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	Author	Zhang, F.; Jiang, Z.; Zeng, Y.; McCarthy, T.
	Title	Development of primers to characterize the mitochondrial control region of the snow leopard (Uncia uncia)			Type	Miscellaneous
	Year	2007	Publication	Molecular Ecology Notes	Abbreviated Journal
	Volume	7	Issue		Pages	1196-1198
	Keywords	control region,Felidae,mitochondrial DNA,snow leopard,species-specific primers; genetics; development; mitochondrial; control; region; snow; snow leopard; snow-leopard; leopard; uncia
	Abstract	The snow leopard (Uncia uncia) is a rare carnivore living above the snow line in central Asia. Using universal primers for the mitochondrial genome control region hypervariable region 1 (HVR1), we isolated a 411-bp fragment of HVR1 and then designed specific primers near each end of this sequence in the conserved regions. These primers were shown to yield good polymerase chain reaction products and to be species specific. Of the 12 snow leopards studied, there were 11 segregating sites and six haplotypes. An identification case of snow leopard carcass (confiscated by the police) proved the primers to be a useful tool for forensic diagnosis in field and population genetics studies.
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	Notes				Approved	no
	Call Number	SLN @ rana @ 911			Serial	1073
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	Author	Wildt, D.; Pukazhenthi, B.; Brown, J.; Monfort, S.; Howard, J.; Roth, T.
	Title	Spermatology for understanding, managing and conserving rare species			Type	Journal Article
	Year	1995	Publication	Reproduction Fertility and Development	Abbreviated Journal
	Volume	7	Issue	4	Pages	811-824
	Keywords	Assisted-Reproduction; Cryopreservation; reproduction; zoo; medical; veterinary; spermatology; genetics; browse; assisted; 1380
	Abstract	Most conventional spermatology research involves common mammalian species including livestock, laboratory animals and humans. Yet, there are more than 4500 mammalian species inhabiting the planet for which little is known about basic reproductive biology, including sperm characteristics and function. This information is important, not just as adjunct knowledge, but because the majority of these species are threatened with extinction, largely due to human-induced pressures. The field of conservation is changing rapidly, and global cooperation is emerging among a variety of wildlife enthusiasts, ranging from management authorities of nature reserves to curators of rare zoological collections. Conservation progress depends on systematic, multidisciplinary research first to answer basic questions, with new data then applied to endangered species management plans. The reproductive physiologist is a crucial component of this scheme. Reproduction is the essence of species survival, and enormous effort needs to be directed at these 'untraditional' research species, subspecies and populations. Spermatology research combined with simultaneous efforts in endocrinology, embryology and cryopreservation (among others) can lead to the successful application of assisted reproduction. Examples from this laboratory include an array of wild felid species and a rare cervid and mustelid. Obstacles to success are formidable, including unique species-specificities, diminished genetic diversity and a general lack of resources. Nonetheless, the field offers tremendous opportunities for generating unique knowledge of comparative interest and with conservation utility.
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	Notes	Document Type: English			Approved	no
	Call Number	SLN @ rana @ 278			Serial	1024
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	Author	Wharton, D.; Freeman, H.
	Title	The Snow Leopard in North America: Captive Breeding Under the Species Survival PLan			Type	Conference Article
	Year	1988	Publication		Abbreviated Journal
	Volume		Issue		Pages	131-136
	Keywords	Species-Survival-Plan; captivity; breeding; genetics; zoos; studs; captive; browse; species; survival; plan; 1720
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	Publisher	International Snow Leoaprd Trust and WIldlife Institute of India	Place of Publication	India	Editor	H.Freeman
	Language		Summary Language		Original Title
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	Notes	Full Text at URLTitle, Monographic: Fifth International Snow Leopard SymposiumPlace of Meeting: Srinagar, IndiaDate of Copyright: 1988			Approved	no
	Call Number	SLN @ rana @ 134			Serial	1016
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	Author	Wasser, S.
