Abstract: The protection of Snow Leopards in the remote and economically disadvantaged Northern Areas of Pakistan needs local people equipped with the skills to gather and present information on the number and range of individual animals in their area. It is important for the success of a conservation campaign that the people living in the area are engaged in the conservation process. Snow Leopards are elusive and range through inhospitable terrain so direct study is difficult. Consequently the major goals for this project were twofold, to gather information on snow leopard distribution in this area and to train local university students and conservation management professionals in the techniques used for locating snow leopards without the need to capture or even see the animals. This project pioneered the use of DNA testing of field samples collected in Pakistan to determine the distribution of snow leopards and to attempt to identify individuals. These were collected in and around that country's most northerly national park, the Kunjurab National Park, which sits on the Pakistan China border. Though the Northern Areas is not a well developed part of Pakistan, it does possess a number of institutions that can work together to strengthen snow leopard conservation. The first of these is a newly established University with students ready to be trained in the skills needed. Secondly WWF-Pakistan has an office in the main town and a state of the art GIS laboratory in Lahore and already works closely with the Forest Department who manage the national park. All three institutions worked together in this project with WWF providing GIS expertise, the FD rangers, and the university students carrying out the laboratory work. In addition in the course of the project the University of the Punjab in Lahore also joined the effort, providing laboratory facilities for the students. As a result of this project maps have been produced showing the location of snow leopards in
two areas. Preliminary DNA evidence indicates that there is more than one animal in this
relatively small area, but the greatest achievement of this project is the training and
experience gained by the local students. For one student this has been life changing. Due to
the opportunities provided by this study the student, Nelofar gained significant scientific
training and as a consequence she is now working as a lecturer and research officer for the
Center for Integrated Mountain Research, New Campus University of the Punjab, Lahore
Pakistan

Abstract: Between May 1990 and January 1991, behavioral observations were made of two captive pumas (Felis concolor Linnaeus), and two captive snow leopards (Panthera uncia Schreber) in their outdoor exhibits at the Lincoln Park Zoological Gardens, Chicago, Illinois. Behaviors compared within and between species included: 1) time spend in the different habitat types; 2) time budgets for the different behaviors: laying, moving, sitting, standing, crouching, in the tree, drinking, urinating, defecating, within their inside dens, and “behavior not determined” when the identity or behavior of the individuals could not be determined; and 3) mobility of the animals within their exhibits. Also examined were: 4) preferences for different habitat types; 5) recommendations for future exhibit designs. Both species located themselves within their exhibits in a non-random manner. The majority of cats' time was spent in elevated locations (i.e., gunite ledges approximately 1-5.5 m above ground-level). Snow leopards exhibited this tendency to a greater extent than did the pumas. Both species also spent the majority of their time in the lying-down behavior; again snow leopards displayed this tendency significantly more than the pumas. Pumas were highly mobile and changed locations and behaviors in their exhibit significantly more than the snow leopards. No significant differences were noted between conspecifics in regard to habitat type preference, or mobility within the exhibit. Suggestions for future exhibit design include elevated locations for the cats to lay and look around within and outside their exhibits, caves for access to shade or relief from inclement weather, and ground surfaces to move about on. Features for exhibit design should take into consideration the natural habitat of the cat to occupy the exhibit.

