Abstract: This study was undertaken in spring, 2019 to assess the applicability of the double-observer survey method for estimating blue sheep Pseudois nayaur abundance in Nar-Phu valley of Manang District located in Annapurna Conservation Area of northern Nepal. Since counting large mammals in rugged mountain habitat poses a special challenge, we tested the efficacy of the double observer method for generating robust population estimates for this important protected area. The overall detection probability for observers (O1 and O2) was 0.94 and 0.91 for a total of 106 groups comprised of 2059 individual blue sheep. We estimated the area’s blue sheep population at 2070 (SE ± 168.77; 95% CI 2059–2405) for the 246.2 km2 of sampled habitat. We determined blue sheep to be widely distributed within the study area with a mean density of 8.4 individuals per km2 based on a total study area of 246.2 km2. We discuss demographic population structure and identify limitations when applying the double observer approach, along with recommending viewshed mapping for ensuring more robust density estimates of mountain-dwelling ungulates like blue sheep or ibex that inhabit extremely heterogeneous terrain which strongly influences sighting distances and overall animal detection rates.

Abstract: Understanding landscape connectivity and population genetic parameters is imperative for threatened species management. However, such information is lacking for the snow leopard (Panthera uncia). This study sought to explore hierarchical snow leopard gene flow patterns and drivers of genetic structure in Mongolia and China. A total of 97 individuals from across Mongolia and from the north-eastern edge of the Qinghai-Tibetan Plateau in Gansu Province to the middle of Qinghai Province in China were genotyped across 24 microsatellite loci. Distance-based frameworks were used to determine a landscape scenario best explaining observed genetic structure. Spatial and non-spatial methods were used to investigate fine-scale autocorrelation and similarity patterns as well as genetic structure and admixture. A genetic macro-division between populations in China and Mongolia was observed, suggesting that the Gobi Desert is a substantial barrier to gene flow. However, admixture and support for a resistance-based mode of isolation suggests connective routes that could facilitate movement. Populations in Mongolia had greater connectivity, indicative of more continuous habitat. Drivers of genetic structure in China were difficult to discern, and fine-scale sampling is needed. This study elucidates snow leopard landscape connectivity and helps to prioritize conservation areas. Although contact zones may have existed and occasional crossings can occur, establishing corridors to connect these areas should not be a priority. Focus should be placed on maintaining the relatively high connectivity for snow leopard populations within Mongolia and increasing research efforts in China.

Abstract: The protection of Snow Leopards in the remote and economically disadvantaged Northern Areas of Pakistan needs local people equipped with the skills to gather and present information on the number and range of individual animals in their area. It is important for the success of a conservation campaign that the people living in the area are engaged in the conservation process. Snow Leopards are elusive and range through inhospitable terrain so direct study is difficult. Consequently the major goals for this project were twofold, to gather information on snow leopard distribution in this area and to train local university students and conservation management professionals in the techniques used for locating snow leopards without the need to capture or even see the animals. This project pioneered the use of DNA testing of field samples collected in Pakistan to determine the distribution of snow leopards and to attempt to identify individuals. These were collected in and around that country's most northerly national park, the Kunjurab National Park, which sits on the Pakistan China border. Though the Northern Areas is not a well developed part of Pakistan, it does possess a number of institutions that can work together to strengthen snow leopard conservation. The first of these is a newly established University with students ready to be trained in the skills needed. Secondly WWF-Pakistan has an office in the main town and a state of the art GIS laboratory in Lahore and already works closely with the Forest Department who manage the national park. All three institutions worked together in this project with WWF providing GIS expertise, the FD rangers, and the university students carrying out the laboratory work. In addition in the course of the project the University of the Punjab in Lahore also joined the effort, providing laboratory facilities for the students. As a result of this project maps have been produced showing the location of snow leopards in
two areas. Preliminary DNA evidence indicates that there is more than one animal in this
relatively small area, but the greatest achievement of this project is the training and
experience gained by the local students. For one student this has been life changing. Due to
the opportunities provided by this study the student, Nelofar gained significant scientific
training and as a consequence she is now working as a lecturer and research officer for the
Center for Integrated Mountain Research, New Campus University of the Punjab, Lahore
Pakistan