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Jackson, R. M., & Ahlborn, G. (1988). Observations on the Ecology of Snow Leopard in West Nepal. In H.Freeman (Ed.), (pp. 65–87). India: Snow Leopard Trust and Wildlife Institute of India.
Abstract: This summary of a four year field study by Jackson and Ahlborn begging in 1982 and concluding in 1985, discusses behaviour, trapping and tracking techniques, home range, activity patterns, prey and habitat and survey methods.
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Mallon, D. P. (1988). A Further Report on The Snow Leopard in Ladakh. In H.Freeman (Ed.), (pp. 89–97). India: Snow Leopard Trust and Wildlife Institute of India.
Abstract: A detailed knowledge of the ecology of a species is fundemental to the drawing up of effective conservation measures. One aim of the current project was to identify good areas of snow leopard habitatand evaluate them for possible inclusion in the Protected Area Network. Several good areas were surveyed and an outstanding area identified, and included in a report to the Chief Wildlife Warden.
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Fox, J. L., Sinha, S. P., Chundawat R.S., & Das, P. K. (1988). A Field Survey of Snow Leopard Presence and Habitat use in Northwestern India. In H.Freeman (Ed.), (pp. 99–111). India: International Snow Leoaprd Trust and Wildlife Institute of India.
Abstract: During November 1985 through July1996, a survey of snow leopard presence and ecology was conducted in selected areas of the states of Jammu and Kashmir, Himachal Pradesh, and Uttar Pradesh in north-western India. The study was carried out under the auspices of the Wildlife Institute of India in cooperation with the U.S. Fish and Wildlife Service and the International Snow Leopard Trust. The objectives of the survey were essentially determine the relative presence of the snow leopard and its associated prey species,investigate human interaction with the snow leopard and select an appropriate site for more intensive studies of the snow leopard and its ecosystem.
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Hast, M. H. (1989). The larynx of roaring and non-roaring cats. J Anat, 163, 117–121.
Abstract: Dissections were made of the larynges of 14 species of the cat family, with representative specimens from all genera. It was found that the vocal folds of the larynx of genus Panthera (with the exception of the snow leopard) form the basic structure of a sound generator well- designed to produce a high acoustical energy. Combined with an efficient sound radiator (vocal tract) that can be adjusted in length, a Panthera can use its vocal instrument literally to blow its own horn with a 'roar'. Also, it is proposed that laryngeal morphology can be used as an anatomical character in mammalian taxonomy.
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De Groot, H., Van Swieten, P., & Aalberse, R. C. (1990). Evidence for a Fel d I-like molecule in the “big cats” (Felidae species). J Allergy Clin Immunol, 86(1), 107–116.
Abstract: In this study, we investigated the cross-reactivity pattern of IgE and IgG4 antibodies to the major feline allergen, Fel d I. We studied the IgE and IgG4 response of 11 cat-allergic patients against Fel d I-like structures in eight members of the Felidae family: ocelot, puma, serval, siberian tiger, lion, jaguar, snow leopard, and caracal. Hair from these “big cats” was collected, extracted, and used in a RAST system and histamine-release test. By means of a RAST-inhibition assay with affinity-purified Fel d I from cat dander, it was established that, in the Felidae species, a Fel d I equivalent is present that reacts with IgE and IgG4 antibodies. We found that all patients had cross-reacting IgE antibodies to seven of the Felidae tested; no IgE antibodies reactive with the caracal were found. Eight of 10 patients with IgG4 antibodies directed to cat dander also had IgG4 antibodies directed to several Felidae species, including the caracal. However, the correlation between the IgE and the IgG4 antibody specificity was low, indicating that, in the case of Fel d I IgE and IgG4, antibodies do not necessarily have the same specificity.
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Hochstrasser, K., Wachter, E., Reisinger, P. W., Greim, M., Albrecht, G. J., & Gebhard, W. (1993). Amino acid sequences of mammalian kazal-type proteinase inhibitors from salivary glands. Comp Biochem Physiol B, 106(1), 103–108.
Abstract: 1. The amino acid sequences of bikazins (the double-headed Kazal-type proteinase inhibitors from submandibular glands) isolated from the snow leopard (Unica unica), the European mink (Mustela lutreola), and the European pine marten (Martes martes) were determined. 2. N-terminal domains of bikazins are characterized by a cysteine residue spacing that differs from that of C-terminal domains of bikazins and other Kazal-type proteinase inhibitor domains. 3. N-terminal sequences of bikazins seem to be specific for, and highly conserved within, each Carnivora family.
