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| Author | Janecka; J.E.; Jackson, R.; Yuquang, Z.; Diqiang, L.; Munkhtsog, B.; Buckley-Beason, V.; Murphy, W.J. | ||||
| Title | Population monitoring of snow leopards using noninvasive collection of scat samples: a pilot study | Type | Miscellaneous | ||
| Year | 2008 | Publication | Animal Conservation | Abbreviated Journal | |
| Volume | 11 | Issue | Pages | 401-411 | |
| Keywords | snow leopard; genetics; scat; noninvasive; survey. | ||||
| Abstract | The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards. | ||||
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| Notes | Supplemental information may be found at http:snowleopardnetwork.org/bibliography/JaneckaSupplemental2008.pdf | Approved | no | ||
| Call Number | SLN @ rana @ 883 | Serial | 488 | ||
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| Author | Korablev, M. P., Poyarkov, A. D., Karnaukhov, A. S., Zvychaynaya, E. Y., Kuksin, A. N., Malykh, S. V., Istomov, S. V., Spitsyn, S. V., Aleksandrov, D. Y., Hernandez-Blanco, J. A., Munkhtsog, B., Munkhtogtokh, O., Putintsev, N. I., Vereshchagin, A. S., Becmurody, A., Afzunov, S., Rozhnov, V. V. | ||||
| Title | Large-scale and fine-grain population structure and genetic diversity of snow leopards (Panthera uncia Schreber, 1776) from the northern and western parts of the range with an emphasis on the Russian population. | Type | Journal Article | ||
| Year | 2021 | Publication | Conservation Genetics | Abbreviated Journal | |
| Volume | Issue | Pages | |||
| Keywords | Snow leopard, Panthera uncia, Microsatellites, Heterozygosity, Population structure, Noninvasive survey, Scat, Subspecies | ||||
| Abstract | The snow leopard (Panthera uncia Schreber, 1776) population in Russia and Mongolia is situated at the northern edge of the range, where instability of ecological conditions and of prey availability may serve as prerequisites for demographic instability and, consequently, for reducing the genetic diversity. Moreover, this northern area of the species distribution is connected with the western and central parts by only a few small fragments of potential habitats in the Tian-Shan spurs in China and Kazakhstan. Given this structure of the range, the restriction of gene flow between the northern and other regions of snow leopard distribution can be expected. Under these conditions, data on population genetics would be extremely important for assessment of genetic diversity, population structure and gene flow both at regional and large-scale level. To investigate large-scale and fine-grain population structure and levels of genetic diversity we analyzed 108 snow leopards identified from noninvasively collected scat samples from Russia and Mongolia (the northern part of the range) as well as from Kyrgyzstan and Tajikistan (the western part of the range) using panel of eight polymorphic microsatellites. We found low to moderate levels of genetic diversity in the studied populations. Among local habitats, the highest heterozygosity and allelic richness were recorded in Kyrgyzstan (He = 0.66 ± 0.03, Ho = 0.70 ± 0.04, Ar = 3.17) whereas the lowest diversity was found in a periphery subpopulation in Buryatia Republic of Russia (He = 0.41 ± 0.12, Ho = 0.29 ± 0.05, Ar = 2.33). In general, snow leopards from the western range exhibit greater genetic diversity (He = 0.68 ± 0.04, Ho = 0.66 ± 0.03, Ar = 4.95) compared to those from the northern range (He = 0.60 ± 0.06, Ho = 0.49 ± 0.02, Ar = 4.45). In addition, we have identified signs of fragmentation in the northern habitat, which have led to significant genetic divergence between subpopulations in Russia. Multiple analyses of genetic structure support considerable genetic differentiation between the northern and western range parts, which may testify to subspecies subdivision of snow leopards from these regions. The observed patterns of genetic structure are evidence for delineation of several management units within the studied populations, requiring individual approaches for conservation initiatives, particularly related to translocation events. The causes for the revealed patterns of genetic structure and levels of genetic diversity are discussed. | ||||
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| Notes | Approved | no | |||
| Call Number | Serial | 1633 | |||
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| Author | Gosselin, S.J.; Loudy, D.L.; Tarr, M.J.; Balistreri, W.F.; Setchell, K.D.; Johnston, J.O.; Kramer, L.W.; Dresser, B.L. | ||||
