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Author  |
Roth, T.L.; Howard, J.G.; Donoghue, A.M.; Swanson, W.F.; Wildt, D.E. | ||||
| Title | Function and culture requirements of snow leopard (Panthera uncia) spermatozoa in vitro | Type | Journal Article | ||
| Year | 1994 | Publication | J Reprod Fertil | Abbreviated Journal | |
| Volume | 101 | Issue | 3 | Pages | 563-569 |
| Keywords | Animal; Carnivora; physiology; Cell; survival; Cells; Cultured; Comparative; study; Culture; Media; Female; Male; Pentoxifylline; Sperm; Motility; Ovum; Interactions; Spermatozoa; cytology; Support; Non-U.S.Gov't; U.S.Gov't; P.H.S.; browse; non; us; gov't; government; 190 | ||||
| Abstract | Electroejaculates from eight snow leopards were used to determine how the motility of spermatozoa was influenced by (i) type of media (Ham's F10, PBS, human tubal fluid or RPMI-1640); (ii) holding temperature (23 degrees C versus 37 degrees C); (iii) washing of spermatozoa and (iv) a sperm metabolic enhancer, pentoxifylline. The duration of sperm motility was assessed by evaluating samples in each treatment every hour for 6 h and a sperm motility index (a value combining percentage sperm motility and rate of forward progression) calculated. Spermatozoa from the Ham's F10, PBS and PBS plus pentoxifylline treatments were also co-incubated with zona-intact, domestic cat eggs that were fixed and evaluated for spermatozoa bound to the zona pellucida, penetrating the outer and inner layers of the zona pellucida and within the perivitelline space. During the 6 h co-incubation, the sperm motility index in PBS with pentoxifylline was greater (P < 0.05) than in PBS alone which, in turn, was greater (P < 0.05) than in the other three test media. Washing the spermatozoa enhanced (P < 0.05) motility in both PBS and PBS plus pentoxifylline relative to unwashed samples, but there was no effect (P > 0.05) of holding temperature. Pentoxifylline supplementation enhanced (P < 0.05) the proportion of cat eggs with bound, but not penetrated, snow leopard spermatozoa in the inner layer of the zona pellucida, and there were no spermatozoa in the perivitelline space.(ABSTRACT TRUNCATED AT 250 WORDS) | ||||
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| ISSN | 0022-4251 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 253 | Serial | 828 | ||
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| Author |
Roth, T.L.; Armstrong, D.L.; Barrie, M.T.; Wildt, D.E. | ||||
| Title | Seasonal effects on ovarian responsiveness to exogenous gonadotrophins and successful artificial insemination in the snow leopard (Uncia uncia) | Type | Journal Article | ||
| Year | 1997 | Publication | Reprod Fertil Dev | Abbreviated Journal | |
| Volume | 9 | Issue | 3 | Pages | 285-295 |
| Keywords | Animal; Carnivora; anatomy; histology; Blood; physiology; Estradiol; Female; Gonadotropins; administration; dosage; pharmacology; Chorionic; Equine; Human; Insemination; artificial; methods; veterinary; Laparoscopy; Male; Oocytes; cytology; Ovary; drug; effects; Ovulation; Induction; Pregnancy; Progesterone; Seasons; Support; Non-U.S.Gov't; browse; non; us; gov't; government; 400 | ||||
| Abstract | Ovaries of the seasonally-breeding snow leopard (Uncia uncia) were examined to determine whether they were responsive to exogenous gonadotrophins throughout the year. The potential of laparoscopic artificial insemination (AI) also was assessed for producing offspring. During the non-breeding, pre-breeding, breeding and post-breeding seasons, females (n = 20) were treated with a standardized, dual- hormone regimen given intramuscularly (600 I.U. of equine chorionic gonadotrophin followed 80-84 h later with 300 I.U. of human chorionic gonadotrophin (hCG)). Laparoscopy was performed 45-50 h after administration of hCG, and all ovarian structures were described. Females with fresh corpora lutea (CL) were inseminated, and anovulatory females were subjected to follicular aspiration to examine oocyte quality. Snow leopards responded to exogenous gonadotrophins throughout the year. Mean number of total ovarian structures (distinct follicles mature in appearance plus CL) did not differ (P > or = 0.05) with season, but the proportion of CL: total ovarian structures was greater (P < 0.01) for the breeding season compared with all other seasons. The