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Author Janecka; J.E.; Jackson, R.; Yuquang, Z.; Diqiang, L.; Munkhtsog, B.; Buckley-Beason, V.; Murphy, W.J.
Title Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study Type Miscellaneous
Year 2008 Publication Animal Conservation Abbreviated Journal
Volume 11 Issue Pages 401-411
Keywords snow leopard; genetics; scat; noninvasive; survey.
Abstract The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards.
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Notes Supplemental information may be found at http:snowleopardnetwork.org/bibliography/JaneckaSupplemental2008.pdf Approved no
Call Number SLN @ rana @ 883 Serial 488
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Author Jack, R.
Title DNA Testing and GPS positioning of snow leopard (Panthera uncia) genetic material in the Khunjerab National Park Northern Areas, Pakistan Type Report
Year 2008 Publication Abbreviated Journal
Volume Issue Pages 1-15
Keywords project; snow; snow leopard; snow-leopard; leopard; network; conservation; program; Dna; Gps; panthera; panthera uncia; Panthera-uncia; uncia; Khunjerab; Khunjerab-National-Park; national; national park; National-park; park; areas; area; Pakistan; protection; snow leopards; snow-leopards; leopards; local; local people; people; information; number; range; Animals; Animal; study; distribution; management; professional; techniques; capture; use; field; country; China; border; work; art; Gis; Forest; manage; Wwf; maps; map; location; training; research; mountain
Abstract The protection of Snow Leopards in the remote and economically disadvantaged Northern Areas of Pakistan needs local people equipped with the skills to gather and present information on the number and range of individual animals in their area. It is important for the success of a conservation campaign that the people living in the area are engaged in the conservation process. Snow Leopards are elusive and range through inhospitable terrain so direct study is difficult. Consequently the major goals for this project were twofold, to gather information on snow leopard distribution in this area and to train local university students and conservation management professionals in the techniques used for locating snow leopards without the need to capture or even see the animals. This project pioneered the use of DNA testing of field samples collected in Pakistan to determine the distribution of snow leopards and to attempt to identify individuals. These were collected in and around that country's most northerly national park, the Kunjurab National Park, which sits on the Pakistan China border. Though the Northern Areas is not a well developed part of Pakistan, it does possess a number of institutions that can work together to strengthen snow leopard conservation. The first of these is a newly established University with students ready to be trained in the skills needed. Secondly WWF-Pakistan has an office in the main town and a state of the art GIS laboratory in Lahore and already works closely with the Forest Department who manage the national park. All three institutions worked together in this project with WWF providing GIS expertise, the FD rangers, and the university students carrying out the laboratory work. In addition in the course of the project the University of the Punjab in Lahore also joined the effort, providing laboratory facilities for the students. As a result of this project maps have been produced showing the location of snow leopards in

two areas. Preliminary DNA evidence indicates that there is more than one animal in this

relatively small area, but the greatest achievement of this project is the training and

experience gained by the local students. For one student this has been life changing. Due to

the opportunities provided by this study the student, Nelofar gained significant scientific

training and as a consequence she is now working as a lecturer and research officer for the

Center for Integrated Mountain Research, New Campus University of the Punjab, Lahore