	Title	Snow Leopard Genetics: New Techniques			Type	Miscellaneous
	Year	1998	Publication		Abbreviated Journal
	Volume	xvi	Issue		Pages	8
	Keywords	genetics; medicine; veterinary; Dna; feces; scat; browse; 4500
	Abstract
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	Publisher	Islt	Place of Publication	Seattle	Editor
	Language		Summary Language		Original Title
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	Notes	Full Text at URLJournal Title: Snow Line			Approved	no
	Call Number	SLN @ rana @ 438			Serial	1009
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	Author	Warren E.Johnson, E.E.
	Title	The Late Miocene Radiation of Modern Felidae: A Genetic Assessment			Type	Miscellaneous
	Year	2006	Publication	Science	Abbreviated Journal
	Volume	311	Issue		Pages	73-77
	Keywords	classification; divergence; Dna; Felidae; genetics; Miocene; mitochondrial; phylogeny; radiation; species; taxonomic
	Abstract	Modern felid species descend from relatively recent (<11 million years ago) divergence and speciation events that produced successful predatory carnivores worldwide but that have confounded taxonomic classifications. A highly resolved molecular phylogeny with divergence dates for all living cat species, derived from autosomal, X-linked, Y-linked, and mitochondrial gene segments (22,789 base pairs) and 16 fossil calibrations define eight principal lineages produced through at least 10 intercontinental migrations facilitated by sea-level fluctuations. A ghost lineage analysis indicates that available felid fossils underestimate (i.e., unrepresented basal branch length) first occurrence by an average of 76%, revealing a low representation of felid lineages in paleontological remains. The phylogenetic performance of distinct gene classes showed that Y-chromosome segments are appreciably more informative than mitochondrial DNA, X-linked, or autosomal genes in resolving the rapid Felidae species radiation.
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	Publisher		Place of Publication	Washington D.C.	Editor	Jill Pecon-Slattery, W.J.M., Agostinho Antunes, Emma Teeling, Stephen J.O'Brien
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	Notes				Approved	no
	Call Number	SLN @ rana @ 880			Serial	1008
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	Author	Trepanier, L.A.; Cribb, A.E.; Spielberg, S.P.; Ray, K.
	Title	Deficiency of cytosolic arylamine N-acetylation in the domestic cat and wild felids caused by the presence of a single NAT1-like gene			Type	Journal Article
	Year	1998	Publication	Pharmacogenetics	Abbreviated Journal
	Volume	8	Issue	2	Pages	169-179
	Keywords	Acetylation; Amino; Acid; Sequence; Animal; Arylamine; N-Acetyltransferase; metabolism; Base; Blotting; Southern; Carnivora; genetics; Cats; Cytosol; enzymology; Dna; Human; Isoenzymes; Liver; Molecular; Data; Polymerase; Chain; Reaction; Rabbits; Homology; Nucleic Acid; Substrate; Specificity; Support; U.S.Gov't; P.H.S.; browse; nucleic; us; government; 130
	Abstract	The purpose of this study was to determine the molecular basis for a relative deficiency in the cat of cytosolic arylamine N- acetyltransferase (NAT), an enzyme family that is important in the metabolism of xenobiotics and that normally consists of at least two related enzymes, NAT1 and NAT2. N-acetyltransferase in feline liver showed high affinity (mean Km = 2.1 microM) for p-aminobenzoic acid, an NAT1 selective substrate in humans and rabbits, but showed a very poor affinity (mean Km > 10 mM) for sulfamethazine, an NAT2 selective substrate in humans and rabbits. Immunoreactive N-acetyltransferase was detected in feline liver, bladder and colon using an NAT1-specific antipeptide antibody, but was not detected in any tissues using an NAT2- specific antibody. Southern blot analysis of genomic DNA demonstrated a single band in domestic cats using each of six restriction digests; single bands were also found on Southern blot analysis of six wild felids. The deduced amino acid sequence of the central portion of feline N-acetyltransferase, obtained by polymerase chain reaction amplification in both domestic cats and seven wild felids (lion, tiger, lynx, snow leopard, bobcat, Asian leopard cat and cheetah), contained three residues, Phe125, Arg127, and Tyr129, which determine NAT1-like substrate specificity in humans. These results support the conclusion that cytosolic arylamine N-acetylation activity is low in the cat because of the presence of a single N-acetyltransferase that has substrate specificity, immunogenicity and sequence characteristics similar to human NAT1, and that the unusual presence of only a single N- acetyltransferase gene appears to be a family wide trait shared by other felids.