proportion of females ovulating was greater (P < 0.05) during the breeding and post-breeding seasons than during the pre-breeding and non- breeding seasons respectively. No Grade-1 quality oocytes were recovered from follicles of anovulatory females. Serum concentrations of oestradiol-17 beta appeared elevated in all females, and neither oestradiol-17 beta concentrations nor progesterone concentrations differed (P > or = 0.05) among seasons. Of 15 females artificially inseminated, the only one that was inseminated in the non-breeding season became pregnant and delivered a single cub. This is the first successful pregnancy resulting from AI in this endangered species. | ||||
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| ISSN | 1031-3613 | ISBN | Medium | ||
| Area | Expedition | Conference | |||
| Notes | Document Type: eng | Approved | no | ||
| Call Number | SLN @ rana @ 325 | Serial | 830 | ||
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| Author |
Rosenthal, M.; Ott, J.L. | ||||
| Title | Snow Leopard Captive Mangement Survey | Type | Book Whole | ||
| Year | 1988 | Publication | Abbreviated Journal | ||
| Volume | Issue | Pages | |||
| Keywords | captivity; zoos; zoo; management; browse; 3680 | ||||
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| Publisher | Lincon Park Zoological Gardens | Place of Publication | Chicago | Editor | |
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| Notes | Approved | no | |||
| Call Number | SLN @ rana @ 126 | Serial | 827 | ||
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| Author |
Rosen, T. Hussain, S. Mohammad, G. Jackson, R. Janecka, J, E. Michel, S. | ||||
| Title | Reconciling Sustainable Development of Mountain Communities With Large Carnivore Conservation | Type | Journal Article | ||
| Year | 2012 | Publication | Mountain Research and Development | Abbreviated Journal | |
| Volume | Issue | 32(3) | Pages | 286-293 | |
| Keywords | Carnivores; conflict; conservation; incentives; livestock; insurance scheme; community empowerment; Pakistan | ||||
| Abstract | While the world is becoming increasingly interconnected and interdependent, physically and culturally, the wildlife of remote mountain regions is being affected both positively and negatively by such interconnectedness. In the case of snow leopards, the conservation impact has been largely, and rather unexpectedly, positive: Species-focused conservation projects, such as Project Snow Leopard (PSL) in Gilgit-Baltistan, remain mainly externally driven initiatives. PSL, initiated as a small pilot project in 1998, has relied on an approach that includes the use of an insurance scheme, the deployment of mitigation measures, and the empowerment of local governance. This approach has been successful in reducing the conflict with snow leopards and has built greater tolerance toward them. PSL is managed by local communities and cofinanced by them. PSL communities throughout the region are bearing the burden of carnivore conservation, and they are unwittingly subsidizing their populations by ‘‘feeding’’ them their livestock even though they are an economic threat to them. In this article, we argue that external intervention in the form of efforts that help alleviate the consequences of conflict through local empowerment have had a positive impact on the local mountain societies. We also show that such interventions have resulted in tangible conservation results, with the number of snow leopards staying at least stable. Our experience also shows that while the incentive component is critical, it is also part of a larger approach—one that includes developing and supporting local governance structures, improving access to education, and offering a range of tools to reduce the conflict that can be implemented locally. Finally, we suggest that investing in this approach— one that recognizes the species and local-context complexities surrounding the implementation of conservation incentives—can continue to inform international practices and guidelines for reducing human–wildlife conflicts worldwide. |
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| Notes | Approved | no | |||
| Call Number | SLN @ rakhee @ | Serial | 1387 | ||
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| Author |
Rosen, T. | ||||