Pakistan
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Notes Project funded by Snow Leopard Network's Snow Leopard Conservation Grant Program. Approved no
Call Number SLN @ rana @ 1067 Serial 427
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Author Espinosa-Aviles, D.; Taylor, M.L.; Del Rocio Reyes-Montes, M.; Pe'rez-Torrez, A.
Title Molecular findings of disseminated histoplasmosis in two captive snow leopards (Uncia uncia) Type Miscellaneous
Year 2008 Publication Journal-of-Zoo-and-Wildlife-Medicine Abbreviated Journal
Volume 39 Issue Pages 450-454
Keywords captive; histoplasmosis; Immunohistochemistry; Molecular; snow leopard; Uncia uncia
Abstract This paper reports two cases of disseminated histoplasmosis in captive snow leopards (Uncia uncia). Histoplasmosis was diagnosed based on histopathology, immunohistochemistry, transmission electron microscopy, and molecular findings.
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Call Number SLN @ rana @ 907 Serial 268
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Author Chalise, M.K.
Title Nepalka Samrakshit Banyajantu (Nepal's Protected Wildlife in Nepali language) Type Book Whole
Year 2008 Publication Abbreviated Journal
Volume Issue Pages 106-108
Keywords government; language; leopard; leopards; Nepal; protected; snow; snow-leopard; snow-leopards; snow leopard; snow leopards; wildlife
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Publisher Shajha Prakashan Place of Publication Lalitpur, Kathmandu Editor
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Notes In Nepalese language only. Includes only the chapter on snow leopards and the book cover. The book is published by a government corporate house of publication. Approved no
Call Number SLN @ rana @ 1058 Serial 211
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Author Burgener, N.; Gusset, M.; Schmid, H.
Title Frustrated appetitive foraging behavior, stereotypic pacing, and fecal glucocorticoid levels in snow leopards (Uncia uncia) in the Zurich Zoo Type Miscellaneous
Year 2008 Publication Journal of Applied Animal Welfare Science Abbreviated Journal
Volume 11 Issue Pages 74-83
Keywords behavior; captive; fecal; feeding strategy; physiological; snow leopard; zoo
Abstract This study hypothesized that permanently frustrated, appetitive-foraging behavior caused the stereotypic pacing regularly observed in captive carnivores. Using 2 adult female snow leopards (Uncia uncia), solitarily housed in the Zurich Zoo, the study tested this hypothesis experimentally with a novel feeding method: electronically controlled, time-regulated feeding boxes. The expected result of employing this active foraging device as a successful coping strategy was reduced behavioral and physiological measures of stress, compared with a control-feeding regime without feeding boxes. The study assessed this through behavioral observations and by evaluating glucocorticoid levels noninvasively from feces. Results indicated that the 2 snow leopards did not perform successful coping behavior through exercising active foraging behavior or through displaying the stereotypic pacing. The data support a possible explanation: The box-feeding method did not provide the 2 snow leopards with the external stimuli to satisfy their appetitive behavioral needs. Moreover, numerous other factors not necessarily or exclusively related to appetitive behavior could have caused and influenced the stereotypic pacing.
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Call Number SLN @ rana @ 915 Serial 202
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Author Broder, J.; MacFadden, A.; Cosens, L.; Rosenstein, D.; Harrison, T.
Title Use of Positive Reinforcement Conditioning to Monitor Pregnancy in an Unanesthetized Snow Leopard (Uncia uncia) via Transabdominal Ultrasound Type Miscellaneous
Year 2008 Publication Zoo Biology Abbreviated Journal
Volume 27 Issue Pages 78-85
Keywords desensitization; fetal development; operant conditioning; pregnancy detection; primiparous; snow leopard; zoo
Abstract Closely monitoring snow leopard (Uncia uncia) fetal developments via transabdominal ultrasound, with minimal stress to the animal, was the goal of this project. The staff at Potter Park Zoo has used the principles of habituation, desensitization, and positive reinforcement to train a female snow leopard (U. uncia). Ultrasound examinations were preformed on an unanesthetized feline at 63 and 84 days. The animal remained calm and compliant throughout both procedures. Fetuses were observed and measured on both occasions. The absence of anesthesia eliminated components of psychologic and physiologic stress associated with sedation. This was the first recorded instance of transabdominal ultrasound being carried out on an unanesthetized snow leopard. It documents the feasibility of detecting pregnancy and monitoring fetal development via ultrasound.
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Call Number SLN @ rana @ 905 Serial 196
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Author Blomqvist, L.
Title The status of the snow leopard in the EEP – program in 2007 Type Book Chapter
Year 2008 Publication International Pedigree Book of Snow Leopards Abbreviated Journal
Volume 9 Issue 4 Pages 20-24
Keywords status; snow; snow leopard; snow-leopard; leopard; program; International; pedigree; snow leopards; snow-leopards; leopards
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Publisher Helsinki Zoo Place of Publication Helsinki Editor Blomqvist, L.
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Notes Approved no
Call Number SLN @ rana @ 1007 Serial 174
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Author Bhatnagar, Y.V.