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Ferguson, D. A. (1997). International Cooperation for Snow Leopard and Biodiversity Conservation: The Government Perspective. In R.Jackson, & A.Ahmad (Eds.), (pp. 178–193). Lahore, Pakistan: Islt.
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Jackson, R., Zongyi, W., Xuedong, L., & Yun, C. (1994). Snow Leopards in the Qomolangma Nature Preserve of Tibet Autonomous Region. In J.L.Fox, & D.Jizeng (Eds.), (pp. 85–95). Usa: Islt.
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Brown, J. L., Wasser, S. K., Wildt, D. E., & Graham, L. H. (1994). Comparative Aspects of Steroid Hormone Metabolism and Ovarian Activity in Felids, Measured Noninvasively in Feces. Biol Reprod, 51(4), 776–786.
Abstract: Noninvasive fecal assays were used to study steroid metabolism and ovarian activity in several felid species. Using the domestic cat (Felis catus) as model, the excretory products of injected [14C]estradiol (E2) and [14C]progesterone (P4) were determined. Within 2 days, 97.0 +/- 0.6% and 96.7 +/- 0.5% of recovered E2 and P4 radioactivity, respectively, was found in feces. E2 was excreted as unconjugated estradiol and estrone (40%) and as a non-enzyme- hydrolyzable conjugate (60%). P4 was excreted primarily as non-enzyme- hydrolyzable, conjugated metabolites (78%) and as unconjugated pregnenolone epimers. A simple method for extracting fecal steroid metabolites optimized extraction efficiencies of the E2 and P4 excretion products (90.1 +/- 0.8% and 87.2 +/- 1.4%, respectively). Analysis of HPLC fractions of extracted fecal samples from the radiolabel-injected domestic cats revealed that E2 immunoreactivity coincided primarily with the unconjugated metabolized [14C]E2 peak, whereas progestogen immunoreactivity coincided with a single conjugated epimer and multiple unconjugated pregnenolone epimers. After HPLC separation, similar immunoreactive E2 and P4 metabolite profiles were observed in the leopard cat (F. bengalensis), cheetah (Acinonyx jubatus), clouded leopard (Neofelis nebulosa), and snow leopard (Panthera uncia). Longitudinal analyses demonstrated that changes in fecal E2 and P4 metabolite concentrations reflected natural or artificially induced ovarian activity. For example, severalfold increases in E2 excretion were associated with overt estrus or exogenous gonadotropin treatment, and elevated fecal P4 metabolite concentrations occurred during pregnant and nonpregnant (pseudopregnant) luteal phases. Although overall concentrations were similar, the duration of elevated fecal P4 metabolites during pseudopregnancy was approximately half that observed during pregnancy. In summary, steroid metabolism mechanisms appear to be conserved among these physically diverse, taxonomically related species. Results indicate that this hormone-monitoring approach will be extremely useful for elucidating the hormonal regulatory mechanism associated with the reproductive cycle, pregnancy, and parturition of intractable and endangered felid species.
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Johnston, L. A., Armstrong, D. L., & Brown, J. L. (1994). Seasonal effects on seminal and endocrine traits in the captive snow leopard (Panthera uncia). J Reprod Fertil, 102(1), 229–236.
Abstract: The annual reproductive cycle of the male snow leopard (Panthera uncia) was characterized by evaluating seminal and endocrine traits monthly. Testicular volume was greatest (P < 0.05) during the winter months when the quality of ejaculate was optimal. Ejaculate volume, total sperm concentration ml-1, motile sperm concentration per ejaculate, sperm morphology and sperm motility index were lowest during the summer and autumn months compared with the winter and spring. Peripheral LH, FSH and testosterone concentrations were also lowest during the summer months, increasing during the autumn just before the increase in semen quality, and were maximal during the winter months. There was a direct relationship (P < 0.01) between: (1) testosterone and testicular volume, total sperm concentration ml-1, motile sperm concentration per ejaculate and ejaculate volume, and (2) LH and testicular volume and motile sperm concentration per ejaculate. In summary, although spermatozoa were recovered throughout the year, optimal gamete quality was observed during the winter and spring. Although previous studies in felids have demonstrated seasonal effects on either seminal or endocrine traits, this is the first study to demonstrate a distinct effect of season on both pituitary and testicular function.
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