| Title | From Yellowstone to the Karakorums: A journey to understand conflicts with large carnivores | Type | Magazine Article | ||
| Year | 2010 | Publication | NRCC News | Abbreviated Journal | |
| Volume | 23 | Issue | 1 | Pages | 12-13 |
| Keywords | Pakistan, Project Snow Leopard, Baltistan Wildlife Conservation and Development Organization | ||||
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| Language | English | Summary Language | Original Title | ||
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| Notes | NRCC News (Northern Rockies Conservation Cooperative) annual newsletter: bridging science and policy to advance conservation. Fall 2010, issue 23(1). | Approved | no | ||
| Call Number | SLN @ rana @ | Serial | 1295 | ||
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| Author |
Rodgers, T. W.,Janecka, J. E. | ||||
| Title | Applications and techniques for non-invasive faecal genetics research in felid conservation | Type | Journal Article | ||
| Year | 2013 | Publication | Abbreviated Journal | ||
| Volume | Issue | Pages | |||
| Keywords | Conservation . Elusive species . Faecal DNA . Felidae . Non-invasive genetics | ||||
| Abstract | Non-invasive genetic techniques utilising DNA extracted from faeces hold great promise for felid conservation research. These methods can be used to establish species distributions, model habitat requirements, analyse diet, estimate abundance and population density, and form the basis for population, landscape and conservation genetic analyses. Due to the elusive nature of most felid species, non-invasive genetic methods have the potential to provide valuable data that cannot be obtained with traditional observational or capture techniques. Thus, these methods are particularly valuable for research and conservation of endangered felid species. Here, we review recent studies that use non-invasive faecal genetic techniques to survey or study wild felids; provide an overview of field, laboratory and analysis techniques; and offer suggestions on how future non-invasive genetic studies can be expanded or improved to more effectively support conservation. |
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| Notes | Approved | no | |||
| Call Number | SLN @ rakhee @ | Serial | 1430 | ||
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| Author |
Rodenburg, W.F. | ||||
| Title | The Trade in Wild Animal Furs in Afghanistan | Type | Miscellaneous | ||
| Year | 1977 | Publication | Abbreviated Journal | ||
| Volume | Issue | Pages | |||
| Keywords | Afghanistan; pelts; hunting; poaching; pelt; coat; fur; skin; browse; 2480 | ||||
| Abstract | Estimated that 70-80 snow leopard pelts were traded annually at the Kabul bazaar. | ||||
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| Notes | UNDP/FAO Project Field Document AFG/74/016, Kabul. Date of Copyright: 1977 | Approved | no | ||
| Call Number | SLN @ rana @ 40 | Serial | 826 | ||
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| Author |
Rode, J., Pelletier, A., Fumey, J., Rode, S., Cabanat, A. L., Ouvrard, A., Chaix, B., White, B., Harnden, M., Xuan, N. T., Vereshagin, A., Casane, D. | ||||
| Title | Diachronic monitoring of snow leopards at Sarychat-Ertash State Reserve (Kyrgyzstan) through scat genotyping: a pilot study | Type | Journal Article | ||
| Year | 2020 | Publication | bioRxiv | Abbreviated Journal | |
| Volume | Issue | Pages | 1-21 | ||
| Keywords | snow leopard, noninvasive genotyping, population dynamics, microsatellite, relatedness, diachronic monitoring, citizen science, Central Asia | ||||
| Abstract | Snow leopards (Panthera uncia) are a keystone species of Central Asia’s high mountain ecosystem. The species is listed as vulnerable and is elusive, preventing accurate population assessments that could inform conservation actions. Non-invasive genetic monitoring conducted by citizen scientists offers avenues to provide key data on this species that would otherwise be inaccessible. From 2011 to 2015, OSI-Panthera citizen science expeditions tracked signs of presence of snow leopards along transects in the main valleys and crests of the Sarychat-Ertash State Reserve (Kyrgyzstan). Scat samples were genotyped at seven autosomal microsatellite loci and at a X/Y locus for sex identification, which allowed estimating a minimum of 11 individuals present in the reserve from 2011 to 2015. The genetic recapture of 7 of these individuals enabled diachronic monitoring, providing indications of individuals’ movements throughout the reserve. We found putative family relationships between several individuals. Our results demonstrate the potential of this citizen science program to get a precise description of a snow leopard population through time. | ||||