Abstract: The complete mitochondrial genome (mtDNA) of snow leopard Panthera uncia was obtained by using the polymerase chain reaction (PCR) technique based on the PCR fragments of 30 primers we designed. The entire mtDNA sequence was 16 773 base pairs (bp) in length, and the base composition was: A-5,357ª“,Ž+bp (31.9%); C-4,444ª”,Ž+bp (26.5%); G-2,428ª“,Ž+bp (14.5%); T-4,544ª”,Ž+bp (27.1%). The structural characteristics [0] of the P. uncia mitochondrial genome were highly similar to these of Felis catus, Acinonyx jubatus, Neofelis nebulosa and other mammals. However, we found several distinctive features of the mitochondrial genome of Panthera unica. First, the termination codon of COIII was TAA, which differed from those of F. catus, A. jubatus and N. nebulosa. Second, tRNASer (AGY), which lacked the ''DHU'' arm, could not be folded into the typical cloverleaf-shaped structure. Third, in the control region, a long repetitive sequence in RS-2 (32ª“,Ž+bp) region was found with 2 repeats while one short repetitive segment (9ª”,Ž+bp) was found with 15 repeats in the RS-3 region. We performed phylogenetic analysis based on a 3 816ª",Ž+bp concatenated sequence of 12S rRNA, 16S rRNA, ND2, ND4, ND5, Cyt b and ATP8 for P. uncia and other related species, the result indicated that P. uncia and P. leo were the sister species, which was different from the previous findings. (c) 2008 Springer Science+Business Media B.V.

Abstract: In the cold and arid mountains of Central Asia, where the diversity and abundance of wild ungulates
are generally low, resource partitioning among coexisting carnivores is probably less distinct than in prey-rich areas. Thus, similar-sized carnivores are likely to compete for food. We compared the summer diets of snow leopards Panthera uncia and wolves Canis lupus in Sarychat-Ertash Reserve in the Tien-Shan mountains of Kyrgyzstan, based on analysis of genetically confirmed scats. Abundances of
the principal prey species, argali Ovis ammon and Siberian ibex Capra sibirica, were estimated from field surveys. The diets consisted of few species, with high interspecific overlap (Pianka’s index50.91). Argali was the predominant prey, with .50% frequency of occurrence in both snow leopard and wolf scats. This was followed by Siberian ibex and marmots Marmota baibacina. Being largely unavailable, remains of livestock were not detected in any of the scats. In the snow leopard diet, proportions of argali and ibex were in
line with the relative availabilities of these animals in the Reserve. This was in contrast to the diet of wolf, where argali occurred according to availability and ibex was significantly underrepresented. The high diet overlap indicates that the two predators might compete for food when the diversity of profitable, large prey is low. Competition may be more intense in winter, when marmots are not available. Hunting of argali and ibex outside the Reserve may be unsustainable and therefore reduce their abundances over time. This will
affect both predators negatively and intensify competition for food. Reduction in ibex populations will directly affect the snow leopard, and the wolf is likely to be indirectly affected as a result of increased snow leopard predation of argali.

Abstract: The threat posed by large carnivores to livestock and humans makes peaceful coexistence between
them difficult. Effective implementation of conservation laws and policies depends on the attitudes of local
residents toward the target species. There are many known correlates of human attitudes toward carnivores,
but they have only been assessed at the scale of the individual. Because human societies are organized hierarchically, attitudes are presumably influenced by different factors at different scales of social organization, but this scale dependence has not been examined.We used structured interview surveys to quantitatively assess the attitudes of a Buddhist pastoral community toward snow leopards (Panthera uncia) and wolves (Canis lupus).
We interviewed 381 individuals from 24 villages within 6 study sites across the high-elevation Spiti Valley in
the Indian Trans-Himalaya. We gathered information on key explanatory variables that together captured
variation in individual and village-level socioeconomic factors.We used hierarchical linear models to examine how the effect of these factors on human attitudes changed with the scale of analysis from the individual to the community. Factors significant at the individual level were gender, education, and age of the respondent (for wolves and snow leopards), number of income sources in the family (wolves), agricultural production, and large-bodied livestock holdings (snow leopards). At the community level, the significant factors included the number of smaller-bodied herded livestock killed by wolves and mean agricultural production (wolves) and village size and large livestock holdings (snow leopards). Our results show that scaling up from the individual to higher levels of social organization can highlight important factors that influence attitudes of people toward wildlife and toward formal conservation efforts in general. Such scale-specific information can help managers apply conservation measures at appropriate scales. Our results reiterate the need for conflict management programs to be multipronged.