborener Schneeleoparden, Uncia uncia (Schreber, 1775). Saugetierkundliche Mitteilungen, 13, 162–163.
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Jalanka, H. H. (1989). Evaluation and comparison of 2 ketamine-based immobilization techniques in snow leopards (Panthera uncia). Journal of Zoo and Wildlife Medicine, 20(2), 163–169.
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Jizhong, Z. (1994). A preliminary study on the care and breeding of snow leopard in Jinan Zoo, China. In J.L.Fox, & D.Jizeng (Eds.), (pp. 163–165). Usa: Islt.
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Ochilov A.M. (1986). Protected areas of Kashkadarya province.
Abstract: The 30 mammals, 180 birds, 9 reptiles and 1 fish are presented in Hissar nature reserve, Uzbekistan. Snow leopard, Turkestan lynx, brown bear, otter, golden eagle and Himalayan griffon included in IUCN Red data Book. Number of ibex was declined.
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