Abstract: A new species of Demodex is described, in all instars, from the rare and endangered snow leopard, Panthera uncia. This represents only the second demodecid from the family Felidae and the first from a wild cat species. The mite specimens were taken from juvenile hosts raised in captivity. Demodex uncii sp. nov. closely resembles Demodex cati.

Abstract: I studied snow leopards (Panthera uncia) and blue sheep (Pseudois nayaur) in Manang District, Annapurna Conservation Area, Nepal, to estimate numbers and analyze predator-prey interactions. Five to seven adult leopards used the 10-5-km-2 study area, a density of 4.8 to 6.7 leopards/100 km-2. Density of blue sheep was 6.6 10.2 sheep/km-2, and biomass density was 304 kg/km-2. Estimated relative biomass consumed by snow leopards suggested that blue sheep were the most important prey; marmots (Marmota himalayana) also contributed significantly to the diel of snow leopards Snow leopards in Manang were estimated to harvest 9-20% of total biomass and 11-24% of total number of blue sheep annually. Snow leopard: blue sheep ratio was 1:114-1:159 on a weight basis, which was considered sustainable given the importance of small mammals in the leopard's diet and the absence of other competing predators.

Abstract: Accumulation of carotenoids varies greatly among animal species and is not fully characterized.
Circulating carotenoid concentration data in captive wild animals are limited and may be useful for their management.
Serum carotenoid concentrations and dietary intakes were surveyed and the extent of accumulation
categorized for 76 species of captive wild animals at Brookfield Zoo. Blood samples were obtained opportunistically
from 275 individual animals immobilized for a variety of reasons; serum was analyzed for a- and b-carotene,
lutein 1 zeaxanthin, lycopene, b-cryptoxanthin and canthaxanthin. Total carotenoid content of diets was calculated
from tables and chemical analyses of commonly consumed dietary components. Diets were categorized as
low, moderate or high in carotenoid content as were total serum carotenoid concentrations. Animals were
classified as unknown, high, moderate or low (non-) accumulators of dietary cartenoids. Nonaccumulators had total
serum carotenoid concentrations of 0-101 nmol/L, whereas accumulators had concentrations that ranged widely,
from 225 to 35,351 nmol/L. Primates were uniquely distinguished by the widest range of type and concentration
of carotenoids in their sera. Most were classified as high to moderate accumulators. Felids had high accumulation
of b-carotene regardless of dietary intake, whereas a wide range of exotic birds accumulated only the xanthophylls,
lutein 1 zeaxanthin, canthaxanthin or cryptoxanthin. The exotic ungulates, with the exception of the bovids, had
negligible or nondetectable carotenoid serum concentrations despite moderate intakes. Bovids accumulated only
b-carotene despite moderately high lutein 1 zeaxanthin intakes. Wild captive species demonstrated a wide variety
of carotenoid accumulation patterns, which could be exploited to answer remaining questions concerning carotenoid
metabolism and function.

Abstract: Patterns of mitochondrial restriction fragment length polymorphism (RFLP) variation were used to resolve more recent relationships among the species of the Felidae ocelot lineage, domestic cat lineage, and pantherine lineage. Twenty-five of 28 restriction enzymes revealed site variation in at least 1 of 21 cat species. The ocelot lineage was resolved into three separate sister taxa groups: Geoffroy's cat (Oncifelis geoffroyi) and kodkod (O. guigna), ocelot (Leopardus pardalis) and margay (L. wiedii), and pampas cat (Lynchailurus colocolo) and most of the tigrina samples (Leopardus tigrina). Within the domestic cat lineage, domestic cat (Felis catus), European wild cat (F. silvestris), and African wild cat (F. libyca) formed a monophyletic trichotomy, which was joined with sand cat (F. margarita) to a common ancestor. Jungle cat (F. chaus) and black-footed cat (F. nigripes) mtDNAs diverged earlier than those of the other domestic cat lineage species and are less closely related. Within the pantherine lineage, phylogenetic analysis identified two distinct groups, uniting lion (P. leo) with leopard (P. pardus) and tiger (P. tigris) with snow leopard (P. uncia).

Abstract: An extraction and assay procedure to measure fecal estradiol-17-beta and progestin concentrations in several cat species was developed and validated for use for noninvasive monitoring of ovarian function. Fecal samples were collected over a range of 3-20 months from female tigers (three), lions (three), snow leopards (three), cheetahs (two), caracals (two), and domestic cats (five). Samples were extracted with 90% methanol, lipids removed with petroleum ether, and the estradiol and progestins in the methanol measured by radioimmunoassay (RIA). High Performance Liquid Chromatography (HPLC) fractionation and subsequent RIA of the fractions indicated that the estradiol-17-beta antiserum cross-reacted primarily with estradiol-17-beta in the feces of lions and tigers and was assumed to be specific for estradiol-17-beta in the feces of other species as well. However, there were several immunoreactive compounds, presumably progesterone metabolites, excreted in the feces which varied both quantitatively and qualitatively among species. The behavior of tigers, lions, cheetahs, and caracals was visually monitored during the collection period and frequency of sexual behaviors was positively correlated with increases in fecal estradiol in all species observed. The mean fecal estradiol-17-beta peaks were as follows: tigers, 128.0 +- 13.1; lions, 186.0 +- 14.8; snow leopards, 136.7 +- 15.9; cheetahs, 140.9 +- 9.0; caracals, 24.5 +- 4.0; and domestic cats 158.9 +- 19.3 ng/gm. Fecal progestin concentrations rose significantly (P lt 0,001) only after breeding or during pregnancy and were as follows: tigers, 5.6 +- 0.6; lions, 1.9 +- 0.1; cheetahs, 8.4 +- 1.1; and caracals, 2.4 +- 0.4 mu-g/gm. Fecal progestins were elevated for one-half to two-thirds of the gestation length during presumed pseudopregnancy but remained elevated throughout successful pregnancies. These results suggest that ovarian function can be monitored noninvasively in the family Felidae by the measurement of fecal estradiol-17-beta and progestin concentrations.