| Title | Veno-occlusive disease of the liver in captive cheetah | Type | Journal Article | ||
| Year | 1988 | Publication | Vet Pathol | Abbreviated Journal | |
| Volume | 25 | Issue | 1 | Pages | 48-57 |
| Keywords | Animal; Feed; analysis; Carnivora; Cheetahs; Female; Hepatic; Veno; Occlusive; Disease; pathology; veterinary; Histocytochemistry; Liver; ultrastructure; Male; Microscopy; Electron; Support; Non-U.S.Gov't; Vitamin A; browse; non; us; gov't; government; 280 | ||||
| Abstract | Liver tissues from 126 captive cheetah were evaluated by light microscopy and histochemistry; eight animals were evaluated by electron microscopy. The main hepatic lesion, a vascular lesion resembling veno- occlusive disease (VOD) of the liver and characterized by subendothelial fibrosis and proliferation of smooth muscle-like cells in the central veins, was seen in 60% of the sexually mature cheetah. Although this hepatic vascular lesion was seen in cheetah as young as 1 year of age, the most severe lesions, usually associated with liver failure, were found in cheetah between the ages of 6 and 11. There was no sex predisposition, and in approximately 40% of the VOD cases, liver disease was not suspected clinically or at necropsy. VOD was found in other felidae, especially in the snow leopard. High levels of vitamin A in livers, as well as in diets of the cheetah, could be a contributing factor in the development of VOD in some groups of cheetah. | ||||
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| ISSN | 0300-9858 | ISBN | Medium | ||
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| Notes | Document Type: Eng | Approved | no | ||
| Call Number | SLN @ rana @ 127 | Serial | 339 | ||
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| Author | De Groot, H.; Van Swieten, P.; Aalberse, R.C. | ||||
| Title | Evidence for a Fel d I-like molecule in the “big cats” (Felidae species) | Type | Journal Article | ||
| Year | 1990 | Publication | J Allergy Clin Immunol | Abbreviated Journal | |
| Volume | 86 | Issue | 1 | Pages | 107-116 |
| Keywords | Adolescence; Adult; Allergens; immunology; Animal; Antibodies; Monoclonal; diagnostic; use; Antibody; Specificity; Carnivora; Cats; Comparative; study; Cross; Reactions; Hair; Histamine; Release; Human; IgE; analysis; IgG; Middle; Age; Radioallergosorbent; Test; methods; Support; Non-U.S.Gov't; browse; us; government; gov't; 240 | ||||
| Abstract | In this study, we investigated the cross-reactivity pattern of IgE and IgG4 antibodies to the major feline allergen, Fel d I. We studied the IgE and IgG4 response of 11 cat-allergic patients against Fel d I-like structures in eight members of the Felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. Hair from these “big cats” was collected, extracted, and used in a RAST system and histamine-release test. By means of a RAST-inhibition assay with affinity-purified Fel d I from cat dander, it was established that, in the Felidae species, a Fel d I equivalent is present that reacts with IgE and IgG4 antibodies. We found that all patients had cross-reacting IgE antibodies to seven of the Felidae tested; no IgE antibodies reactive with the caracal were found. Eight of 10 patients with IgG4 antibodies directed to cat dander also had IgG4 antibodies directed to several Felidae species, including the caracal. However, the correlation between the IgE and the IgG4 antibody specificity was low, indicating that, in the case of Fel d I IgE and IgG4, antibodies do not necessarily have the same specificity. | ||||
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| ISSN | 0091-6749 | ISBN | Medium | ||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 157 | Serial | 233 | ||
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| Author | Brown, J.L.; Wasser, S.K.; Wildt, D.E.; Graham, L.H. | ||||
| Title | Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces | Type | Journal Article | ||
| Year | 1994 | Publication | Biol Reprod | Abbreviated Journal | |
| Volume | 51 | Issue | 4 | Pages | 776-786 |
| Keywords | Animal; Carbon; Radioisotopes; Carnivora; Cats; Chromatography; High; Pressure; Liquid; Comparative Study; Estradiol; metabolism; Estrone; feces; chemistry; Female; Ovary; physiology; Pregnancy; Progesterone; Pseudopregnancy; Support; Non-U.S.Gov't; browse; non; government; gov't; us; 170 | ||||
| Abstract | Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species. | ||||
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| ISSN | 0006-3363 | ISBN | Medium | ||