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	Publisher		Place of Publication		Editor
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	Series Volume		Series Issue		Edition
	ISSN	0960-314x	ISBN		Medium
	Area		Expedition		Conference
	Notes	Document Type: eng			Approved	no
	Call Number	SLN @ rana @ 345			Serial	968
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	Author	Roth, T.L.; Swanson, W.F.; Wildt, D.E.
	Title	Snow leopard (Panthera unica) sperm longevity in vitro is not influenced by protein or energy source supplements but is affected by buffer source			Type	Journal Article
	Year	1995	Publication	Theriogenology	Abbreviated Journal
	Volume	43	Issue	1	Pages	309
	Keywords	genetics; Sperm; captivity; medical; medicine; veterinary; zoo; breeding; browse; 1400
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	Notes	Annual Conference of the International Embryo Transfer Society, Calgary, Alberta, Canada, January 8-10, 1995 Document Type: English Call Number: QP251 .T44			Approved	no
	Call Number	SLN @ rana @ 280			Serial	829
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	Author	Rodgers, T. W.,Janecka, J. E.
	Title	Applications and techniques for non-invasive faecal genetics research in felid conservation			Type	Journal Article
	Year	2013	Publication		Abbreviated Journal
	Volume		Issue		Pages
	Keywords	Conservation . Elusive species . Faecal DNA . Felidae . Non-invasive genetics
	Abstract	Non-invasive genetic techniques utilising DNA extracted from faeces hold great promise for felid conservation research. These methods can be used to establish species distributions, model habitat requirements, analyse diet, estimate abundance and population density, and form the basis for population, landscape and conservation genetic analyses. Due to the elusive nature of most felid species, non-invasive genetic methods have the potential to provide valuable data that cannot be obtained with traditional observational or capture techniques. Thus, these methods are particularly valuable for research and conservation of endangered felid species. Here, we review recent studies that use non-invasive faecal genetic techniques to survey or study wild felids; provide an overview of field, laboratory and analysis techniques; and offer suggestions on how future non-invasive genetic studies can be expanded or improved to more effectively support conservation.
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	Notes				Approved	no
	Call Number	SLN @ rakhee @			Serial	1430
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	Author	McCarthy, T.; Murray, K.; Sharma, K.; Johansson, O.
	Title	Preliminary results of a long-term study of snow leopards in South Gobi, Mongolia			Type	Journal Article
	Year	2010	Publication	Cat News	Abbreviated Journal
	Volume	Autumn	Issue	53	Pages	15-19
	Keywords	snow leopard, Mongolia, monitor, population, Panthera, Snow Leopard Trust, Snow Leopard Conservation Fund, South Gobi, ecology, radio collar, GPS-satellite collar, home range, camera trapping, fecal genetics, occupancy modeling
	Abstract	Snow leopards Panthera uncia are under threat across their range and require urgent conservation actions based on sound science. However, their remote habitat and cryptic nature make them inherently difficult to study and past attempts have provided insufficient information upon which to base effective conservation. Further, there has been no statistically-reliable and cost-effective method available to monitor snow leopard populations, focus conservation effort on key populations, or assess conservation impacts. To address these multiple information needs, Panthera, Snow Leopard Trust, and Snow Leopard Conservation Fund, launched an ambitious long-term study in Mongolia’s South Gobi province in 2008. To date, 10 snow leo-pards have been fitted with GPS-satellite collars to provide information on basic snow leopard ecology. Using 2,443 locations we calculated MCP home ranges of 150 – 938 km2, with substantial overlap between individuals. Exploratory movements outside typical snow leopard habitat have been observed. Trials of camera trapping, fecal genetics, and occupancy modeling, have been completed. Each method ex-hibits promise, and limitations, as potential monitoring tools for this elusive species.