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| Notes | Approved | no | |||
| Call Number | Serial | 1602 | |||
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| Author |
Rode, J., Lambert, C., Marescot, L., Chaix, B., Beesau, J., Bastian, S., Kyrbashev, J., Cabanat, A.L. | ||||
| Title | Population monitoring of snow leopards using camera trapping in Naryn State Nature Reserve, Kyrgyzstan, between 2016 and 2019 | Type | Journal Article | ||
| Year | 2021 | Publication | Global Ecology and Conservation | Abbreviated Journal | |
| Volume | 31 | Issue | e01850 | Pages | 1-6 |
| Keywords | Snow leopard, camera trapping, Citizen science, Kyrgyzstan | ||||
| Abstract | Four field seasons of snow leopard (Panthera uncia) camera trapping inside Naryn State Nature Reserve, Kyrgyzstan, performed thanks to citizen science expeditions, allowed detecting a minimal population of five adults, caught every year with an equilibrated sex ratio (1.5:1) and reproduction: five cubs or subadults have been identified from three litters of two different females. Crossings were observed one to three times a year, in front of most camera traps, and several times a month in front of one of them. Overlap of adults’ minimal territories was observed in front of several camera traps, regardless of their sex. Significant snow leopard presence was detected in the buffer area and at Ulan area which is situated at the reserve border. To avoid poaching on this apex predator and its preys, extending the more stringent protection measures of the core zone to both the Southern buffer area and land adjacent to Ulan is recommended. | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rakhee @ | Serial | 1660 | ||
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| Author |
Robinson, J. J., Crichlow, A. D., Hacker, C. E., Munkhtsog, B., Munkhtsog, B., Zhang, Y., Swanson, W. F., Lyons, L. A., Janecka, J. E. | ||||
| Title | Genetic Variation in the Pallas’s Cat (Otocolobus manul) in Zoo-Managed and Wild Populations | Type | Journal Article | ||
| Year | 2024 | Publication | Diversity | Abbreviated Journal | |
| Volume | 16 | Issue | 228 | Pages | 1-13 |
| Keywords | Otocolobus manul; microsatellites; zoo-managed population; mitochondrial 12S ribosomal RNA; endothelial PAS domain protein 1 | ||||
| Abstract | The Pallas’s cat (Otocolobus manul) is one of the most understudied taxa in the Felidae family. The species is currently assessed as being of “Least Concern” in the IUCN Red List, but this assessment is based on incomplete data. Additional ecological and genetic information is necessary for the long-term in situ and ex situ conservation of this species. We identified 29 microsatellite loci with sufficient diversity to enable studies into the individual identification, population structure, and phylogeography of Pallas’s cats. These microsatellites were genotyped on six wild Pallas’s cats from the Tibet Autonomous Region and Mongolia and ten cats from a United States zoo-managed population that originated in Russia and Mongolia. Additionally, we examined diversity in a 91 bp segment of the mitochondrial 12S ribosomal RNA (MT-RNR1) locus and a hypoxia-related gene, endothelial PAS domain protein 1 (EPAS1). Based on the microsatellite and MT-RNR1 loci, we established that the Pallas’s cat displays moderate genetic diversity. Intriguingly, we found that the Pallas’s cats had one unique nonsynonymous substitution in EPAS1 not present in snow leopards (Panthera uncia) or domestic cats (Felis catus). The analysis of the zoo-managed population indicated reduced genetic diversity compared to wild individuals. The genetic information from this study is a valuable resource for future research into and the conservation of the Pallas’s cat. | ||||
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| Notes | Approved | no | |||
| Call Number | SLN @ rakhee @ | Serial | 1749 | ||
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