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| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 251 | Serial | 198 | ||
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| Author | Janecka, J. E., Jackson, R., Munkhtsog, B., Murphy, W. J. | ||||
| Title | Characterization of 9 microsatellites and primers in snow leopards and a species-specific PCR assay for identifying noninvasive samples | Type | Journal Article | ||
| Year | 2014 | Publication | Conservation Genetic Resource | Abbreviated Journal | |
| Volume | 6 | Issue | 2 | Pages | 369:373 |
| Keywords | Microsatellites,Cytochrome b, Snow Leopard, Noninvasive genetics, Individual identification | ||||
| Abstract | Molecular markers that can effectively identify noninvasively collected samples and provide genetic information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species. |
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| Call Number | SLN @ rakhee @ | Serial | 1428 | ||
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| Author | Rodgers, T. W.,Janecka, J. E. | ||||
| Title | Applications and techniques for non-invasive faecal genetics research in felid conservation | Type | Journal Article | ||
| Year | 2013 | Publication | Abbreviated Journal | ||
| Volume | Issue | Pages | |||
| Keywords | Conservation . Elusive species . Faecal DNA . Felidae . Non-invasive genetics | ||||
| Abstract | Non-invasive genetic techniques utilising DNA extracted from faeces hold great promise for felid conservation research. These methods can be used to establish species distributions, model habitat requirements, analyse diet, estimate abundance and population density, and form the basis for population, landscape and conservation genetic analyses. Due to the elusive nature of most felid species, non-invasive genetic methods have the potential to provide valuable data that cannot be obtained with traditional observational or capture techniques. Thus, these methods are particularly valuable for research and conservation of endangered felid species. Here, we review recent studies that use non-invasive faecal genetic techniques to survey or study wild felids; provide an overview of field, laboratory and analysis techniques; and offer suggestions on how future non-invasive genetic studies can be expanded or improved to more effectively support conservation. |
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| Call Number | SLN @ rakhee @ | Serial | 1430 | ||
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| Author | Hacker, C., Atzeni, L., Munkhtsog, B., Munkhtsog, B., Galsandorj, N., Zhang, Y., Liu, Y., Buyanaa, C., Bayandonoi, G., Ochirjav, M., Farrington, J. D., Jevit, M., Zhang, Y., Wu, L. Cong, W., Li, D., Gavette, C., Jackson, R., Janecka, J. E. | ||||
| Title | Genetic diversity and spatial structures of snow leopards (Panthera uncia) reveal proxies of connectivity across Mongolia and northwestern China | Type | Journal Article | ||
| Year | 2022 | Publication | Landscape Ecology | Abbreviated Journal | |
| Volume | Issue | Pages | 1-19 | ||
| Keywords | Admixture · Central Asia · Connectivity · Habitat Modeling · Landscape Genetics · Noninvasive Genetics · MEM · Spatial Structures | ||||
| Abstract | Understanding landscape connectivity and population genetic parameters is imperative for threatened species management. However, such information is lacking for the snow leopard (Panthera uncia). This study sought to explore hierarchical snow leopard gene flow patterns and drivers of genetic structure in Mongolia and China. A total of 97 individuals from across Mongolia and from the north-eastern edge of the Qinghai-Tibetan Plateau in Gansu Province to the middle of Qinghai Province in China were genotyped across 24 microsatellite loci. Distance-based frameworks were used to determine a landscape scenario best explaining observed genetic structure. Spatial and non-spatial methods were used to investigate fine-scale autocorrelation and similarity patterns as well as genetic structure and admixture. A genetic macro-division between populations in China and Mongolia was observed, suggesting that the Gobi Desert is a substantial barrier to gene flow. However, admixture and support for a resistance-based mode of isolation suggests connective routes that could facilitate movement. Populations in Mongolia had greater connectivity, indicative of more continuous habitat. Drivers of genetic structure in China were difficult to discern, and fine-scale sampling is needed. This study elucidates snow leopard landscape connectivity and helps to prioritize conservation areas. Although contact zones may have existed and occasional crossings can occur, establishing corridors to connect these areas should not be a priority. Focus should be placed on maintaining the relatively high connectivity for snow leopard populations within Mongolia and increasing research efforts in China. | ||||