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	Publisher		Place of Publication		Editor
	Language	English	Summary Language		Original Title
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	Notes				Approved	no
	Call Number	SLN @ rana @			Serial	1151
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	Author	Manati, A.R.
	Title	Fur trade of large cats and the question of the subspecies status of leopards in Afghanistan (Der Handel mit Fellen von Grosskatzen und die Abklärung der Unterartenfrage beim			Type	Book Whole
	Year	2008	Publication		Abbreviated Journal
	Volume		Issue		Pages	125
	Keywords	fur trade, genetics, leopard, Panthera pardus, snow leopard, subspecies, Uncia uncia
	Abstract	Over a time of four years the bazars of Afghanistan were surveyed for furs of spotted wild cats, in particular leopards and snow leopards. In 2004 in Kabul a total of 28 furs of leopards were purchased by shopkeepers and 21 sold at an average price of 825 $. In the same year 25 furs of snow leopards were purchased and 19 sold to clients at an average price of 583 $. In 2006 at a single inspection double as many furs of leopards were found to be offered for sale in comparison to the whole year of 2004. Also prices had increased over the two years by 20 % to an average of 1037 $. Similarly the number of furs of snow leopards at 21 pieces was higher than in 2004, and the prices had increased to an average of 652 $. In 2007 investigations rendered more difficult, because the authorities had started to control the fur trade, and the results are not unequivocal. Clients were without any exception foreigners. Surveys in 2004 in Mazar-e-Sharif, Kunduz, Takhar and Faiz Abad, in 2006 additionally in Baharak and Iskashem in the province of Badakhshan, revealed a regular trade in furs of spotted cats, however not as extensive as in Kabul. The most interesting finding was a fur of a cheetah in Mazar-e-Sharif, the first record of this species after 35 years. From the surveys can be concluded that leopards still exist in the whole range of its distribution area in Afghanistan. However they don't allow any conclusion on the population size and its threat by hunting. In contrast to the leopard there exists a recent estimation of the population size of the snow leopard, saying that there are still 100 to 200 snow leopards living in Afghanistan. On the basis of these figures as well as the numbers of furs traded annually a Population and Habitat Viability Analysis was conducted. The result of this analysis is alarming. It has to be assumed that the snow leopard will be extinct in Afghanistan within the next ten years. To improve the protection of spotted cats in Afghanistan it needs both, a better implementation of the existing legislation as well as an awareness campaign among potential clients, i. e. foreigners living in Afghanistan. The second part of this thesis deals with the question of subspecies of leopards in Afghanistan. Out of the 27 subspecies described four are believed to exist in Afghanistan. However, according to a molecularbiological revision of the species there occurs only one subspecies in Afghanistan, Panthera pardus saxicolor. To clarify the subspecies question various measures of furs had been taken in the bazars. The results revealed that the leopards in Afghanistan are the biggest of its species. However a further differentiation according to the area of origin within the country was not possible. Also the traditional differentiation on the basis of colours and patterns on the furs was not possible. In contrast to the molecularbiological investigations published not only samples of zoo animals were available in this study but also samples from the wild. The own results confim that almost all leopards from Afghanistan and Iran belong to one and the same subspecies, P. p. saxicolor. Only in the most eastern part of Afghanistan, the Indian leopard, Panthera pardus fusca, can be found. The International Studbook for the Persian Leopard was analysed. The whole population derives from a few founder animals, which were imported in the midth fifties from Iran and in the late sixties from Afghanistan. To avoid inbreeding later on the Iranian and the Afghan lines were mixed. A female imported in 1968 from Kabul to Cologne is represented in each of the more than 100 today living animals.Mixing the two lines subsequently is justified by the genetic results of this study. Recently acquired animals from the Caucasus, however, should be tested genetically before integrating them into the zoo population.
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	Publisher	University of Köln	Place of Publication	Germany	Editor
	Language	German	Summary Language		Original Title
	Series Editor		Series Title		Abbreviated Series Title
	Series Volume		Series Issue		Edition
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	Area		Expedition		Conference
	Notes				Approved	no
	Call Number	SLN @ rana @			Serial	1123
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