Abstract: The endangered snow leopard Panthera uncia occurs in rugged, high-altitude regions of Central Asia. However, information on the status of this felid is limited in many areas. We conducted a pilot study to optimize molecular markers for the analysis of snow leopard scat samples and to examine the feasibility of using noninvasive genetic methods for monitoring this felid. We designed snow leopard-specific primers for seven microsatellite loci that amplified shorter segments and avoided flanking sequences shared with repetitive elements. By redesigning primers we maximized genotyping success and minimized genotyping errors. In addition, we tested a Y chromosome-marker for sex identification and designed a panel of mitochondrial DNA primers for examining genetic diversity of snow leopards using scat samples. We collected scats believed to be from snow leopards in three separate geographic regions including north-western India, central China and southern Mongolia. We observed snow leopard scats in all three sites despite only brief 2-day surveys in each area. There was a high rate of species misidentification in the field with up to 54% of snow leopard scats misidentified as red fox. The high rate of field misidentification suggests sign surveys incorporating scat likely overestimate snow leopard abundance. The highest ratio of snow leopard scats was observed in Ladakh (India) and South Gobi (Mongolia), where four and five snow leopards were detected, respectively. Our findings describe a species-specific molecular panel for analysis of snow leopard scats, and highlight the efficacy of noninvasive genetic surveys for monitoring snow leopards. These methods enable large-scale noninvasive studies that will provide information critical for conservation of snow leopards.

Abstract: International collaboration to ensure the long-term survival of snow leopards (Uncia uncia) in the wild is today more acutely needed than ever! Trade in live snow leopards, their skins and bones, has during the last decade reached such extensiveness that the species is in danger of being wiped out from many of its former habitats. All recent surveys support declining populations throughout most of their range.

Abstract: Livestock depredation by the endangered snow leopard (Panthera uncia) _is an increasingly contentious issue in Himalayan villages, especially in or near protected areas. Mass attacks in which as many as 100 sheep and goats are killed in a single incident inevitably result in retaliation by local villagers. This article describes a community-based conservation initiative to address this problem in Hemis National Park, India. Human-wildlife conflict is alleviated by predator-proofing villagers' nighttime livestock pens and by enhancing household incomes in environmentally sensitive and culturally compatible ways. The authors have found that the highly participatory strategy described here (Appreciative Participatory Planning and Action-APPA) leads to a sense of project ownership by local stakeholders, communal empowerment, self-reliance, and willingness to co-exist with
snow leopards. The most significant conservation outcome of this process is the protection from retaliatory poaching of up to five snow leopards for every village's livestock pens that are made predator-proof._

Abstract: Three dog heartworms (Dirofilaria immitis) were detected in the lumen of the right cardiac ventriculus and of the pulmonary artery of a captive female snow leopard (Uncia uncia) that died of pancreatic carcinoma at a zoo in Japan. Neither clinical respiratory nor circulatory symptoms caused by the heartworm infection were observed. The filarial worms were identified as D. immitis from the morphologic characteristics of the esophagus, the presence of faint longitudinal ridges on the cuticular surface, the situation of vulva posterior to the esophagus, and the measurements of the body. The heartworms from the snow leopard were identical to that of D. immitis from dogs in the sequence of the cytochrome oxidase I region in the mitochondrial DNA. This host record is the first of D. immitis in U. uncia.

Abstract: I studied snow leopards (Panthera uncia) and blue sheep (Pseudois nayaur) in Manang District, Annapurna Conservation Area, Nepal, to estimate numbers and analyze predatorprey interactions. Five to seven adult leopards used the 105-km2 study area, a density of 4.8 to 6.7 leopards/100 km2. Density of blue sheep was 6.6-10.2 sheep/km2, and biomass density was 304 kg/km2. Estimated relative biomass consumed by snow leopards suggested that blue sheep were the most important prey; marmots (Marmota himalayana) also contributed significantly to the diet of snow leopards. Snow leopards in Manang were estimated to harvest 9-20% of total biomass and 11-24% of total number of blue sheep annually. Snow leopard :blue sheep ratio was 1 :1 14-1 :159 on a weight basis, which was considered sustainable given the importance of small mammals in the leopard's diet and the absence of other competing predators.