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| Call Number | SLN @ rakhee @ | Serial | 1717 | ||
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| Author | Lama, R. P., Ghale, T. R., Suwal, M. K., Ranabhat, R., Regmi, G. R. | ||||
| Title | First photographic evidence of Snow Leopard Panthera uncia (Mammalia: Carnivora: Felidae) outside current protected areas network in Nepal Himalaya | Type | Journal Article | ||
| Year | 2018 | Publication | Journal of Threatened Taxa | Abbreviated Journal | |
| Volume | Issue | Pages | 12086-12090 | ||
| Keywords | Camera-trapping, conservation, Humla, livestock depredation, monasteries, non-timber forest products, retaliatory killing, Tibetan Buddhism. | ||||
| Abstract | The Snow Leopard Panthera uncia is a rare top predator of high-altitude ecosystems and insufficiently surveyed outside of protected areas in Nepal. We conducted a rapid camera-trapping survey to assess the presence of Snow Leopard in the Limi valley of Humla District. Three individuals were recorded in two camera locations offering the first photographic evidence of this elusive cat outside the protected area network of Nepal. In addition to Snow Leopard, the Blue Sheep Pseudois nayaur, Beech Marten Martes foina, Pika Ochotona spp. and different species of birds were also detected by camera-traps. More extensive surveys and monitoring are needed for reliably estimating the population size of Snow Leopard in the area. The most urgent needs are community-based conservation activities aimed at mitigating immediate threats of poaching, retaliatory killing, and rapid prey depletion to ensure the survival of this top predator in the Himalaya. | ||||
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| Call Number | SLN @ rakhee @ | Serial | 1470 | ||
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| Author | Chetri, M, Odden, M., Sharma, K., Flagstad, O., Wegge, P | ||||
| Title | Estimating snow leopard density using fecal DNA in a large landscape in north-central Nepal | Type | Journal Article | ||
| Year | 2019 | Publication | Global Ecology and Conservation | Abbreviated Journal | |
| Volume | Issue | 17 | Pages | 1-8 | |
| Keywords | Panthera uncia, Density, Annapurna-Manaslu landscape, Noninvasive, Spatial scale | ||||
| Abstract | Although abundance estimates have a strong bearing on the conservation status of a species, less than 2% of the global snow leopard distribution range has been sampled systematically, mostly in small survey areas. In order to estimate snow leopard density across a large landscape, we collected 347 putative snow leopard scats from 246 transects (490 km) in twenty-six 5 5km sized sampling grid cells within 4393 km2 in Annapurna- Manaslu, Nepal. From 182 confirmed snow leopard scats, 81 were identified as belonging to 34 individuals; the remaining were discarded for their low (<0.625) quality index. Using maximum likelihood based spatial capture recapture analysis, we developed candidate model sets to test effects of various covariates on density and detection of scats on transects. The best models described the variation in density as a quadratic function of elevation and detection as a linear function of topography. The average density estimate of snow leopards for the area of interest within Nepal was 0.95 (SE 0.19) animals per 100 km2 (0.66e1.41 95% CL) with predicted densities varying between 0.1 and 1.9 in different parts, thus highlighting the heterogeneity in densities as a function of habitat types. Our density estimate was low compared to previous estimates from smaller study areas. Probably, estimates from some of these areas were inflated due to locally high abundances in overlap zones (hotspots) of neighboring individuals, whose territories probably range far beyond study area borders. Our results highlight the need for a large-scale approach in snow leopard monitoring, and we recommend that methodological problems related to spatial scale are taken into account in future snow leopard research. |
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| Call Number | SLN @ rakhee @ | Serial | 1478 | ||
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