Abstract: Book Title: Rangeland Stewardship in Central Asia: Balancing Improved Livelihoods, Biodiversity Conservation and Land Protection, 2012. Edited by Victor Squires. Published Springer Science+Business Media. 458 p. 91 illus., 61 in color.
Addressing human–wildlife conflict is an important requisite to managing
rangelands for livestock and wildlife. Despite high altitudes, aridity, and relatively
low primary productivity, the rangelands of Central Asia support a rich and diverse
biodiversity—including the endangered snow leopard that many herders perceive
as a predator to be eliminated. Conserving this and other wildlife species requires
carefully crafted interventions aimed at curbing depredation losses and/or reducing
competition for forage, along with offering locally sustainable, environmentally
friendly income-generating activities for supplementing pastoral household livelihoods.
This is best achieved through a combination of incentives designed to foster
sound rangeland and wildlife stewardship, along penalties or disincentives targeting
herders who violate mutually agreed rules and regulations (including grazing norms
and wildlife disturbance or poaching).
When working toward the harmonious coexistence of people and wildlife,
conservationists and rangeland practitioners need to seek the cooperation and
build goodwill among herders and other stakeholders, including local government
and private industry (especially the livestock production, mining, and tourism
sectors).

Abstract: The endangered snow leopard (Panthera uncia) is widely but sparsely distributed throughout the mountainous regions of central Asia. Detailed information on the status and abundance of the snow leopard is limited because of the logistical challenges faced when working in the rugged terrain it occupies, along with its secretive nature. Camera-trapping and noninvasive genetic techniques have been used successfully to survey this felid. We compared noninvasive genetic and camera-trapping snow leopard surveys in the Gobi Desert of Mongolia. We collected 180 putative snow leopard scats from 3 sites during an 8-day period along 37.74 km of transects. We then conducted a 65-day photographic survey at 1 of these sites, approximately 2 months after scat collection. In the site where both techniques were used noninvasive genetics detected 5 individuals in only 2 days of fieldwork compared to 7 individuals observed in the 65-day camera-trapping session. Estimates of population size from noninvasive genetics ranged between 16 and 19 snow leopards in the 314.3-km2 area surveyed, yielding densities of 4.9–5.9 individuals/100 km2. In comparison, the population estimate from the 65-day photographic survey was 4 individuals (adults only) within the 264-km2 area, for a density estimate of 1.5 snow leopards/100 km2. Higher density estimates from the noninvasive genetic survey were due partly to an inability to determine age and exclude subadults, reduced spatial distribution of sampling points as a consequence of collecting scats along linear transects, and deposition of scats by multiple snow leopards on common sites. Resulting differences could inflate abundance estimated from noninvasive genetic surveys and prevent direct comparison of densities derived from the 2 approaches unless appropriate adjustments are made to the study design.

Abstract: Molecular markers that can effectively identify noninvasively collected samples and provide genetic
information are critical for understanding the distribution, status, and ecology of snow leopards (Panthera uncia). However, the low DNA quantity and quality in many
noninvasive samples such as scats makes PCR amplification and genotyping challenging. We therefore designed primers for 9 microsatellites loci previously isolated in the
domestic cat (Felis catus) specifically for snow leopard studies using noninvasive samples. The loci showed moderate levels of variation in two Mongolian snow leopard
populations. Combined with seven other loci that we previously described, they have sufficient variation (He = 0.504, An = 3.6) for individual identification and
population structure analysis. We designed a species species specific PCR assay using cytochrome b for identification of unknown snow leopard samples. These molecular markers
facilitate in depth studies to assess distribution, abundance, population structure, and landscape connectivity of this endangered species.
endangered species

Abstract: Snow leopards (Panthera uncia) are an enigmatic, high-altitude species whose challenging habitat, low population densities
and patchy distribution have presented challenges for scientists studying its biology, population structure, and genetics.
Molecular scatology brings a new hope for conservation efforts by providing valuable insights about snow leopards, including
their distribution, population densities, connectivity, habitat use, and population structure for assigning conservation units.
However, traditional amplification of microsatellites from non-invasive sources of DNA are accompanied by significant
genotyping errors due to low DNA yield and poor quality. These errors can lead to incorrect inferences in the number of
individuals and estimates of genetic diversity. Next generation technologies have revolutionized the depth of information
we can get from a species' genome. Here we used double digest restriction-site associated DNA sequencing (ddRAD-seq),
a well-established technique for studying non-model organisms, to develop a reference sequence library for snow leopards
using blood samples from five Mongolian individuals. Our final data set reveals 4504 loci with a median size range of 221 bp.
We identified 697 SNPs and low nucleotide diversity (0.00032) within these loci. However, the probability that two random
individuals will share identical genotypes is about 10-168. We developed probes for DNA capture using this sequence library
which can now be used for genotyping individuals from scat samples. Genetic data from ddRAD-seq will be invaluable for
conducting population and landscape scale studies that can inform snow leopard conservation strategies.

Abstract: Territory size is often larger for males than for females in species without biparental care. For large solitary carnivores, this is explained by males encompassing a set of female territories to monopolize their reproduction during mating (area maximization). However, males are expected to behave more like females outside of breeding, with their area utilization being dependent on the range required to secure food resources (area minimization). To examine how male and female solitary carnivores adjust their spatial organization during the year as key resources (mates and prey) change, we radio‐collared 17 pumas (Puma concolor; nine males and eight females) and 14 snow leopards (Panthera uncia; seven males and seven females) and estimated home range size and overlap on two temporal scales (annual vs. monthly). Contrary to expectation, we found no evidence that males monopolized females (the mean territory overlap between females and the focal male during the mating season was 0.28 and 0.64 in pumas and snow leopards, respectively). Although male�male overlap of annual home ranges was comparatively high (snow leopards [0.21] vs. pumas [0.11]), monthly home range overlaps were small (snow leopards [0.02] vs. pumas [0.08]) suggesting strong territoriality. In pumas, both males and females reduced their monthly home ranges in winter, and at the same time, prey distribution was clumped and mating activity increased. In snow leopards, females showed little variation in seasonal home range size, following the seasonal stability in their primary prey. However, male snow leopards reduced their monthly home range utilization in the mating season. In line with other studies, our results suggest that female seasonal home range variation is largely explained by changes in food resource distribution. However, contrary to expectations, male territories did not generally encompass those of females, and males reduced their home ranges during mating. Our results show that male and female territorial boundaries tend to intersect in these species, and hint at the operation of female choice and male mate guarding within these mating systems.