Abstract: This Newcastle University Expedition reviewed the conflict between man and snow leopard in the region east of Tekeli in the Dzhungarian Alatau range, Kazakhstan. After the soviet state breakup in 1991 snow leopards in Kazakhstan and in the other Asian republics were subjected to high levels of persecution. There are thought to be between 180-200 snow leopards in Kazakhstan, of which 37-40 individuals inhabit the Dzhungarian Mountains. No work has been done previously to review the present snow leopard-human conflicts in this region, and this project looked to update previous reports from Central Asia about predominant conflicts in these regions. The expedition team worked with the Institute of Zoology in Kazakhstan and two of the six person team were Kazakh students. Methods comparable to those used in previous studies were used to map and rank the threats in the western area of the range, over an eight week period. The expedition team lived in the mountains for periods of two-three weeks, carrying all their food and equipment and using local vehicles, horses and trekking to move from pasture to pasture. Semi-structured interviews, key informant interviews and prey counts were used to assess snow leopard-human conflicts including poaching of snow leopard, poaching of leopard wild prey, human disturbance and livestock overgrazing. The results from the report support much previous work from Kazakhstan and other areas in Central Asia, but serve as a useful update shedding light on an optimistic future for snow leopard conservation while highlighting what the expedition team perceives to be the major threats to snow leopards. The threats to snow leopards, in order of relevance from most to least, are loss of prey predominantly through hunting by local people (both legal and illegal), direct hunting of snowleopard for pelt/medicine, disturbance by herders on the pastures, snow leopard habitat fragmentation around the mountain bases, and least importantly overgrazing by domestic livestock. Retaliatory kills by herders are not a threat in the region and on livestock were killed by snow leopards. Levels of all hunting, legal and illegal, need to be reduced in the range until wildlife populations have recovered significantly from the mid-1990's over-hunting period. Levels of rural corruption among many stakeholders were high around the mountain region, and are thought to contribute negatively to wildlife conservation in the area. Ecotourism in the area, that incorporates local people around the mountain region, is proposed as a solution to offer local people's incentives to lower hunting levels. This report makes the following main recommendations:
ÿStrengthening law enforcement capacity
ÿIncreasing the number of ecological rangers
ÿIncreasing incentives of ecological rangers to prevent poaching
ÿEquipping the ecological rangers more sufficiently
ÿMore effective communication between ranger and National Park administrative regions
ÿThe involvement of all stakeholders in wildlife conservation including military officials and local herders
ÿWhistle blower policies to prevent illegal trade in all animals in the region
ÿMore geographic consistency between present and proposed protected areas in the region
 Accurate snow leopard monitoring to build on valuable information previously collected in Kazakhstan
 Provision of local incentives (ecotourism and community based hunting reserves) to reduce local hunting and local unemployment around the mountains
 Increased interest and technical assistance in Kazakhstan from conservation International Non-Governmental Organizations
 More government fund for the Institute of Zoology
 Stronger cross border (Kazakhstan-China) legislation implementation Further research could involve establishing an annual, standardized, snow leopard
survey in the range, or could compare these findings of threats to snow leopards to other regions in Kazakhstan (eg. The Altai) or Central Asia.
This report has been replicated for all the expedition sponsors, put on the internet, and
distributed among contacts in the snow leopard conservation community.
The expedition team also spent some time exploring and photographing some of the remoter valleys around the study pastures, and made a short video of the teams exploits. Links were made between KIMEP University in Almaty, and Newcastle University, registered a research centre with the Royal Geographical Society, and lectured about the Dzhungarian range and snow leopard conservation at; the Royal Geographical Society, Newcastle University, Royal Zoological Society for Scotland(Edinburgh Zoo), and Wilderness Lecturers (Bristol).

Abstract: Blue sheep (Pseudois nayaur) are the main prey of the endangered snow leopard (Panthera uncia) as well as an important game species in Nepal. A knowledge of how blue sheep utilize their habitat is essential for the scientific management of the sheep and for the conservation of the snow leopard, but we only have a limited understanding of this aspect of blue sheep ecology. I studied the habitat use pattern of blue sheep by direct observation in the Anna-purna Conservation Area, Nepal where they occur sympatrically with the snow leopard. The sheep used grassland habitats more frequently during pre-parturition (spring) and post-parturition (autumn) than other habitat types, but scrub and grassland habitats were used equally frequently during the rut (winter). The sheep used smooth undulating slopes of medium steepness (<40 degrees) on southerly aspects within the elevation range of 4,200-4,600 m most frequently in all seasons, and there was no evidence of seasonal migration along the elevation gradient. When not in broken landforms (e.g., cliff, landslides), the sheep maintained proximity (less than or equal to 150 m) to such features suggesting their importance as escape cover (i.e., from predators). The use of habitat components by blue sheep appeared to be related to the distribution of foraging areas and escape cover.