Title Relocation from wildlife reserves in the Greater and Trans-Himalayas: Is it necessary? Type Miscellaneous
Year 2008 Publication Conservation and Society Abbreviated Journal
Volume 6 Issue 3 Pages 263-270
Keywords wildlife reserves,relocation,Greater Himalayas,Trans-Himalayas; wildlife; reserves; relocation; Himalayas; Himalaya; trans-himalaya; transhimalaya
Abstract The Greater and Trans-Himalayan tracts are cold deserts that have severe seasonal and resource scarce environments. Covering the bulk of Indian Himalayas, they are a rich repository of biodiversity values and ecosystem services. The region has a large protected area (PA) network which has not been completely effective in conserving these unique values. The human population densities are much lower (usually < 1 per sq km) than in most other parts of the country (over 300 to a sq km). However, even such small populations can come into conflict with strict PA laws that demand large inviolate areas, which can mainly be achieved through relocation of the scattered settlements. In this paper, I reason that in this landscape relocation is not a tenable strategy for conservation due to a variety of reasons. The primary ones are that wildlife, including highly endangered ones are pervasive in the larger landscape (unlike the habitat 'islands' of the forested ecosystems) and existing large PAs usually encompass only a small proportion of this range. Similarly, traditional use by people for marginal cultivation, biomass extraction and pastoralism is also as pervasive in this landscape. There does exist pockets of conflict and these are probably increasing owing to a variety of changes relating to modernisation. However, scarce resources, the lack of alternatives and the traditional practice of clear-cut division of all usable areas and pastures between communities make resettlement of people outside PAs extremely difficult. It is reasoned that given the widespread nature of the wildlife and pockets of relatively high density, it is important to prioritise these smaller areas for conservation in a scenario where they form a mosaic of small 'cores' that are more effectively maintained with local support and that enable wildlife to persist. These ideas have recently gained widespread acceptance in both government and conservation circles and may soon become part of national strategy for these areas.
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Call Number SLN @ rana @ 914 Serial 138
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Author Williams, N.
Title 2008 International Conference on Range-wide Conservation Planning for Snow Leopards: Saving the Species Across its Range Type Journal Article
Year 2008 Publication Cat News Abbreviated Journal
Volume 48 Issue Pages 33-34
Keywords Panthera, conference,Beijing, China, 2008, planning, Wildlife Conservation Society, Snow Leopard Trust, Snow Leopard Network, Chinese Institute of Zoology
Abstract Over 100 snow leopard experts, enthusiasts, and government officials gathered in the outskirts of Beijing, China from March 7–11, 2008 for the firstever International Conference on Range-wide Conservation Planning for Snow Leopards. Conference organizers included Panthera, Wildlife Conservation Society (WCS), Snow Leopard Trust (SLT), Snow Leopard Network (SLN), and the Chinese Institute of Zoology.
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Call Number SLN @ rana @ Serial 1344
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Author Tytar, V.; Hammer, M.
Title Expedition report: Mountain ghosts: snow leopards and other animals in the mountains of the Altai Republic, Central Asia Type Report
Year 2008 Publication Abbreviated Journal
Volume Issue Pages
Keywords Altai
Abstract This study was part of an expedition to the Altai mountains in the Kosh Agach region of the Altai Republic, run by Biosphere Expeditions from 8 July to 17 August 2007. The aim was to continue a survey of snow leopard (Uncia uncia) in this area, as well as surveying the snow leopard's primary prey species, argali (Ovis ammon) and Siberian ibex (Capra sibirica), together with secondary prey species.

Using the Snow Leopard Information Management System (SLIMS) developed by the International Snow Leopard Trust (ISLT), presence/absence surveys (SLIMS form 1) of snow leopard and prey species were conducted throughout the study period across the entire survey area (approximately 200 square kilometers). In 2007 surveys were extended to areas away from the Tapduair massif site to the valley and surrounding ridges of Irbistu mountain. Interviews with local, semi-nomadic herders also formed an important part of the research procedure. The expedition also collected data for extended mammal, bird and plant inventories.

The decrease in numbers of the primary prey species observed recently makes it very likely that food availability is not in favour of the snow leopard in the study area. This is supported by the fact that in 2006 there were no records of snow leopard sign in the core area and in 2007 only a few were found.

Nevertheless, the study area retains its importance as a habitat for snow leopard and as a corridor for snow leopard dispersal. Unfortunately, privatisation of formerly common land is on the increase as is a lack of respect traditional land management practices, so the survey area urgently needs protection, but involving the local community and raising public awareness is vital if conservation initiatives are to succeed.
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Notes Expedition dates: 8 July – 17 August 2007 Approved no
Call Number SLN @ rana @ Serial 1143
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